Coupling between replication and packaging of flavivirus RNA: Evidence derived from the use of DNA-based full-length CDNA clones of kunjin virus

Journal of Virology

Volumn 75, Issue 10, 2001, Pages 4633-4640

  Khromykh, A A ^a   Varnavski, A N ^a   Sedlak, P L ^a   Westaway, E G ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA; GENE PRODUCT; RNA DIRECTED RNA POLYMERASE; STRUCTURAL PROTEIN; VIRUS RNA;

ARTICLE; DNA PACKAGING; FLAVIVIRUS; GENE AMPLIFICATION; GENE CONSTRUCT; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; KUNJIN VIRUS; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN MOTIF; RNA REPLICATION; RNA TRANSCRIPTION; RNA TRANSLATION; VIRION; VIRUS REPLICATION;

ANIMALS; CELL LINE; CRICETINAE; DNA, VIRAL; ENCEPHALITIS VIRUSES, JAPANESE; RNA, VIRAL; VIRAL CORE PROTEINS; VIRAL ENVELOPE PROTEINS; VIRION; VIRUS ASSEMBLY; VIRUS REPLICATION;

EID: 0035032324     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.10.4633-4640.2001     Document Type: Article

References (22)

1. The replication of picornavaruses
2. Nucleotide and complete amino acid sequences of Kunjin virus: Definitive gene order and characteristics of the virus-specified proteins
3. Reversible dissociation of the poliovirus replication complex: Functions and interactions of its components in viral RNA synthesis
4. Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA
5. Subgenomic replicons of the flavivirus Kunjin: Construction and applications
6. Encapsidation of the flavivirus Kunjin replicon RNA by using a complementation system providing Kunjin structural proteins in trans
7. Trans-complementation of flavivirus RNA polymerase gene NS5 by using Kunjin virus replicon-expressing BHK cells
8. Trans-complementation analysis of flavivirus Kunjin NS5 gene reveals an essential role for translation of its N-terminal half in RNA replication
9. Efficient trans-complementation of the flavivirus Kunjin NS5 protein but not of the NS1 protein requires its coexpression with other components of the viral replicase
10. Cis- and trans-acting elements in Flavivirus RNA replication
11. Trans-complementation of yellow fever virus NS1 reveals a role in early RNA replication
12. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function
13. Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A
14. Markers for trans-Golgi membranes and the intermediate compartment localize to induced membranes with distinct replication functions in Flavivirus-infected cells
15. Functional coupling between replication and packaging of poliovirus replicon RNA
16. Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes
17. Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin replicon vectors
18. Replication of flaviviruses
19. Flavivirus replication strategy
20. Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
21. Ultrastructure of Kunjin virus-infected cells: Colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures
22. Nascent flavivirus RNA co-localized in situ with double-stranded RNA in stable replication complexes