A polyketide biosynthetic gene cluster from Streptomyces antibioticus includes a LysR-type transcriptional regulator

Microbiology

Volumn 147, Issue 11, 2001, Pages 3083-3092

  Colombo, V ^a   Fernandez de Heredia M ^a   Malpartida, F ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

actII ORF4; Actinorhodin; Antibiotic; Heterologous complementation; Regulation

Indexed keywords

ACTINORHODINE; BACTERIAL DNA; DNA FRAGMENT; LYSINE; OLEANDOMYCIN; POLYKETIDE; POLYKETIDE SYNTHASE;

ANIMAL CELL; ARTICLE; ASSAY; CONTROLLED STUDY; DNA BINDING; DNA SEQUENCE; DRUG MANUFACTURE; DRUG SYNTHESIS; ENZYME ISOLATION; GENE CLUSTER; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE ISOLATION; GENE MUTATION; GENE STRUCTURE; GENETIC ANALYSIS; GENETIC CODE; GENETIC TRANSCRIPTION; HOST; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; STRAIN DIFFERENCE; STREPTOMYCES ANTIBIOTICUS; STREPTOMYCES COELICOLOR; TRANSCRIPTION REGULATION;

ACTINOBACTERIA (CLASS); ANIMALIA; BACTERIA (MICROORGANISMS); FUNGI; MYXOGASTRIA; STREPTOMYCES ANTIBIOTICUS; STREPTOMYCES COELICOLOR; UNCULTURED ACTINOMYCETE;

EID: 0035159899     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-147-11-3083     Document Type: Article

References (54)

1. Characterization of the pathway-specific positive transcriptional regulator for actinorhodin biosynthesis in Streptomyces A3(2) as a DNA-binding protein
2. Biosynthesis of anthraquinones by interspecific cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorhodin gene function
3. The regulation of antibiotic production in Streptomyces coelicolor A3(2)
4. Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI gene provides key information about the enzymology of polyketide antibiotic biosynthesis
5. A chain initiation factor common to both modular and aromatic polyketide synthases
6. Cloning and disruption of a fragment of Streptomyces halstedii DNA involved in the biosynthesis of a spore pigment
7. Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces myces spp
8. Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2)
9. The expression of Streptomyces and Escherichia coli drug-resistance determinants cloned into the Streptomyces phage φ C31
10. Spore colour in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics
11. Transcriptional analysis of the Streptomyces glaucescens tetracenomycin-C biosynthesis gene cluster
12. A comprehensive set of sequence analysis programs for the VAX
13. Nodulation of legumes by Rhizobium; the recognized roots?
14. Cloning of a Streptomyces gene for an O-methyltransferase involved in antibiotic biosynthesis
15. The act cluster contains regulatory and antibiotic export genes, direct targets for transcriptional control by the bldA transfer RNA gene of Streptomyces coelicolor
16. Nucleotide sequence and deduced function of a set of co-transcribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin
17. Lambda replacement vectors carrying polylinker sequences
18. Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor
19. Phosphorylation of the AfsR product, a global regulatory protein for secondary-metabolite formation in Streptomyces coelicolor A3(2)
20. Genetic contribution to understanding polyketide synthase
21. The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tü22: Sequence analysis and expression in a heterologous host
22. A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp
23. Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2)
24. Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumour mithramycin
25. Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumour polyketide mithramycin
26. The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster
27. The Streptomyces plasmid SCP2*: Its functional analysis and development into useful cloning vectors
28. Engineered biosynthesis of novel polyketides: Influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase
29. Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host
30. Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes
31. A relA/spoT homologous gene from Streptomyces coelicolor A3(2) controls antibiotic biosynthetic genes
32. An additional regulatory gene for actinorhodin production in Streptomyces lividans involves a LysR-type transcriptional regulator
33. Sequencing end-labelled DNA with base specific chemical cleavages
34. Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumour antibiotic of Streptomyces glaucescens
35. Use of sodium trichloroacetate and mung bean nuclease to increase sensitivity and precision during transcript mapping
36. Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)
37. Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyl transferase involved in daunorubicin biosynthesis
38. Primer-directed enzymatic amplification of DNA with a thermo-stable DNA polymerase
39. DNA sequencing with chain-terminating inhibitors
40. Molecular biology of the LysR family of transcriptional regulators
41. abaB, a putative regulator for secondary metabolism in Streptomyces
42. Molecular genetic analysis reveals a putative bifunctional polyketide cyclase/dehydrase gene from Streptomyces coelicolor and Streptomyces violaceorruber and a cyclase/O-methyltransferase from Streptomyces glaucescens
43. Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tü22
44. The stringent response in Streptomyces coelicolor A3(2)
45. Multigene families for anthracycline antibiotic production in Streptomyces peucetius
46. Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius
47. Characterisation of a Streptomyces antibioticus gene encoding a Type I polyketide synthase which has an unusual coding sequence
48. Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin biosynthesis
49. A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold
50. Codon usage in the G + T rich Streptomyces genome
51. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors
52. A set of expression plasmids for the synthesis of fused and unfused polypeptides in Escherichia coli