Identifying interacting regions in the β subunit of Escherichia coli RNA polymerase

Journal of Molecular Biology

Volumn 258, Issue 2, 1996, Pages 213-223

  Tavormina, Patricia L ^a,b   Reznikoff, William S ^a   Gross, Carol A ^a,c  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

E. coli RNA polymerase; Intragenic suppression; rpoB; Transcription elongation; Transcription termination

Indexed keywords

RNA POLYMERASE;

ALLELE; ARTICLE; BETA CHAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE MAPPING; GENE MUTATION; GENE REPRESSION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION TERMINATION;

EID: 0029990018     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0244     Document Type: Article

References (31)

1. Adams, A. E. M., Botstein, D. & Drubin, D. G. (1989). A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation. Science, 243, 231-233.
2. Borukhov, S., Lee, J. & Goldfarb, A. (1991). Mapping of a contact for the RNA 3′ terminus in the largest subunit of RNA polymerase. J. Biol. Chem. 266(35), 23932-23935.
3. Burgess, R., Erickson, B., Gentry, D., Gribskov, M., Hager, D., Lesley, S., Strickland, M. & Thompson, N. (1987). Bacterial RNA Polymerase Subunits and Genes (Reznikoff, W. S., Burgess, R. R., Dahlberg, J. E., Gross, C. A., Record, M. T. & Wickens, M. P., eds), Elsevier, New York, USA.
4. Casadaban, M. J. & Cohen, S. N. (1980). Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138, 179-207.
5. Chamberlin, M. J., Nierman, W. E., Wiggs, J. & Neff, N. (1979). A quantitative assay for bacterial RNA polymerases. J. Biol. Chem. 254, 10061-10069.
6. Fisher, R. F. & Yanofsky, C. (1983). Mutations of the β subunit of RNA polymerase alter both transcription pausing and transcription termination in the trp operon leader region. J. Biol. Chem. 258, 8146-8150.
7. Hartman, P. E. & Roth, J. R. (1973). Mechanisms of suppression. Adv. Genet. 17, 1-105.
8. Hollingsworth, N. & Johnson, A. (1993). A conditional allele of the Saccharomyces cerevisae HOP1 gene is suppressed by overexpression of two other meiosis specific genes: RED1 and REC104. Genetics, 133(4), 785-797.
9. Jin, D. J. & Gross, C. A. (1988). Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202, 45-58.
10. Jin, D. J., Cashel, M., Friedman, D., Nakamura, Y., Walter, W. & Gross, C. A. (1988). The effects of rifampicin resistant rpoB mutations on antitermination and interaction with NusA in Escherichia coli. J. Mol. Biol. 204, 247-261.
11. Landick, R., Colwell, A. & Stewart, J. (1990a). Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit β-subunit assembly into RNA polymerase. J. Bacteriol. 172, 2844-2854.
12. Landick, R., Stewart, J. & Lee, D. N. (1990b). Amino acid changes in conserved regions of the β subunit of Escherichia coli. Genes Dev. 4, 1623-1636.
13. Lissemore, J. L., Currie, P. D., Turk, C. M. & Maine, E. M. (1993). Intragenic dominant suppressors of glp-1, a gene essential for cell-signaling in Caenorhabditis elegans, support a role for cdc10/sw16/ankyrin motifs in Glp 1 function. Genetics, 35(4), 1023-1034.
14. Luck, D. J. L. (1984). Genetic and biochemical dissection of the eukaryotic flagellum. J. Cell. Biol. 98, 789-794.
15. Mandel, M. & Higa, A. (1970). Calcium-dependent bacteriophage DNA infection. J. Mol. Biol. 53, 159-162.
16. Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Martin, C., Okamura, S. & Young, R. (1990). Genetic exploration of interactive domains in RNA polymerase II subunits. Mol. Cell. Biol. 10(5), 1908-1914.
18. McClure, R. M. & Cech, C. L. (1978). On the mechanism of rifampicin inhibition of RNA polymerase. J. Biol. Chem. 253, 8949-8956.
19. McKenney, K., Shimatake, H., Court, D., Schmeissner, U., Brady, C. & Rosenberg, M. (1981). A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. In Gene Amplification and Analysis II: Analysis of Nucleic Acids by Enzymatic Methods (Chirikjian, J. G. & Papas, T. S., eds), pp. 383-415, Elsevier-North, Holland.
20. Miller, J. H. (1972). Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Morrison, D. A. (1979). Transformation and preservation of competent bacterial cells by freezing. In Methods in Enzymology (Wu, R., ed.), vol. 68, pp. 326-331, Academic Press, New York, USA.
22. Mustaev, A., Kashlev, M., Lee, J. Y., Polyakov, A., Lebedev, A., Zalenskaya, K., Grachev, M., Goldfarb, A. & Nikiforov, V. (1991). Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase. J. Biol. Chem. 266(35), 23927-23931.
23. Nomura, M., Morgan, E. A. & Jaskunas, S. R. (1977). Genetics of bacterial ribosomes. Annu. Rev. Genet. 11, 297-347.
24. Oosawa, K., Hess, J. F. & Simon, M. I. (1988). Mutants defective in bacterial chemotaxis show modified protein phosphorylation. Cell, 53, 89-96.
25. Sagitov, V., Nikiforov, V. & Goldfarb, A. (1993). Dominant lethal mutations near the 5′ substrate binding site affect RNA polymerase propagation. J. Biol. Chem. 268(3), 2195-2202.
26. Severinov, K., Suoshko, M., Goldfarb, A. & Nikiforov, V. (1993). Rifampicin region revisited. New rifampicin-resistant and streptolydigin-resistant mutants in the β subunit of Escherichia coli RNA polymerase. J. Biol. Chem. 268, 14820-14825.
27. Severinov, K., Soushko, M., Goldfarb, A. & Nikiforov, V. (1994). RifR mutations in the beginning of the Escherichia coli rpoB gene. Mol. Gen. Genet. 244, 120-126.
28. Singer, M. H., Jin, D. J., Walter, W. A. & Gross, C. A. (1993). Genetic evidence for the interaction between cluster I and cluster III rifampicin resistant mutations. J. Mol. Biol. 231, 1-5.
29. Tavormina, P., Landick, R. & Gross, C. A. (1996). Isolation and characterization of defective Escherichia coli RNA rpoB mutations. J. Bacteriol. In the press.
30. Weilbaecher, R., Hebron, C., Feng, G. & Landick, R. (1994). Termination-altering amino acid substitutions in the β′ subunit of Escherichia coli RNA polymerase identify regions involved in RNA chain elongation. Genes Dev. 8, 2913-2927.
31. Zabarovsky, E. R. & Winberg, G. (1990). Highly efficient electroporation of ligated DNA into bacteria. Nucl. Acids Res. 18(19), 5912.