Rapid stiffening of integrin receptor-actin linkages in endothelial cells stimulated with thrombin: A magnetic bead microrheology study

Biophysical Journal

Volumn 80, Issue 6, 2001, Pages 2649-2657

  Bausch, Andreas R ^a   Hellerer, Ulrike ^a   Essler, Markus ^b   Aepfelbacher, Martin ^b   Sackmann, Erich ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ESCHERICHIA COLI ENDOTOXIN; FIBRONECTIN; INTEGRIN RECEPTOR; MYOSIN LIGHT CHAIN KINASE; PROTEIN KINASE (CALCIUM,CALMODULIN); RHO KINASE; THROMBIN; TOXIN;

ARTICLE; BLOOD RHEOLOGY; CELL MEMBRANE; CELL SURFACE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVATION; HUMAN; HUMAN CELL; MAGNETISM; MATHEMATICAL MODEL; MEMBRANE STABILIZATION; PROTEIN PROTEIN INTERACTION; VISCOELASTICITY; YOUNG MODULUS;

ESCHERICHIA COLI;

EID: 0034995558     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)76234-0     Document Type: Article

References (22)

1. Aepfelbacher, M., M. Essler, E. Huber, A. Czech, and P. C. Weber. 1996. Rho is a negative regulator of human monocyte spreading. J. Immunol. 157:5070-5075.
2. Allen, W. E., G. E. Jones, J. W. Pollard, and A. J. Ridley. 1997. Rho, Rac and Cdc42 regulate actin organization and cell adhesion in macrophages. J. Cell Sci. 110:707-720.
3. Ashkin, A. 1997. Optical trapping and manipulation of neutral particles using lasers. Proc. Natl. Acad. Sci. USA. 94:4853-4860.
4. Bausch, A. R., W. Möller, and E. Sackmann. 1999. Measurement of local viscoelasticity and forces in living cells by magnetic tweezers. Biophys. J. 76:573-579.
5. Bausch, A. R., F. Ziemann, A. A. Boulbitch, K. Jacobson, and E. Sackmann. 1998. Local measurements of viscoelastic parameters of adherent cell surfaces by magnetic bead microrheometry. Biophys. J. 75: 2038-2049.
6. Bodmer, J. E., J. Van Engelenhoven, G. Reyes, K. Blackwell, A. Kamath, D. M. Shasby, and A. B. Moy. 1997. Isometric tension of cultured endothelial cells: new technical aspects. Microvasc. Res. 53:261-271.
7. Chicurel, M. E., R. H. Singer, C. J. Meyer, and D. E. Ingber. 1998. Integrin binding and mechanical tension induce movement of mRNA and ribosomes to focal adhesions. Nature. 392:730-733.
8. Essler, M., M. Amano, H. J. Kruse, K. Kaibuchi, P. C. Weber, and M. Aepfelbacher. 1998. Thrombin inactivates myosin light chain phosphatase via Rho and its target Rho kinase in human endothelial cells. J. Biol. Chem. 273:21867-21874.
9. Essler, M., M. Retzer, M. Bauer, J. W. Heemskerk, M. Aepfelbacher, and W. Siess. 1999. Mildly oxidized low density lipoprotein induces contraction of human endothelial cells through activation of Rho/Rho kinase and inhibition of myosin light chain phosphatase. J. Biol. Chem. 274: 30361-30364.
10. Garcia, J. G., and K. L. Schaphorst. 1995. Regulation of endothelial cell gap formation and paracellular permeability. J. Invest. Med. 43:117-126.
11. Hartwig, J. H., G. M. Bokoch, C. L. Carpenter, P. A. Janmey, L. A. Taylor, A. Toker, and T. P. Stossel. 1995. Thrombin receptor ligation and activated Rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets. Cell. 82:643-653.
12. Higgs, H. N., and T. D. Pollard. 1999. Regulation of actin polymerization by Arp2/3 complex and WASP/Scar proteins. J. Biol. Chem. 274: 32531-32534.
13. Jalink, K., E. J. van Corven, T. Hengeveld, N. Morii, S. Narumiya, and W. H. Moolenaar. 1994. Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho. J. Cell Biol. 126:801-810.
14. Kolodney, M. S., and R. B. Wysolmerski. 1992. Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study. J. Cell Biol. 117:73-82.
15. Lampugnani, M. G., M. Corada, L. Caveda, F. Breviario, O. Ayalon, B. Geiger, and E. Dejana. 1995. The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin). J. Cell Biol. 129:203-217.
16. Lerm, M., J. Selzer, A. Hoffmeyer, U. R. Rapp, K. Aktories, and G. Schmidt. 1999. Deamidation of Cdc42 and Rac by Escherichia coli cytotoxic necrotizing factor 1: activation of c-Jun N-terminal kinase in HeLa cells. Infect. Immun. 67:496-503.
17. Miyamoto, S., S. K. Akiyama, and K. M. Yamada. 1995. Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function. Science. 267:883-885.
18. Miyamoto, S., B. Z. Katz, R. M. Lafrenie, and K. M. Yamada. 1998. Fibronectin and integrins in cell adhesion, signaling, and morphogenesis. Ann. N.Y. Acad. Sci. 857:119-129.
19. Raucher, D., T. Stauffer, W. Chen, K. Shen, S. L. Guo, J. D. York, M. P. Sheetz, and T. Meyer. 2000. Phosphalidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell. 100:221-228.
20. Schmidt, G., P. Sehr, M. Wilm, J. Selzer, M. Mann, and K. Aktories. 1997. Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature. 387:725-729.
21. Yamada, K. M., and B. Geiger. 1997. Molecular interactions in cell adhesion complexes. Curr. Opin. Cell Biol. 9:76-85.
22. Ziemann, F., J. Rädler, and E. Sackmann. 1994. Local measurements of viscoelastic moduli of entangled actin networks using an oscillating magnetic bead micro-rheometer. Biophys. J. 66:2210-2216.