An immunodominant neutralization epitope on the 'thumb' subdomain of human immunodeficiency virus type 1 reverse transcriptase revealed by phage display antibodies

Journal of General Virology

Volumn 82, Issue 4, 2001, Pages 813-820

  Ohba, H ^a   Soga, T ^a   Tomozawa, T ^a   Nishikawa, Y ^a   Yasuda, A ^a   Kojima, A ^a   Kurata, T ^a   Chiba, J ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY; RNA DIRECTED DNA POLYMERASE; RNA DIRECTED DNA POLYMERASE INHIBITOR; VIRUS DNA; VIRUS ENZYME;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL LINE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME BINDING; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SPECIFICITY; EPITOPE MAPPING; FEMALE; GENE LIBRARY; GENE TARGETING; GENE THERAPY; HUMAN IMMUNODEFICIENCY VIRUS 1; HYBRIDOMA; IMMUNIZATION; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; SPLEEN CELL; VACCINIA VIRUS; VIRUS RECOMBINANT;

ANIMALIA; DNA VIRUSES; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES; VACCINIA; VACCINIA VIRUS;

EID: 0035061824     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-82-4-813     Document Type: Article

References (21)

1. Combinatorial immunoglobulin libraries on surface phage (phabs): Rapid selection of antigen-specific Fabs
2. Structure/function studies of human immunodeficiency virus type 1 reverse transcriptase. Alanine scanning mutagenesis of an alpha helix in the thumb subdomain
3. Reduced frameshift fidelity and processivity of HIV-1 reverse transcriptase mutants containing alanine substitutions in helix H of the thumb subdomain
4. A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals
5. The selection of intracellular antibodies
6. A novel neutralization epitope on the 'thumb' subdomain of human immunodeficiency virus type 1 reverse transcriptase revealed by a monoclonal antibody
7. Generation of neutralizing antibody to the reverse transcriptase of human immunodeficiency virus type 1 by immunizing of mice with an infectious vaccinia virus recombinant
8. Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer
9. Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family
10. The 'helix clump' in HIV-1 reverse transcriptase: A new nucleic acid-binding motif common in nucleic acid polymerases
11. Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions
12. Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: An ultrastructural study
13. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces
14. Crystal structure at 3·5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor
15. Nonviral gene therapy: The promise of genes as pharmaceutical products
16. Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety
17. Residues in the alphaH and alphaI helices of the HIV-1 reverse transcriptase thumb subdomain required for the specificity of RNase H-catalyzed removal of the polypurine tract primer
18. Phage display of combinatorial antibody libraries
19. Intracellular antibodies (intrabodies) for gene therapy of infectious diseases
20. Rapid detection of antigen binding by antibody fragments expressed in the periplasm of Escherichia coli
21. Characteristics of an attenuated vaccinia virus strain, LC16m0, and its recombinant virus vaccines