Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs

Nature Structural Biology

Volumn 8, Issue 2, 2001, Pages 166-175

  Persson, Karina ^a   Ly, Hoa D ^a   Dieckelmann, Manuela ^b   Wakarchuk, Warren W ^b   Withers, Stephen G ^a   Strynadka, Natalie C J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1,4 GALACTOSYLTRANSFERASE; CARBOHYDRATE DERIVATIVE; FLUORODEOXYGLUCOSE; GALACTOSE; GALACTOSYLTRANSFERASE; ISOENZYME; LACTOSE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GALACTOSE;

ARTICLE; BACTERIUM ADHERENCE; CARBOHYDRATE SYNTHESIS; CRYSTAL STRUCTURE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; IMMUNE RESPONSE; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; CARBOHYDRATE METABOLISM; CARBOHYDRATES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DRUG DESIGN; GALACTOSYLTRANSFERASES; GLYCOSYLTRANSFERASES; HYDROGEN BONDING; KINETICS; LACTOSE; MANGANESE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NEISSERIA MENINGITIDIS; PROTEIN STRUCTURE, SECONDARY; SEQUENCE ALIGNMENT; URIDINE DIPHOSPHATE GALACTOSE;

BACTERIA (MICROORGANISMS); NEISSERIA MENINGITIDIS;

EID: 0035151023     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/84168     Document Type: Article

References (54)

1. Epidemiology and pathogenesis of Neisseria meningitidis
2. Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis
3. Molecular mimicry of host structures by bacterial lipopolysaccharides and its contribution to disease
4. Genetic basis for biosynthesis, structure and function of meningococcal lipooligosaccharide (endotoxin)
5. A classification of nucleotide-diphospho-sugar glycosyltransferase based on amino acid sequence similarities
6. Role of paired basic residues in the expression of active recombinant galactosyltransferases from bacterial pathogen Neisseria meningitidis
7. Structure, assembly and regulation of expression of capsules in Escherichia coli
8. Selective inhibition of β-1,4- and α-1,3-galactosyltransferases: Donor sugar-nucleotide based approach
9. Catalytic mechanisms of enzymatic glycosyl transfer
10. Glycosidase mechanisms: Anatomy of a finely tuned catalyst
11. Crystal structure of the DNA modifying enzyme β-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
12. Structure of the nucleotide-diphospho-sugar transferase, SpSA from Bacillus subtilin, in native and nucleotide-complexed forms
13. Crystal structures of the bovine β4galactosyltransferse catalytic domain and its complex with uridine diphosphogalactose
14. The 1,9 Å crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis
15. Heparan/chondroitin sulfate biosynthesis: Structure and mechanism of human glucuronyltransferaseI
16. X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I; catalytic mechanism and a new protein superfamily
17. TOP: A new method for protein structure comparison and similarity searches
18. Chemo-enzymatic synthesis of fluorinated sugar nucleotide: Useful mechanistic probes for glycosyl transferases
19. Dramatic differences in the binding of UDP-galactose and UDP-glucose to UDP-galactose 4-epimerase from Escherichia coli
20. Comparison of the binding of glucose and glucose 1-phosphate derivatives to T-state glycogen phosphorylase b
21. Catalysis in the α-amylase family: X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase
22. Subsite mapping of the human pancreatic α-amylase active site through structural, kinetic, and mutagenesis techniques
23. Sequence-function relationships of prokaryotic and eukaryotic galactosyltransferases
24. Conserved domains of glycosyltransferases
25. A common motif of eukaryotic glycosyltransferases is essential for the enzymatic activity of large Clostridial cystotoxins
26. Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (Rfal)
27. Activity of the yeast MNN1 α-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases
28. Structure-function analysis of the UDP-N-acetyl-D-galactosamine: Polypeptide N-acetylgalactosaminyltransferase
29. Fringe is a glycosyltransferase that modifies Notch
30. Glycosyltransferase activity of Fringe modulates Notch-Delta interactions
31. Structural aspects of metal liganding to functional groups in proteins
32. The CCP4 suite: Programs for protein crystallography
33. Nucleophilic addition to a carbonyl group
34. Ternary complex crystal structures of glycogen phosphorylase with the transition state analog nojirimycin tetrazole and phosphate in the T and R states
35. Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: Answers to a long outstanding question
36. β-Glucosyltransferase and phophorylase reveal their common theme
37. Evolutionary link between glycogen phosphorylase and a DNA modifying enzyme
38. Mutagenesis of glycosidases
39. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin
40. A continuous spectrophotometric assay for glycosyltransferases
41. Processing of X-ray diffraction data collected in oscillation mode
42. Automated structure solution for MIR and MAD
43. DM: An automated procedure for phase improvement by density modification
44. Practical protein crystallography. A visual protein crystallographic software system for X11/XView
45. Crystallography and NMR system: A new software suit for macromolecular structure determination
46. Accurate bond and angle parameter for X-ray protein structure refinement
47. Model building and refinement practice
48. PROCHECK: A program to check the stereochemical quality of protein structures
49. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
50. Raster3D: Photorealistic molecular graphics
51. Protein folding association: Insights from the interracial and thermodynamic properties of hydrocarbons