Transposition mutagenesis in Streptomyces fradiae: Identification of a neutral site for the stable insertion of DNA by transposon exchange

Gene

Volumn 168, Issue 1, 1996, Pages 67-72

  Solenberg, Patricia J ^a   Cantwell, Cathleen A ^a   Tietz, Anthony J ^a   Mc Gilvray, Derek ^a   Queener, Stephen W ^a   Baltz, Richard H ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Conjugation; Homologous recombination; Protoplast transformation; Recombinant DNA; Tn5096; Tn5099; Tylosin

Indexed keywords

RECOMBINANT DNA; TYLOSIN;

ARTICLE; GENE INSERTION; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; NONHUMAN; PRIORITY JOURNAL; PROTOPLAST; TRANSPOSON;

EID: 0030039759     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(95)00765-2     Document Type: Article

References (23)

1. Baltz, R.H.: Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J. Gen. Microbiol. 107 (1978) 93-102.
2. Baltz, R.H.: Genetic recombination by protoplast fusion in Streptomyces. Dev. Ind. Microbiol. 21 (1980) 43-54.
3. Baltz, R.H.: Gene expression in recombinant Streptomyces. In: Smith, A. (Ed.), Gene Expression in Recombinant Microorganisms. Marcel Dekker, New York, NY, 1994, pp. 309-381.
4. Baltz, R.H., Hahn, D.R., McHenney, M.A. and Solenberg, P.J.: Transposition of Tn5096 and related transposons in Streptomyces species. Gene 115 (1992) 61-65.
5. Baltz, R.H. and Seno, E.T.: Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin. Antimicrob. Agents Chemother. 20 (1981) 214-225.
6. Baltz, R.H. and Seno, E.T.: Genetics of Streptomyces fradiae and tylosin biosynthesis. Annu. Rev. Microbiol. 42 (1988) 547-574.
7. Baltz, R.H., Seno, E.T., Stonesifer, J. and Wild, G.M.: Biosynthesis of the macrolide antibiotic tylosin: A preferred pathway from tylactone to tylosin. J. Antibiot. 36 (1983) 131-141.
8. Bierman, M., Logan, R., O'Brien, K., Seno, E.T., Rao, R.N. and Schoner, B.E.: Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116 (1992) 43-49.
9. Buluwela, L., Forster, A., Boehm, T. and Rabbitts, T.H.: A rapid procedure for colony screening using nylon filters. Nucleic Acids Res. 17 (1989) 452.
10. Cox, K.L., Fishman, S.E., Larson. J.L., Stanzak, R., Reynolds, P.A., Yeh, W.-K., Van Frank, R.M., Birmingham, V.A., Hershberger, C.L. and Seno, E.T.: The use of recombinant DNA techniques to study tylosin biosynthesis and resistance in Streptomyces fradiae. J. Nat. Products 49 (1986) 971-980.
11. Cox, K.L. and Seno, E.T.: Maintenance of cloned tylosin biosynthetic genes in Streptomyces fradiae on freely-replicating and integrative plasmid vectors. J. Cell. Biochem. Suppl. 14A (1990) 93.
12. Fishman, S.E., Cox, K., Larson, J.L., Reynolds, P.A., Seno E.T., Yeh, W.-K., Van Frank, R. and Hershberger, C.L.: Cloning genes for the biosynthesis of a macrolide antibiotic. Proc. Natl. Acad. Sci. USA 84 (1987) 8248-8252.
13. Hahn, D.R., Solenberg, P.J. and Baltz, R.H.: Tn5099, a xylE promoter probe transposon for Streptomyces spp. J. Bacteriol. 173 (1991) 5573-5577.
14. Kaster, K.R., Burgett, S. G., Rao, R.N. and Ingolia, T.D.: Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. Nucleic Acids Res. 11 (1983) 6895-6911.
15. Matsushima, P. and Baltz, R.H.: Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts. J. Bacteriol. 163 (1985) 180-185.
16. McHenney, M.A. and Baltz, R.H.: Transposition of Tn5096 from a temperature sensitive transducible plasmid in Streptomyces spp. J. Bacteriol. 173 (1991) 5578-5581.
17. Sambrook, J., Fritsch, E.F. and Maniatis, T.: Molecular Cloning. A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
18. Seno, E.T. and Baltz, R.H.: S-adenosyl-L-methionine:macrocin O-methyltransferase activities in a series of Streptomyces fradiae mutants that produce different levels of the macrolide antibiotic tylosin. Antimicrob. Agents Chemother. 21 (1982) 758-763.
19. Simon, R., Preifer, U. and Pühler, A.: A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1 (1983) 784-791.
20. Solenberg, P.J. and Baltz, R.H.: Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus. J. Bacteriol. 173 (1991) 1096-1104.
21. Solenberg, P.J. and Baltz, R.H.: Hypertransposing derivatives of the streptomycete insertion sequence IS493. Gene 147 (1994) 47-54.
22. Stonesifer, J. and Baltz, R.H. Mutagenic DNA repair in Streptomyces. Proc. Natl. Acad. Sci. USA 82 (1985) 1180-1183.
23. Yanisch-Perron, C., Vieira, J. and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 (1985) 103-199.