Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors

Molecular Therapy

Volumn 3, Issue 4, 2001, Pages 623-630

  Hughes, C ^a   Galea Lauri, J ^a   Farzaneh, F ^a   Darling, D ^a  

^a ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

Biotin; Concentration; Fibronectin; Gene therapy; Lectin; Paramagnetic; Retrovirus; Streptavidin; Targeting

Indexed keywords

ANTIBODY; BIOTIN; CELL SURFACE PROTEIN; FIBRONECTIN; LECTIN; STREPTAVIDIN; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BIOTINYLATION; BONE MARROW CELL; COLONY FORMING UNIT; CONCENTRATION (PARAMETERS); CONJUGATION; CONTROLLED STUDY; DNA PACKAGING; GENE TARGETING; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MAGNETIC FIELD; MOUSE; NONHUMAN; RETROVIRUS; RETROVIRUS INFECTION; VIRUS INFECTIVITY; VIRUS TITRATION;

ANIMALIA; MURINAE; UNIDENTIFIED RETROVIRUS;

EID: 0034990563     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2001.0268     Document Type: Article

References (40)

1. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus
2. Low-speed centrifugation of retroviral vectors absorbed to a particulate substrate: A highly effective means of enhancing retroviral titre
3. Nucleotide sequence of the gene for fibronectin-binding protein from Staphylococcus aureus: Use of this peptide sequence in the synthesis of biologically active peptides
4. Two different genes encode fibronectin binding proteins in Staphylococcus aureus: The complete nucleotide sequence and characterization of the second gene
5. Reduction in fibronectin expression and alteration in cell morphology are coincident in NIH3T3 cells expressing a mutant E2F1 transcription factor
6. Green fluorescent protein as a selectable marker of fibronectin-facilitated retroviral gene transfer in primary human T lymphocytes
7. Colocalization of retrovirus and target cells on specific fibronectin fragments increases genetic transduction of mammalian cells
8. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production
9. Construction and use of a safe and efficient amphotropic packaging cell line
10. A safe packaging line for gene transfer: Separating viral genes on two different plasmids
11. High-titer packaging cells producing recombinant retroviruses resistant to human serum
12. Type C retrovirus inactivation by human complement is determined by both viral genome and the producer cell
13. Sensitization of cells and retroviruses to human serum by (α1-3) galactosyltransferase
14. In vitro immune modulation by antibodies coupled to tumour cells
15. Alternate pathways of secretion of simian immunodeficiency virus envelope glycoproteins
16. Quantitation of MoMuLV envelope protein on the cell surface
17. Cloning techniques
18. Retroviral infection is limited by Brownian motion
19. Analysis of 4070A envelope levels in retroviral preparations and effect on target cell transduction efficiency
20. Gene sequences suggest the inactivation of α-1,3-galactosyltransferase in catarrhines after the divergence of apes from monkeys
21. Frameshift and nonsense mutations in a genomic sequence homologous to a murine UDP-Gal:β-D-Gal(1,4)-D-GlcNAc α(1,3)-galactosyltransferase cDNA
22. 4, isolated from Bandeirea simplicifolia
23. Protein-carbohydrate interaction. II. Inhibition studies on the interaction of concanavalin A with polysaccharides
24. Improved titres of retroviral vectors from the human FLYRD18 packaging cell line in serum- and protein-free medium
25. Inhibitory effects of novel mannose-binding lectins on HIV-infectivity and syncytium formation
26. Screening for inhibitors of HIV gp120-CD4 binding using an enzyme linked immunosorbent assay
27. Concanavalin A-immobilized polystyrene nanospheres capture HIV-1 virions and gp120: Potential approach toward prevention of viral transmission
28. Photoactivatable retroviral vectors: A strategy for targeted gene delivery
29. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to a very high titre and efficient gene transfer into mammalian and non-mammalian cells
30. Breeding of retroviruses by DNA shuffling for improved stability and processing yields
31. Retroviral vectors preloaded with a viral receptor-ligand bridge protein are targeted to specific cell types
32. Targeting retroviral infection to vascular lesions by genetic engineering of the MoMLV gp70 envelope protein
33. Retroviral vector targeting human cells via c-Kit-stem cells factor interaction
34. Identification of the block in targeted retroviral-mediated gene transfer
35. Targeting retroviral vectors to CD34-expressing cells: Binding to CD34 does not catalyze virus-cell fusion
36. Targeting human T cells by retroviral vectors displaying antibody domains selected from a phage display library
37. Targeting strategy for gene delivery to carcinoembryonic antigen-producing cancer cells by retrovirus displaying single-chain variable fragment antibody
38. Retroviral vector targeting to melanoma cells by single-chain antibody incorporation in envelope
39. Cationic liposomes enhance the rate of transduction by a recombinant retroviral vector in vitro and in vivo
40. A stable system for the high-titre production of multiply attenuated lentiviral vectors