Cell type-specific differences in the coupling of recombinant mGlu1α receptors to endogenous G protein sub-populations

Neuropharmacology

Volumn 40, Issue 5, 2001, Pages 645-656

  Selkirk, Julie V ^a   Price, Gary W ^b   Nahorski, Stefan R ^a   Challiss, R A John ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

35S GTP S binding; 3H Quisqualate binding; G protein; G protein specific immunoprecipitation; Metabotropic glutamate receptor (mGlu1 ); Pertussis toxin; Phosphoinositide turnover

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); ISOPROTEIN; METABOTROPIC RECEPTOR; METABOTROPIC RECEPTOR 1ALPHA; PERTUSSIS TOXIN; PHOSPHOLIPASE C; QUISQUALIC ACID; RECOMBINANT RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING KINETICS; CELL MEMBRANE; CELL SPECIFICITY; CELL STRAIN BHK; CELL TYPE; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME REGULATION; IMMUNOPRECIPITATION; IMMUNOREACTIVITY; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING;

ANIMALS; BLOTTING, WESTERN; CELL MEMBRANE; CELLS, CULTURED; CHO CELLS; CRICETINAE; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); INOSITOL PHOSPHATES; NEURONS; PERTUSSIS TOXIN; PRECIPITIN TESTS; QUISQUALIC ACID; RECEPTORS, METABOTROPIC GLUTAMATE; VIRULENCE FACTORS, BORDETELLA;

EID: 0035051881     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(00)00208-2     Document Type: Article

References (40)

1. 2+ signal transduction
2. 35S]GTPγS binding
3. 35S]GTPγS binding and Gα-specific immunoprecipitation
4. Signal transduction and pharmacological characteristics of a metabotropic glutamate receptor, mGluR1, in transfected CHO cells
5. Specific G protein and μ-opioid receptor internalization caused by morphine, DAMGO and endomorphin I
6. Enhanced type 1α metabotropic glutamate receptor-stimulated phosphoinositide signalling after pertussis toxin treatment
7. αs proteins
8. Assessment of neuronal phosphoinositide turnover and its disruption by lithium
9. (+)-2-Methyl-4-carboxyphenylglycine (LY367385) selectively antagonises metabotropic glutamate mGluR1 receptors
10. Pharmacology and functions of metabotropic glutamate receptors
11. Cloning and expression of the human metabotropic glutamate receptor mGluR1α: Enhanced coupling on co-transfection with a glutamate transporter
12. Activation of the extracellular signal-regulated kinase 2 by metabotropic glutamate receptors
13. Glutamate mediates an inhibitory postsynaptic potential in dopamine neurons
14. Role of the second and third intracellular loops of metabotropic glutamate receptors in mediating dual signal transduction activation
15. Analysis of receptor-stimulated and basal guanine nucleotide binding to membrane G proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis
16. qα activity
17. 2+ signalling in an inducible cell expression system
18. Effects of varying the expression level of recombinant human mGlu1α receptors on the pharmacological properties of agonists and antagonists
19. Heterologous mammalian expression systems for investigating the properties of metabotropic glutamate receptors
20. Complex involvement of pertussis toxin-sensitive G proteins in the regulation of type 1α metabotropic glutamate receptor signalling in baby hamster kidney cells
21. 2+ channels
22. i proteins
23. qα pathway in C. elegans
24. 2+ channels via multiple transduction pathways in HEK 293 cells
25. Inhibition of N-linked glycosylation of the human type 1α metabotropic glutamate receptor by tunicamycin: Effects on cell-surface receptor expression and function
26. Coupling of inositol phospholipid metabolism with excitatory amino acid recognition sites in rat hippocampus
27. The activation of inositol phospholipid metabolism as a signal-transduction system for excitatory amino acids in primary cultures of cerebellar granules cells
28. Expression and purification of the extracellular ligand binding region of metabotropic glutamate receptor subtype 1
29. A comparison of two alternatively spliced forms of a metabotropic glutamate receptor coupled to phosphoinositide turnover
30. The metabotropic glutamate receptors: Structure and functions
31. Domains involved in the specificity of G protein activation in phospholipase C-coupled metabotropic glutamate receptor
32. New perspectives for the development of selective metabotropic glutamate receptor ligands
33. 3H]quisqualate binding to rat recombinant metabotropic glutamate 1a and 5a receptors and to rat brain sections
34. Switch from facilitation to inhibition of excitatory synaptic transmission by group I mGluR desensitisation
35. Pharmacological agents acting at subtypes of metabotropic glutamate receptors
36. Differential coupling to endogenous G protein subpopulations in CHO and BHK cell-lines recombinantly expressing mGlu1α receptors
37. Glutamate stimulates inositol phosphate formation in striatal neurones
38. Metabotropic glutamate receptor subtype 1a activates adenylate cyclase when expressed in baby hamster kidney cells
39. A pharmacological characterization of the mGluR1a subtype of the metabotropic glutamate receptor expressed in a cloned baby hamster kidney cell line
40. o subtype of G proteins in SH-SY5Y cells