Inhibitory sequences in the N-terminus of the double-stranded-RNA-dependent protein kinase, PKR, are important for regulating phosphorylation of eukaryotic initiation factor 2α (eIF2α)

European Journal of Biochemistry

Volumn 268, Issue 4, 2001, Pages 1143-1153

  Vattem, K M ^a   Staschke, K A ^a   Zhu, S ^a   Wek, R C ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

eIF2; Inhibitory sequences; PKR; Translation

Indexed keywords

DOUBLE STRANDED RNA; HEPARIN; INITIATION FACTOR 2; INTERFERON; PROTEIN KINASE; SINGLE STRANDED DNA;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME REGULATION; GENE ACTIVATION; GENE DELETION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; TRANSLATION INITIATION;

EIF-2 KINASE; ENZYME ACTIVATION; ESCHERICHIA COLI; EUKARYOTIC INITIATION FACTOR-2; HEPARIN; HUMANS; KINETICS; PHOSPHORYLATION; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, TERTIARY; RNA, DOUBLE-STRANDED; SEQUENCE DELETION; TRANSFECTION;

EUKARYOTA; MAMMALIA;

EID: 0035058685     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.01979.x     Document Type: Article

References (64)

1. Protein Kinases that phosphorylate eIF2 and eIF2B, and their role in eukaryotic cell translational control
2. PKR: A new name and new roles
3. eIF-2 kinases: Regulators of general and gene-specific translation initiation
4. Molecular mechanisms of interferon resistance mediated by viral-inhibition of PKR, the interferon-induced protein kinase
5. PKR: A sentinel kinase for cellular stress
6. Double stranded RNA-activated protein kinase mediates virus induced apoptosis: A new role for an old actor
7. The pathway and mechanism of eukaryotic protein synthesis
8. Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase
9. Tumor suppressor function of the interferon-induced double-stranded RNA-activated protein kinase
10. Molecular mechanisms responsible for malignant transformation by regulatory and catalytic domain variants of the interferon-induced enzyme RNA-dependent protein kinase
11. The interferon-induced double-stranded RNA-activated protein kinase induces apoptosis
12. The apoptosis pathway triggered by the interferon-induced protein kinase PKR requires the third basic domain, initiates upstream of Bcl-2 and involves ICE-like proteases
13. Phosphorylation of eukaryotic initiation factor 2 mediates apoptosis in response to activation of the double-stranded RNA-dependent protein kinase
14. Activation of the dsRNA-dependent protein kinase, PKR, induces apoptosis through FADD-mediated death signaling
15. Expression of a PKR dominant-negative mutant in myogenic cells interferes with the myogenic process
16. Regulatable expression of the interferon-induced double-stranded RNA dependent protein kinase PKR induces apoptosis and fas receptor expression
17. Mechanism of interferon action: Identification of a RNA binding domain within the N-terminal region of the human RNA-dependent P1/eIF-2 α protein kinase
18. Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase
19. Two RNA-binding motifs in the double-stranded RNA-activated protein kinase, DAI
20. Functional expression and RNA binding analysis of the interferon-induced, double-stranded RNA-activated, 68,000-Mr protein kinase in a cell-free system
21. Mechanism of interferon action motif I of the interferon-induced, RNA-dependent protein kinase (PKR) is sufficient to mediate RNA-binding activity
22. Mutational analysis of the double-stranded RNA (dsRNA) binding domain of the dsRNA-activated protein kinase, PKR
23. Construction and expression of an enzymatically active human-mouse chimeric double-stranded RNA-dependent protein kinase
24. Requirement of PKR dimerization mediated by specific hydrophobic residues for its activation by double-stranded RNA and its antigrowth effects in yeast
25. A conserved double-stranded RNA-binding domain
26. NMR solution structure of a dsRNA binding domain from Drosophila Staufen protein reveals homology to the N-terminal domain of ribosomal protein S5
27. Structure of the dsRNA binding domain of E. coli RNase III
28. Structure of the double-stranded RNA-binding domain of the protein kinase PKR reveals the molecular basis of its dsRNA-mediated activation
29. Molecular basis of double-stranded RNA-protein interactions: Structure of a dsRNA-binding domain complexed with dsRNA
30. RNA recognition by a Staufen double-stranded RNA-binding domain
31. Role of the amino-terminal residues of the interferon-induced protein kinase in its activation by double-stranded RNA and heparin
32. Double-stranded-RNA-dependent protein kinase and TAR RNA-binding protein form homo- and heterodimers in vivo
33. The interferon-inducible double-stranded RNA-activated protein kinase self-associates in vitro and in vivo
34. Specific mutations near the amino terminus of double-stranded RNA-dependent protein kinase (PKR) differentially affects its double-stranded RNA binding and dimerization properties
35. A model for the double-stranded RNA (dsRNA)-dependent dimerization and activation of the dsRNA-acting protein kinase PKR
36. Double-stranded RNA-independent dimerization of interferon-induced protein kinase PKR and inhibition of dimerization by the cellular P58IPK inhibitor
37. Autophosphorylation sites participate in the activation of the double-stranded-RNA-activated protein kinase PKR
38. Autophosphorylation in the activation loop is required for full kinase activity in vivo of human and yeast eukaryotic initiation factor 2α kinases PKR and GCN2
39. Ribosome targeting of PKR is mediated by two double-stranded RNA-binding domains and facilitates in vivo phosphorylation of eukaryotic initiation factor-2
40. Identification and requirement of three ribosome binding domains in dsRNA-dependent protein kinase (PKR)
41. Inhibition of the double-stranded RNA-dependent protein kinase PKR by mammalian ribosomes
42. Translational control in cells infected with influenza virus and reovirus
43. Interactions between viruses and the cellular machinery for protein synthesis
44. Proteins that interact with PKR
45. Phosphorylation of initiation factor 2 alpha by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast
46. Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2
47. Plasmid vectors carrying the replication origin of filamentous single-stranded phages
48. Precise gene fusion by PCR
49. Purification and activation of the double-stranded RNA-dependent eIF-2 kinase DAI
50. Glucose limitation induces GCN4 translation by activation of Gcn2 protein kinase
51. Autophosphorylation of the protein kinase dependent on double-stranded RNA
52. The double-stranded RNA-dependent protein kinase is also activated by heparin
53. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon
54. Structural requirements for double-stranded RNA binding, dimerization, and activation of the human eIF-2α kinase DAI in Saccharomyces cerevisiae
55. Identification and characterization of pancreatic eukaryotic initiation factor 2 α-subunit kinase, PEK, involved in translation control
56. Mammalian eukaryotic initiation factor 2 alpha kinases functionally substitute for GCN2 protein kinase in the GCN4 translational control mechanism of yeast
57. Mechanism of interferon action: Autoregulation of RNA-dependent P1/eIF-2 α protein kinase (PKR) expression in transfected mammalian cells
58. Translational regulation by the interferon-induced double-stranded-RNA-activated 68-kDa protein kinase
59. Mutants of the RNA-dependent protein kinase (PKR) lacking double-stranded RNA binding domain I can act as transdominant inhibitors and induce malignant transformation
60. Characterization of the interactions between double-stranded RNA and the double-stranded RNA binding domain of the interferon induced protein kinase
61. A dynamically tuned double-stranded RNA binding mechanism for the activation of antiviral kinase PKR
62. Double-stranded (ds) RNA binding and not dimerization correlates with the activation of the dsRNA-dependent protein kinase (PKR)
63. Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase
64. Double-stranded RNA-activated protein kinase (PKR) is regulated by 60S ribosomal subunit protein L18