Activation of a cryptic 5′ splice site by U1 snRNA

RNA

Volumn 7, Issue 3, 2001, Pages 342-350

  Alvarez, Consuelo J ^a,b   Wise, Jo Ann ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

S. cerevisiae; Splice site selection; U5 snRNA; U6 snRNA

Indexed keywords

SMALL NUCLEAR RNA;

ALLELE; ARTICLE; BASE PAIRING; CONTROLLED STUDY; GENE ACTIVATION; INTRON; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; RNA SPLICING; SCHIZOSACCHAROMYCES POMBE;

BASE PAIRING; CDC2 PROTEIN KINASE; INTRONS; MUTATION; RNA PRECURSORS; RNA SPLICING; RNA, FUNGAL; RNA, MESSENGER; RNA, SMALL NUCLEAR; SCHIZOSACCHAROMYCES;

SCHIZOSACCHAROMYCES POMBE;

EID: 0035032822     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201002448     Document Type: Article

References (41)

1. 5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice site region, not by the conserved 5′ GU
2. Mutational analysis of U1 function in S. pombe: Pre-mRNAs differ in the extent and nature of their requirements for this snRNA in vivo
3. Exon recognition in vertebrate splicing
4. Developmentally regulated alternative splicing of transcripts from the Drosophila homeobox gene Antennapedia can produce four different proteins
5. Finding splice sites within a wilderness of RNA
6. Splicing of precursors to messenger RNAs by the spliceosomes
7. Recognition of mutant and cryptic 5′ splice sites by the U1 small ribonucleoprotein in vitro
8. Suppression of mammalian 5′ splice site defects by U1 small nuclear RNAs from a distance
9. Mutations in the conserved loop of human U5 snRNA generate the use of novel cryptic 5′ splice sites in vivo
10. A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1 independent splicing
11. Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing
12. Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe
13. Comparative studies of Drosophila Antennapedia genes
14. U1 snRNA promotes the use of nearby 5′ splice sites by U6 snRNA in mammalian cells
15. A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing
16. Involvement of U6 snRNA in 5′ splice site selection
17. The mutational spectrum of single-pair substitutions in mRNA splice junctions of human genes: Causes and consequences
18. Mutations in U6 snRNA that alter splice site specificity: Implications for the active site
19. Suppressor U1 snRNAs in Drosophila
20. Functional recognition of the 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
21. Mechanism for cryptic splice site activation during pre-mRNA splicing
22. Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage
23. U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites
24. Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro
25. A point mutation in the conserved hexanucleotide at a yeast 5′ splice site uncouples recognition, cleavage and ligation
26. mRNA splicing efficiency in yeast and the contribution of non-conserved sequences
27. Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe
28. Both the polypyrimidine tract and the 3′ splice site function prior to the first step of splicing in fission yeast
29. Evidence for a base-pairing interaction between U6 small nuclear RNA and the 5′ splice site during the splicing reaction in yeast
30. Association of U6 snRNA with the 5′ splice site region of pre-mRNA in the spliceosome
31. A U1 snRNA: Pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site
32. Mutational analysis of the interactions between U1 small nuclear RNA and pre-mRNA of yeast
33. 5′ splice site selection in yeast: Genetic alterations in base-pairing with U1 reveal additional requirements
34. The U5 and U6 small nuclear RNAs as active site components of the spliceosome
35. Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae
36. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
37. Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome
38. A U1 small ribonucleoprotein particle with altered specificity induces alternative splicing of an adenovirus E1A mRNA precursor
39. Fission yeast gene structure and recognition
40. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation