A bicistronic retroviral vector to introduce drug resistance genes into human umbilical cord blood CD34+ cells to improve combination chemotherapy tolerance

Chinese Medical Journal

Volumn 114, Issue 1, 2001, Pages 25-29

  Wang, Jishi ^a   Chen, Zixing ^a   Xia, Xueming ^a   Lu, Daru ^b   Xue, Jinglun ^b   Ruan, Changgeng ^a  

^a THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Aldehyde dehydrogenase class1gene (ALDH 1); Cord blood; Gene co expression; Gene therapy; Hematopoietic stem cells; Multidrug resistance gene (MDR1); Retrovirus vector

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; 4 HYDROPEROXYCYCLOPHOSPHAMIDE; ALDEHYDE DEHYDROGENASE; CD34 ANTIGEN; COMPLEMENTARY DNA; DAUNORUBICIN; ETOPOSIDE; GLYCOPROTEIN P; PACLITAXEL; VINCRISTINE; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BONE MARROW SUPPRESSION; CANCER COMBINATION CHEMOTHERAPY; CELL PROTECTION; CELL STRAIN 3T3; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG TOLERANCE; GENE TRANSFER; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; RETROVIRUS; UMBILICAL CORD BLOOD;

ALDEHYDE DEHYDROGENASE; ANIMALS; ANTIGENS, CD34; ANTINEOPLASTIC COMBINED CHEMOTHERAPY PROTOCOLS; CELL LINE; DRUG RESISTANCE, NEOPLASM; FETAL BLOOD; GENETIC VECTORS; HEMATOPOIETIC STEM CELLS; HUMANS; ISOENZYMES; MICE; P-GLYCOPROTEIN; RETROVIRIDAE;

EID: 0035095233     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Hesdorffer C, Ayello J, Ward M, et al. Phase I trial of retroviral-mediated transfer of the human MDR1 gene as marrow chemoprotection in patients undergoing high-dose chemotherapy and autologous stem-cell transplantation. J Clin Oncol 1998;16:165-172.
2. Moscow JA, Huang H, Carter C, et al. Engraftment of MDR1 and NeoR gene-transdced hematopoietic cells after breast cancer chemotherapy. Blood 1999;94:52-61.
3. Hsu LC, Chang WC, Yoshida A, et al. Genomic structure of the human cytosolic aldehyde-dehydrogenase gene. Gemomics 1989;5: 857-863.
4. Michele M, Suzy S, Raffaella S, et al. Induction of cyclophosphamide- resistance by aldehydedehydrogenase gene transfer. Blood 1996;87:1097-1103.
5. Sreerama L, Sladek NE. Cellular levels of class 1 and class 3 aldehyde dehydrogenases and certain other drug-metabolizing enzymes in human breast malignancies. Clin Cancer Res 1997;3: 1901-1914.
6. Jans M, Mark S, Brian G, et al. In vitro selection for K562 cells with higher retrovirally mediated copy number of aldehyde dehydrogenase class-1 and higher resistance to 4-hydreoperoxycyclophosphamide. Hum Gene Ther 1998;9:611-619.
7. Suzuki M, Sugimoto Y, Tsukahara S, et al. Retroviral coexpression of two different types of drug resistance genes to protect normal cells from combination chemotherapy. Clin Cancer Res 1997;3:947-954.
8. Lynch CM, Miller AD. Production of high-titer helper virus-free retroviral vectors by cocultication of packaging cells with different host rangs. J Virol 1991;65:3887-3890.
9. Moreb J, Schweder M, Suresh A, et al. Overexpression of the human aldehyde dehydrogenase class-1 results in increased resistance to 4- hydroperpxycycy- clophosphamide. Cancer Gene Ther 1996;3:24-30.
10. Kleiman SE, Pastan I, Puck JM, et al. Characterization of an MDR1 retroviralbicistronic vector for correction of X-linked severe combined immunodeficiency. Gene Ther 1998;5:671-676.
11. Suzuki M, Sugimoto Y, Tsukahara S, et al. Retroviral coexpression of two different types of drug resistance genes to protect normal cells from combination chemotherapy. Clin Cancer Res 1997;3:947-954.
12. Hildinger G, Fehse B, Hgewisch-Becker S, et al. Dominant selection of hematopoieticprogenitor cells with retroviral MDR1 co-expression vectors. Hum Gene Ther 1998;9:33-42.