Somatic hypermutation of immunoglobulin κ transgenes: Association of mutability with demethylation

Immunology and Cell Biology

Volumn 79, Issue 1, 2001, Pages 18-22

  Jolly, Christopher J ^a,b   Neuberger, Michael S ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b CENTENARY INSTITUTE   (Australia)

Author keywords

DNA methylation; Germinal centre; Immunoglobulin mutation; Mouse; Mus musculus; Transgene

Indexed keywords

IMMUNOGLOBULIN KAPPA CHAIN;

ARTICLE; DEMETHYLATION; GENE AMPLIFICATION; GENE MUTATION; GENE TARGETING; GENETIC ASSOCIATION; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; TRANSGENE;

ANIMALS; B-LYMPHOCYTES; CPG ISLANDS; DNA; DNA METHYLATION; GENE REARRANGEMENT; GENES, IMMUNOGLOBULIN; HUMANS; IMMUNOGLOBULINS; MICE; MICE, TRANSGENIC; MUTATION; PEYER'S PATCHES; SULFITES; TRANSGENES;

MUS MUSCULUS; MUSCULUS;

EID: 0035131689     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1711.2001.00968.x     Document Type: Article

References (28)

1. Elements regulating somatic hypermutation of an immunoglobulin κ gene: Critical role for the intron enhancer/matrix attachment region
2. Somatic hypermutation of immunoglobulin genes is linked to transcription initiation
3. The 5′ hypermutation boundary of κ chains is independent of local and neighbouring sequences and related to the distance from the initiation of transcription
4. The transcriptional promoter regulates hypermutation of the antibody heavy chain locus
5. Multiple sequences from downstream of the Jκ cluster can combine to recruit somatic hypermutation to a heterologous, upstream mutation domain
6. Cells strongly expressing Igκ transgenes show clonal recruitment of hypermutation: A role for both MAR and the enhancers
7. Somatic hypermutation in the heavy chain locus correlates with transcription
8. Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process
9. Evaluation of the role of the 3′α heavy chain enhancer [3′α E(hs1,2)] in Vh gene somatic hypermutation
10. Somatic hypermutation of immunoglobulin κ may depend on sequences 3′ of Cκ and occurs on passenger transgenes
11. Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin light-chain transgenes
12. Methylation analysis using bisulfite genomic sequencing: Application to small numbers of intact cells
13. A new DNA sequence assembly program
14. Immunoglobulin gene expression and DNA methylation in murine pre-B cell lines
15. Specific 5′ and 3′ regions of the μ-chain gene are undermethylated at distinct stages of B-cell differentiation
16. Demethylation of the constant region genes of immunoglobulins reflects the differentiation state of the B cell
17. Developmental pattern of gene-specific DNA methylation in the mouse embryo and germ line
18. The coupling between enhancer activity and hypomethylation of κ immunoglobulin genes is developmentally regulated
19. Methylation status and DNase I sensitivity of immunoglobulin genes: Changes associated with rearrangement
20. κ chain monoallelic demethylation and the establishment of allelic exclusion
21. Immunoglobulin heavy chain genes: Demethylation accompanies class switching
22. Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching
23. Methylation patterns of immunoglobulin genes in lymphoid cells: Correlation of expression and differentiation with undermethylation
24. CpNpG methylation in mammalian cells
25. Both DNA strands of antibody genes are hypermutation targets
26. Monitoring and interpreting the intrinsic features of somatic hypermutation
27. B cell-specific demethylation: A novel role for the intronic κ chain enhancer sequence
28. A role for nuclear NF-κB in B-cell-specific demethylation of the Igκ locus