Detection of Treponema pallidum by a sensitive reverse transcriptase PCR

Journal of Clinical Microbiology

Volumn 35, Issue 6, 1997, Pages 1348-1352

  Centurion Lara, Arturo ^a   Castro, Christa ^a   Shaffer, Jeanne M ^a   Van Voorhis, Wesley C ^a   Marra, Christina M ^a   Lukehart, Sheila A ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AQUEOUS HUMOR; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD; CEREBROSPINAL FLUID; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERODIAGNOSIS; SYPHILIS; TREPONEMA PALLIDUM;

BACTERIA (MICROORGANISMS); ORYCTOLAGUS CUNICULUS; TREPONEMA PALLIDUM;

EID: 0030922325     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.6.1348-1352.1997     Document Type: Article

References (19)

1. Burstain, J. M., E. Grimprel, S. A. Lukehart, M. V. Norgard, and J. D. Radolf. 1991. Sensitive detection of Treponema pallidum by using the polymerase chain reaction. J. Clin. Microbiol. 29:62-69.
2. Centurion-Lara, A., C. Castro, W. C. Van Voorhis, and S. A. Lukehart. 1996. Two 16S-23S ribosomal DNA intergenic regions in different Treponema pallidum subspecies contain tRNA genes. FEMS Microbiol. Lett. 143:235-240.
3. Gordon, S. M., M. E. Eaton, R. George, S. Larsen, S. A. Lukehart, J. Kuypers, C. M. Marra, and S. Thompson. 1994. The response of symptomatic neurosyphilis to high-dose intravenous penicillin G in patients with human immunodeficiency virus infection. N. Engl. J. Med. 331:1469-1473.
4. Grimprel, E., P. J. Sanchez, G. D. Wendel, J. M. Burstain, G. H. McCracken, J. D. Radolf, and M. V. Norgard. 1991. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid. J. Clin. Microbiol. 29:1711-1718.
5. Hay, P. E., J. R. Clarke, R. A. Strugnell, D. Taylor-Robinson, and D. Goldmeier. 1990. Use of polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid. FEMS Microbiol. Lett. 68:233-238.
6. Hay, P. E., D. Taylor-Robinson, and D. Goldmeier. 1990. Detection of treponemal DNA in the CSF of patients with syphilis and HIV infection using the polymerase chain reaction. Genitourin. Med. 66:428-432.
7. Haydock, P. Personal communication.
8. Horowitz, H. W., M. P. Valsamis, V. Wicher, F. Abbruscato, S. A. Larsen, G. P. Wormser, and K. Wicher. 1994. Brief report: cerebrospinal syphilitic gumma confirmed by the polymerase chain reaction in a man with human immunodeficiency virus infection. N. Engl. J. Med. 331:1488-1491.
9. Jethwa, H. S., J. L. Schmitz, G. Dallabetta, F. Behets, I. Hoffman, H. Hamilton, G. Lule, M. Cohen, and J. D. Folds. 1995. Comparison of molecular and microscopic techniques for detection of Treponema pallidum in genital ulcers. J. Clin. Microbiol. 33:180-183.
10. Lukehart, S. A., E. W. Hook III, S. A. Baker-Zander, A. C. Collier, C. A. Critchlow, and H. H. Handsfield. 1988. Invasion of the central nervous system by Treponema pallidum: implications for diagnosis and treatment. Ann. Intern. Med. 109:855-862.
11. Marra, C. M. 1995. Neurosyphilis: a guide for clinicians. Neurologist 1:157-166.
12. Noordhoek, G. T., E. C. Wolters, M. E. J. de Jonge, and J. D. A. van Embden. 1991. Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment. J. Clin. Microbiol. 29:1976-1984.
13. Orle, K. A., C. A. Gates, D. H. Martin, B. A. Body, and J. B. Weiss. 1996. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. J. Clin. Microbiol. 34:49-54
14. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
15. Sanchez, P. J., G. D. Wendel, E. Grimprel, M. Goldberg, M. Hall, O. Arencibia-Mireles, J. D. Radolf, and M. V. Norgard. 1993. Evaluation of molecular methodologies and rabbit infectivity testing for diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum. J. Infect. Dis. 167:148-157.
16. Turner, T. B., P. H. Hardy, and B. Newman. 1969. Infectivity tests in syphilis. Br. J. Vener. Dis. 45:183-196.
17. Wicher, K., G. T. Noordhoek, F. Abbruscato, and V. Wicher. 1992. Detection of Treponema pallidum in early syphilis by DNA amplification. J. Clin. Microbiol. 30:497-500.
18. Wicher, V., F. Abbruscato, and K. Wicher. 1996. Persistence of virulent (V) and heat-killed (HK) Treponema pallidum (Tp), in rabbit (Ra) and guinea pig (Gp) as determined by PCR, abstr. D-51, p. 250. In Abstracts of the 96th General Meeting of the American Society for Microbiology 1996. American Society for Microbiology, Washington, D.C.
19. Wu, W., and B. Dai. 1994. 16S rRNA reverse transcription-polymerase chain reaction of leptospires. J. West China Univ. Med. Sci. 25:166-169.