Functional complementation between mutations at two distant positions in Escherichia coli RNA polymerase as revealed by second-site suppression

Molecular and General Genetics

Volumn 264, Issue 5, 2001, Pages 531-538

  Sujatha, S ^b   Ishihama, A ^c   Chatterji, D ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

^c NATIONAL INSTITUTE OF GENETICS   (Japan)

Author keywords

E. coli RNA polymerase; Genetic suppression; Reconstitution; Subunit contacts

Indexed keywords

ASPARTIC ACID; GLYCINE; MUTANT PROTEIN; RNA POLYMERASE;

ALLELE; AMINO ACID SUBSTITUTION; ARTICLE; BETA CHAIN; CRYSTAL STRUCTURE; ENZYME RECONSTITUTION; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE MUTATION; GENE REPRESSION; GENETIC COMPLEMENTATION; GENETIC CONSERVATION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SEQUENCE ANALYSIS; TEMPERATURE SENSITIVE MUTANT;

AMINO ACID SEQUENCE; ASPARTIC ACID; CHROMATOGRAPHY, GEL; CRYSTALLOGRAPHY, X-RAY; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GLYCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SUPPRESSION, GENETIC; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035140002     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380000353     Document Type: Article

References (45)

1. A suppressor of an RNA polymerase II mutation of Saccharomyces cerevisiae encodes a subunit common to RNA polymerases I, II, and III
2. Construction of plasmids carrying the CI gene of bacteriophage
3. Fluorescence spectroscopy analysis of active and regulatory sites of RNA polymerase
4. Identification of the target of a transcription activator protein by protein-protein photocrosslinking
5. Reconstitution of RNA polymerase
6. 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron EDTA protein footprinting
7. Plasmid transformation of Escherichia coli and other bacteria
8. Mechanisms of suppression
9. Functional specialization within the α-subunit of Escherichia coli RNA polymerase
10. Structure and function of bacterial sigma factors
11. Mapping domains involved in macromolecular interactions: A novel protein footprinting approach
12. Subunit topography of RNA polymerase from Escherichia coli. A cross-linking study with bifunctional reagents
13. Bipartite functional map of the Escherichia coli RNA polymerase α-subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP
14. Sequence analysis of two temperature-sensitive mutations in the alpha subunit gene (rpoA) of E. coli RNA polymerase
15. Subunit assembly of Escherichia coli RNA polymerase
16. Temperature sensitive mutations in the α subunit of Escherichia coli RNA polymerase
17. Structural and functional alterations of a colicin-resistant mutant of Omp F porin from Escherichia coli
18. Functional map of the alpha subunit of Escherichia coli RNA polymerase: Amino acid substitution within the amino-terminal assembly domain
19. Functional map of the alpha subunit of Escherichia coli RNA polymerase: Insertion analysis of the amino-terminal assembly domain
20. Subunit assembly in vivo of E. coli RNA polymerase: Role of amino-terminal assembly domain of alpha subunit
21. 54 and the β subunit of RNA polymerase
22. Low copy number plasmids for regulated low level expression of Escheriehia coli with blue/ white insert screening capability
23. Target of transcriptional activation function of phage lambda at cI protein
24. Mutations in rpoA affect expression of anaerobically regulated genes in Salmonella typhimurium
25. Genetic exploration of interactive domains in RNA polymerase II subunits
26. 4-fold amplification of Escherichia coli minor protein
27. 70 -α crosslink to the CTD of α subunit
28. Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: An assessment using mutations in yeast cytochrome c oxidase subunits I and II
29. Dimeric association of E. coli RNA polymerase alpha subunits, studied by cleavage of singlecysteine alpha subunits conjugated to iron-(S)-1-[(bromoacetamido)benzyl]ethyene diaminetetraacetate
30. Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery
31. Most conserved regions of the E. coli primary sigma factor are involved in interaction with RNA polymerase core enzyme
32. Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature
33. Mapping of subunitsubunit contact surfaces on the β subunit of Escherichia coli RNA polymerase
34. Mapping of subunit-subunit contact surfaces on the β subunit of Escherichia coli RNA polymerase
35. Specific fluorescent labeling of two functional domains in RNA polymerase α subunit
36. Suppression mechanisms: Themes from variations
37. Genetic evidence for interaction between cluster I and Cluster III rifampicin resistant mutations
38. Identifying interacting regions of the β subunit of Escherichia coli RNA polymerase
39. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalities and weight matrix choice
40. Termination-altering amino acid substitutions in the β′ subunit of Escherichia coli RNA polymerase identify regions in RNA chain elongation
41. Suppressor analysis of temperature sensitive mutation of the largest subunit of RNA polymerase in Saccharomyces cerevisiae: A suppressor gene encodes the secondlargest subunit of RNA polymerase I
42. Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain
43. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3Å resolution