Mutational analysis of U1 function in Schizosaccharomyces pombe: Pre- mRNAs differ in the extent and nature of their requirements for this snRNA in vivo

RNA

Volumn 2, Issue 5, 1996, Pages 404-418

  Alvarez, Consuelo J ^a   Romfo, Charles M ^a   Vanhoy, Roger W ^a,b   Porter, Gregory L ^a,c   Wise, Jo Ann ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

base pairing; secondary structure; splice site selection; U6 snRNA

Indexed keywords

MESSENGER RNA PRECURSOR;

ALLELE; ARTICLE; FUNGAL GENETICS; GENE DISRUPTION; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POINT MUTATION; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE;

BASE SEQUENCE; CDC2 PROTEIN KINASE; DNA PRIMERS; GENES, LETHAL; INTRONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; POINT MUTATION; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RIBONUCLEOPROTEIN, U4-U6 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA, FUNGAL; SCHIZOSACCHAROMYCES;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCES POMBE; SCHIZOSACCHAROMYCES POMBE;

EID: 0029959521     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Abovich N, Liao XC, Rosbash M. 1994. The yeast MUD2 protein: An interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes & Dev 5:843-854.
2. Althoff SM, Stevens SW, Wise JA. 1994. The Srp54 GTPase is essential for protein export in the fission yeast Schizosaccharomyces pombe. Mol Cell Biol 14:7839-7854.
3. Ares MJ, Weiser B. 1995. Rearrangements of snRNA structure during assembly and function of the spliceosome. Prog Nucleic Acid Res Mol Biol 50:131-159.
4. Barabino SML, Blencowe BJ, Ryder U, Sproat BS, Lamond A. 1990. Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly. Cell 63:293-302.
5. Bindereif A, Green MR. 1986. Ribonucleoprotein complex formation during pre-mRNA splicing in vitro. Mol Cell Biol 6:2582-2592.
6. Black DL, Chabot B, Steitz JA. 1985. U2 as well as U1 small nuclear ribonucleoproteins are involved in pre-messenger RNA splicing. Cell 42:737-750.
7. Brennwald P, Liao X, Holm K, Porter G, Wise JA. 1988. U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals. Mol Cell Biol 8:1580-1590.
8. Champion-Arnaud P, Gozani O, Palandijian L, Reed R. 1995. Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch site mutations. Mol Cell Biol 15:5750-5756.
9. Cohen JB, Broz SD, Levinson AD. 1993. U1 small nuclear RNAs with altered specificity can be stably expressed in mammalian cells and promote permanent changes in pre-mRNA splicing. Mol Cell Biol 13:2666-2676.
10. Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. 1993. Mutations in the conserved loop of human U5 snRNA generate the use of novel cryptic 5′ splice sites in vivo. EMBO J 12:5191-5200.
11. Crispino JD, Blencowe BJ, Sharp PA. 1994. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 265:1866-1869.
12. Crispino JD, Sharp PA. 1995. A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1 independent splicing. Genes & Dev 9:2314-2323.
13. Durkacz B, Carr A, Nurse P. 1986. Transcription of the cdc2 cell cycle control gene of the fission yeast Schizosaccharomyces pombe. EMBO J 5:369-373.
14. Eng FJ, Warner JR. 1991. Structural basis for the regulation of splicing of a yeast messenger RNA. Cell 65:797-804.
15. Frendewey D, Barta I, Gillespie M, Potashkin J. 1990. Schizosaccharomyces U6 genes have a sequence within their introns that matches the B box consensus of tRNA internal promoters. Nucleic Acids Res 18:2025-2032.
16. Fu XD. 1995. The superfamily of arginine/serine-rich splicing factors. RNA 1:663-680.
17. Goguel V, Liao X, Rymond BC, Rosbash M. 1991. U1 snRNP can influence 3′-splice site selection as well as 5′-splice site selection. Genes & Dev 5:1430-1438.
18. Goguel V, Yang Y, Rosbash M. 1993. Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing. Mol Cell Biol 13:6881-6848.