Molecular cloning of a gene (polA) coding for an unusual DNA polymerase I from Treponema pallidum

Journal of Medical Microbiology

Volumn 49, Issue 7, 2000, Pages 657-667

  Rodes, Berta ^a,b   Liu, Hsi ^a   Johnson, Steven ^a   George, Robert ^a   Steiner, Bret ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b HOSPITAL UNIVERSITARIO LA PAZ   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ANTIBODY; BACTERIAL DNA; BACTERIAL PROTEIN; CYSTEINE; DNA DIRECTED DNA POLYMERASE BETA; EXONUCLEASE; SINGLE STRANDED DNA; SYNTHETIC PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CLONING VECTOR; CONTROLLED STUDY; DNA SYNTHESIS; ESCHERICHIA COLI; GENE INSERTION; GENETIC CODE; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; START CODON; TREPONEMA PALLIDUM; WESTERN BLOTTING;

TREPONEMA PALLIDUM;

EID: 0033922804     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-49-7-657     Document Type: Article

References (34)

1. 1. Cox DL, Riley B, Chang P, Sayahtaheri P, Tassell S, Hevelone J. Effects of molecular oxygen, oxidation-reduction potential, and antioxidants upon in vitro replication of Treponema pallidum subsp. pallidum. Appl Environ Microbiol 1990; 56: 3063-3072.
2. 2. Steiner B, Wong GHW, Graves S. Susceptibility of Treponema pallidum to the toxic products of oxygen reduction and the non-treponemal nature of its catalase. Br J Vener Dis 1984; 60: 14-22.
3. 3. Fitzgerald T. In vitro cultivation of Treponema pallidum: a review. Bull World Health Organ 1981; 33: 787-812.
4. 4. Austin FE, Barbieri JT, Corin RE, Grigas KE, Cox CD. Distribution of superoxide dismutase, catalase, and peroxidase activities among Treponema pallidum and other spirochaetes. Infect Immun 1981; 33: 372-379.
5. 5. Imlay JA, Linn S. DNA damage and oxygen radical toxicity. Science 1988; 240: 1302-1309.
6. 6. Steiner BM, Wong GHW, Sutrave P, Graves S. Oxygen toxicity in Treponema pallidum: deoxyribonucleic acid single-stranded breakage induced by low doses of hydrogen peroxide. Can J Microbiol 1984; 30: 1467-1476.
7. 7. Friedberg EC, Walker GC, Siede W. DNA repair and mutagenesis. Washington, DC, ASM Press. 1995.
8. 8. Cumberland MC, Turner TB. The rate of multiplication of Treponema pallidum in normal and immune rabbits. Am J Syph Gonor Ven Dis 1949; 33: 201-212.
9. 9. Demple B, Harrison L. Repair of oxidative damage to DNA: enzymology and biology. Annu Rev Biochem 1994; 63: 915-948.
10. 10. Krasin F, Hutchinson F. Repair of DNA double-strand breaks in Escherichia coli, which require recA function and the presence of a duplicate geonome. J Mol Biol 1977; 116: 81-98.
11. 11. Fraser CM, Norris SJ, Weinstock GM et al. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 1998; 281: 375-388.
12. 12. Yanisch-Perron C, Vieira J Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985; 33: 103-119.
13. 13. Shyamala V, Ames GF. Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR. Gene. 1989; 84: 1-8.
14. 14. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74: 5463-5467.
15. 15. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory. 1989.
16. 16. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 1979; 76: 4350-4354.
17. 17. Kingsbury DT, Helinski DR. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun 1970; 41: 1538-1544.
18. 18. Johnson SR. Cloning of the replication region of pGR9091, an R. factor of Neisseria gonorrhoeae. In: Schoolnik GK, Brooks GF, Falkow S et al. (eds) The pathogenic neisseriae. Washington, DC, ASM. 1985: 222-227.
19. 19. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132: 6-13.
20. 20. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalities and weight matrix choice. Nucleic Acids Res 1994; 22: 4673-4680.
21. 21. Felsenstein J. In PHYLIP (Phylogeny Inference Package), version 3.5c. Department of Genetics, University of Washington, Seattle. 1992
22. 22. Fleischmann RD, Adams MD, White O et al. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 1995; 269: 496-512.
23. 23. Vellanoweth RL, Rabinowitz JC. The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilus and Escherichia coli in vivo. Mol Microbiol 1992; 6: 1105-1114.
24. 24. Kornberg A, Baker TA. DNA replication, 2nd edn. New York, WH Freeman, 1992.
25. 25. Blanco L, Bernad A, Blasco MA, Salas M. A general structure for DNA-dependent DNA polymerases. Gene 1991; 100: 27-38.
26. 26. Huberts P. Mizrahi V. Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis. Gene 1995; 164: 133-136.
27. 27. Lawyer FC, Stoffel S, Saiki RK, Myambo K, Drummond R, Gelfand DH. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J Biol Chem 1989; 264: 6427-6437.
28. 28. Fahey RC, Hunt JS, Windham GC. On the cysteine and cystine content of proteins. Difference between intracellular and extracellular proteins. J Mol Evol 1977; 10: 155-160.
29. 29. Stephens RS, Kalman S, Lammel C et al. Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 1998; 282: 754-759.
30. 30. Storch TG, Talley GD. Oxygen concentration regulates the proliferative response of human fibroblasts to serum and growth factors. Exp Cell Res 1988; 175: 317-325.
31. 31. Fraser CM, Casjens S, Huang WM et al. Genomic sequence of a Lyme disease spirochete, Borrelia burgdorferi. Nature 1997; 390: 580-586.
32. 32. Huang Y-P, Downie JA, Ito J. Primary structure of the DNA polymerase I gene of an α-proteobacterium, Rhizobium leguminosarum, and comparison with other family A DNA polymerases. Curr Microbiol 1999; 38: 355-359.
33. 33. Fsihi H, Cole ST. The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability. Mol Microbiol 1995; 16: 909-919.
34. 34. Gordhan BG, Mizrahi V. The RLEP-flanked polA gene from Mycobacterium leprae is not transcribed in Microbacterium smegmatis. Gene 1997; 187: 63-66.