Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin

Journal of Bacteriology

Volumn 179, Issue 10, 1997, Pages 3354-3357

  Lombó, Felipe ^a   Siems, Karsten ^b   Braña, Alfredo F ^a   Méndez, Carmen ^a   Bindseil, Kai ^b   Salas, José A ^a  

^a UNIVERSITY OF OVIEDO   (Spain)

^b Analyticon Biotechnologies AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC ANTIBIOTIC; BACTERIAL ENZYME; MITHRAMYCIN; POLYKETIDE;

ANTINEOPLASTIC ACTIVITY; ARTICLE; BACTERIUM MUTANT; BIOSYNTHESIS; DRUG SYNTHESIS; ENZYME ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSYLATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STREPTOMYCES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES ARGILLACEUS;

EID: 0030995327     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.10.3354-3357.1997     Document Type: Article

References (25)

1. Bechthold, A., J. K. Sohng, T. M. Smith, X. Chu, and H. G. Floss. 1995. Identification of Streptomyces violaceoruber Tü22 genes involved in the biosynthesis of granaticin. Mol. Gen. Genet. 248:610-620.
2. Berlin, Y. A., M. N. Kolosov, I. V. Vasina, and I. V. Yartseva. 1968. The structure of chromocyclomycin. J. Chem. Soc. Chem. Commun. 1968:762-763.
3. Blanco, G., H. Fu, C. Méndez, C. Khosla, and J. A. Salas. 1996. Deciphering the biosynthetic origin of the aglycone of the aureolic acid group of antitumor agents. Chem. Biol. 3:193-196.
4. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
5. Distler, J., A. Ebert, K. Mansouri, K. Pissowotzki, M. Stockmann, and W. Piepersberg. 1987. Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity. Nucleic Acids Res. 15:8041-8056.
6. Gallo, M. A., J. Ward, and C. R. Hutchinson. 1996. The dnrLM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster. Microbiology 142:269-275.
7. Linton, K. J., B. W. Jarvis, and C. R. Hutchinson. 1995. Cloning of the genes encoding thymidine diphosphoglucose 4,6-dehydratase and thymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase from the erythromycin producer Saccharopolyspora erythraea. Gene 153:33-40.
8. Liu, H., and J. S. Thorson. 1994. Pathways and mechanisms in the biogenesis of novel deoxysugars by bacteria. Annu. Rev. Microbiol. 48:223-256.
9. 8a. Lombó, F. Unpublished data.
10. Lombó, F., G. Blanco, E. Fernández, C. Méndez, and J. A. Salas. 1996. Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin. Gene 172:87-91.
11. 9a. Martin, M. C. Unpublished data.
12. Merson-Davies, L. A., and E. Cundliffe. 1994. Analysis of five tylosin biosynthetic genes from the tylIBA region of the Streptomyces fradiae genome. Mol. Microbiol. 13:349-355.
13. Montanari, A., and J. P. Rosazza. 1990. Biogenesis of chromomycin A3 by Streptomyces griseus. J. Antibiot. 43:883-889.
14. Piepersberg, W. 1994. Pathway engineering in secondary metabolite-producing actinomycetes. Crit. Rev. Biotechnol. 14:251-285.
15. Pissowotzki, K., K. Mansouri, and W. Piepersberg. 1991. Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative functions of strELMB2N. Mol. Gen. Genet. 231:113-123.
16. 13a. Prado, L. Unpublished data.
17. Remers, W. A. 1979. The chemistry of antitumor antibiotics, vol. 1, p. 133-175. Wiley-Interscience, New York, N.Y.
18. 14a. Rohr, J. Personal communication.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
21. Scrutton, N. S., A. Berry, and R. N. Perham. 1990. Redesign of the coenzyme specificity of a dehydrogenase by protein engineering. Nature 343:38-43.
22. Stockmann, M., and W. Piepersberg. 1992. Gene probes for the detection of 6-deoxyhexose metabolism in secondary metabolism-producing streptomycetes. FEMS Microbiol. Lett. 90:185-190.
23. Thompson, M. W., W. R. Strohl, and H. G. Floss. 1992. Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes. J. Gen. Microbiol. 138:779-786.
24. Vara, J. A., and C. R. Hutchinson. 1988. Purification of thymidine-diphospho-D-glucose 4,6-dehydratase from an erythromycin-producing strain of Saccharopolyspora erythraea by high resolution liquid chromatography. J. Biol. Chem. 263:14992-14995.
25. Yang, K., L. Han, S. W. Ayer, and L. C. Vining. 1996. Accumulation of the angucycline antibiotic rabelomycin after disruption of an oxygenase gene in the jadomycin B biosynthetic gene cluster of Streptomyces venezuelas. Microbiology 142:123-132.