Complete conversion of antibiotic precursor to pristinamycin iia by overexpression of streptomyces pristinaespiralis biosynthetic genes

Nature Biotechnology

Volumn 15, Issue 4, 1997, Pages 349-353

  Sezonov, Guennadi ^a   Blanc, Véronique ^b   Bamas Jacques, Nathalie ^b   Friedmann, Annick ^a   Pernodet, Jean Luc ^a   Guérineau, Michel ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b SANOFI   (France)

Author keywords

Pristinamcin; PSSM2; Streptomyces pristinaespiralis

Indexed keywords

PRISTINAMYCIN;

ANTIGEN EXPRESSION; ARTICLE; BACTERIOSTASIS; CELL ADHESION; CHROMOSOME REPLICATION; FERMENTATION; GENE CONVERSION; GENETIC ENGINEERING; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; STEM CELL; STREPTOMYCES;

EID: 0030997180     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0497-349     Document Type: Article

References (43)

1. Preud’homme, J., Belloc, A., Charpentié, Y., and Tarridec, P. 1965. Un antibiotique formé de deux groupes de composants à synergie d’action: la pristinamycine. C. R. Acad. Sci. 260:1309–1312.
2. Preud’homme, J., Tarridec, P., and Belloc, A. 1968. Pristinamycine: isolement, caractérisation et identification des constituants. Bull. Soc. Chim. 2:585–591.
3. Vazquez, D. 1975. The streptogramin family of antibiotics. Antibiotics 3:521–534.
4. Cocito, C.G. 1979. Antibiotics of the virginiamycin family, inhibitors which contain synergistic components. Microbiol. Rev. 43:145–198.
5. Cocito, C. and Chinall, G. 1985. Molecular mechanism of action of virginiamycinlike antibiotics (synergimycins) on protein synthesis in bacterial cell-free systems. J. Antimicrob. Chemother. 16(suppl. A):35–52.
6. Aumercier, M., Bouhallab, S., Capmau, M.-L, and Le Goffic, F. 1992. RP59500: a proposed mechanism for its bactericidal activity. J. Antimicrob. Chemother. 30(suppl. A):9–14.
7. Blanc, V., Lagneaux, D., Didier, P., Gil, P., Lacroix, P., and Crouzet, J. 1995. Cloning and analysis of structural genes from Streptomyces pristinaesplralis encoding enzymes involved In the conversion of pristlnamycin IIB to pristinamycin IIA (PIIA): PIIA synthase and NADH:riboflavin 5′-phosphate oxidoreductase. J. Bacterio!. 177:5206–5214.
8. A biosynthesis. J. Bacteriol. 177:5199–5205.
9. Blanc, V., Salah-Bey, K., Folcher, M., and Thompson, C.J. 1995. Molecular characterization and transcriptional analysis of a multidrug resistance gene cloned from the pristlnamycin-producing organism, Streptomyces pristinaespiralls. Mol. Microbiol. 17:989–999.
10. Salah-Bey, K., Blanc, V., and Thompson, C.J. 1995. Stress-activated expression of a Streptomyces pristlnaespiralis multidrug resistance gene (ptr) in various Streptomyces spp. and Escherichia coli. Mol. Microbiol. 17:1001–1012.
11. Takano, E., Gramajo, H.C., Strauch, E., Andres, N., White, J., and Bibb, M.J. 1992. Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coellcolor A3(2). Mol. Microbiol. 6:2797–2804.
12. Gramajo, H.C., Takano, E., and Bibb, M.J. 1993. Stationary phase production of the antibiotic actinorhodin in Streptomyces coellcolor A3(2) is transcriptionally regulated. Mol. Microbiol. 7:837–845.
13. Hopwood, D., Malpartida, F., Kieser, H.M., Ikeda, H., Duncan, J., Fujii, I., et. al. 1985. Production of “hybrid” antibiotics by genetic engineering. Nature 314:642–644.
14. Katz, L. and Donadío, S. 1993. Polyketide synthesis: prospects for hybrid antibiotics. Annu. Rev. Microbiol. 47:875–912.
15. McDaniel, R., Khosla, E., Hopwood, D.A., and Khosla, C. 1995. Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunits. Nature 375:549–554.
16. Alvarez, M.A., Fu, H., Khosla, C, Hopwood, D.A., and Bailey, J.E. 1996. Engineered biosynthesis of novel polyketides: properties of the whiE aromatase/cyclase. Nature Biotechnology 14:335–338.
17. Thomas, D.I., Cove, J.H., Baumberg, S., Jones, CA., and Rudd, B.A.M. 1991. Plasmid effects on secondary metabolite production by a streptomycete synthesizing an anthelmintic macrolide. J. Gen. Microbiol. 137:2331–2337.
18. Pernodet, J.-L, Simonet, J.-M., and Guérineau, M. 1984. Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of Plasmid pSAM2. Mol. Gen. Genet. 198:35–41.
19. Boccard, F., Pernodet, J.-L., Friedmann, A., and Guérineau, M. 1988. Site-specific integration of plasmid pSAM2 in Streptomyces lividans and Streptomyces ambofaciens. Mol. Gen. Genet. 212:432–439.
20. Boccard, F., Smokvina, T., Pernodet, J.-L., Friedmann, A., and Guérineau, M. 1989. The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophage. EMBO J. 8:973–980.
21. Boccard, F., Smokvina, T., Pernodet, J.-L, Friedmann, A., and Guérineau, M. 1989.Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces. Plasmid 21:59–70.
22. Smokvina, T., Boccard, F., Pernodet, J.-L, Frledmann, A., and Guérineau, M. 1991. Functional analysis of the Streptomyces ambofaciens element pSAM2. Plasmid 25:40–52.
23. Hagège, J., Boccard, F., Smokvina, T., Pernodet, J.-L, Friedmann, A., and Guérineau, M. 1994. Identification of a gene encoding the replication initiator protein of the Streptomyces integrating element, pSAM2. Plasmid 31:166–183.
24. Hagège, J., Pernodet, J.-L, Friedmann, A., and Guérineau, M. 1993. Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens. Mol. Microbiol. 10:799–812.
25. Hagège, J., Pernodet, J.-L., Sezonov, G., Gerbaud, C, Friedmann, A., and Guérineau, M. 1993. Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer. J. Bacteriol. 175:5529–5538.
26. Sezonov, G, Hagège, J., Pernodet, J.-L., Friedmann, A., and Guérineau, M. 1995. Characterization of pra, a gene for replication control In pSAM2, the integrating element of Streptomyces ambofaciens. Mol. Microbiol. 17:533–544.
27. Smokvina, T., Mazodier, P., Boccard, R, Thompson, C.J., and Guérineau, M. 1990. Construction of a series of pSAM2-based integrative vectors for use in actinomycetes. Gene 94:53–59.
28. Soussy, C.J., Acar, J.F., Cluzel, R., Courvalin, P., Duval, J., Fleurette, J., et al. 1992. A collaborative study of the in-vitro sensitivity to RP 59500 of bacteria isolated in seven hospitals in France. J. Antimicrob. Chemother. 30(suppl. A):53–58.
29. Barrière, J.C., Bouanchaud, D.H., Paris, J.M., Rolin, O., Harris, N.V., and Smith, C. 1992. Antimicrobial activity against Staphylococcus aureus of semisynthetic injectable streptogramins: RP 59500 and related compounds. J. Antimicrob. Chemother. 30(suppl. A):1–8.
30. Smokvina, T. 1990. Contribution à la caractérisation de pSAM2, un élément génétique mobile de Streptomyces. Ph.D. thesis. Université de Paris-Sud, Orsay.
31. Bibb, M.J., Janssen, G.R., and Ward, J.M. 1986. Cloning and analysis of the promoter region of the erythromycin-resistance gene (ermE) of Sfrepfomyces ery-thraeus. Gene 41:E357–E368.
32. Bibb, M.J., White, J., Ward, J.M., and Janssen, G.R. 1994. The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythrasa is translated in the absence of a conventional ribosome-binding site. Mol. Microbiol. 14:533–545.
33. Mazodier, P., Thompson, C, and Boccard, F. 1990. The chromosomal integration site of the Streptomyces element pSAM2 overlaps a putative tRNA gene conserved among actinomycetes. Mol. Gen. Genet. 222:431–434.
34. Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Bruton, C.J., Kieser, H.M., et. al. 1985. Genetic manipulation of Streptomyces. A laboratory manual. The John Innes Foundation, Norwich, England.
35. Valline, W.J.S. and Baumberg, S. 1985. Cloning of a DNA fragment from Strepfomyces griseus which directs streptomycin phosphotransferase activity. J. Gen. Microbiol. 131:1657–1669.
36. Cox, K.L. and Seno, E.T. 1990. Maintenance of cloned biosynthetic genes in Strepfomyces fradtae on freely-replicating and integrative plasmid vectors. Abstracts of the 14th UCLA Symposia, inj. Cell. Biochem. (suppl. 14A): Abstract CC016.
37. Sezonov, G., Hagège, J., Pernodet, J.-L., and Guérineau, M. 1995. Biology of a Streptomyces integrating element, pSAM2. In: Proceeding of the Ninth International Symposium on the Biology of the Actinomycetes. Biotechnologia (Russia) 7–6:80–84.
38. Kuhstoss, S., Richardson, M.A., and Rao, R.N. 1989. Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2. J. Bacteriol. 171:16–23.
39. Sambrook, J., Fritsch, E.R, and Manlatis, T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY.
40. Thompson C.J., Ward, J.M., and Hopwood, D.A. 1980. DNA cloning in Streptomyces: resistance genes from antibiotic-producing species. Nature 286:525–527.
41. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
42. Pro genes and their efficacy for pSAM2 site-specific integration in Streptomyces lividans. Appl. Environ. Microbiol. 60:4279–4283.
43. Kay, R. and McPherson, J. 1987. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15:2778.