Structure of Cry2Aa suggests an unexpected receptor binding epitope

Structure

Volumn 9, Issue 5, 2001, Pages 409-417

  Morse, R J ^a   Yamamoto, T ^b,c   Stroud, R M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Sandoz Agro   (United States)

^c Maxygen   (United States)

Author keywords

Bacillus thuringiensis; Binding epitope; Cry2Aa; Crystal structure; Delta endotoxin; X ray

Indexed keywords

ASPARAGINE; CRY2AA PROTOXIN; CYSTEINE; EPITOPE; GLYCINE; HISTIDINE; ISOLEUCINE; LEUCINE; PROTOXIN; SERINE; THREONINE; TOXIN; UNCLASSIFIED DRUG; VALINE;

ARTICLE; BACILLUS THURINGIENSIS; CRYSTAL STRUCTURE; FLY; LARVA; LEPIDOPTERA; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RECEPTOR BINDING; SPECIES DIFFERENCE; STRUCTURE ANALYSIS; TARGET ORGAN; TISSUE SPECIFICITY; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ENDOTOXINS; HEMOLYSIN PROTEINS; INSECT PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, CELL SURFACE;

BACILLUS THURINGIENSIS; DIPTERA; HEXAPODA; INSECTA; LEPIDOPTERA; PAPILIONOIDEA;

EID: 0034980642     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00601-3     Document Type: Article

References (51)

1. Bacillus thuringiensis and its pesticidal crystal proteins
2. Dissolution and degradation of Bacillus thuringiensis δ-endotoxin by gut juice protease of the silkworm Bombyx mori
3. The solubility of inclusion proteins from Bacillus thuringiensis is dependent upon protoxin composition and is a factor in toxicity to insects
4. Unusual proteolysis of the protoxin and toxin from Bacillus thuringiensis. Structural implications
5. Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts
6. Interaction of Bacillus thuringiensis with membrane vesicles isolated from lepidopteran larval midgut
7. Specificity of Bacillus thuringiensis δ-endotoxins: Importance of specific receptors on the brush border membrane of the mid-gut of target insects
8. Delta-endotoxins form cation-selective channels in planar lipid bilayers
9. 2O from phospholipid vesicles is catalyzed by reconstituted midgut membrane
10. Lepidopteran-specific crystal toxins from Bacillus thuringiensis form cation- and anion-selective channels in planar lipid bilayers
11. Mechanism of inhibition of active potassium transport in isolated midgut of Manduca sexta by Bacillus thuringiensis endotoxin
12. Transport physiology of lepidopteran midgut in relation to the action of B.t. delta-endotoxin
13. Colloid-osmotic lysis is a general feature of the mechanism of Bacillus thuringiensis d-endotoxins with different insect specificity
14. V-ATPase energized epithelia and biological insect control
15. Revision of the nomenclature of the Bacillus thuringiensis pesticidal crystal proteins
16. Transgenic pollen harms monarch larvae
17. Mechanism of insect resistance to the microbial insecticide Bacillus thuringiensis
18. Bt resistance management
19. Isolation of a protein from the parasporal crystal of Bacillus thuringiensis var. kurstaki toxic to the mosquito larva Aedes taeniarhynehus
20. Amino acid sequence and entomocidal activity of the P2 crystal protein. An insect toxin from Bacillus thuringiensis var. kurstaki
21. Bacillus thuringiensis crystal proteins: Recent advances in understanding its insecticidal activity
22. Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects
23. Mode of action of CryIIA: A Bacillus thuringiensis delta-endotoxin
24. Location of the dipteran specificity region in a lepidopteran-dipteran crystal protein from Bacillus thuringiensis
25. Location of a lepidopteran specificity region in insecticidal crystal protein CryIIA from Bacillus thuringiensis
26. Two highly related insecticidal crystal proteins of Bacillus thuringiensis subsp. kurstaki possess different host range specificities
27. Production of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering
28. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 Å resolution
29. Bacillus thuringiensis CryIA(a) insecticidal toxin: Crystal structure and channel formation
30. Domain III exchanges of Bacillus thuringiensis Cry1A toxins affect binding to different gypsy moth midgut receptors
31. Domain III substitution in Bacillus thuringiensis delta-endotoxin CryIA(b) results in superior toxicity for Spodoptera exigua and altered membrane protein recognition
32. Single-site mutations in the conserved alternating-arginine region affect ionic channels formed by CryIAa, a Bacillus thuringiensis toxin
33. Membrane-permeabilizing activities of Bacillus thuringiensis coleopteran-active toxin CryIIIB2 and CryIIIB2 domain I peptide
34. Antibody framework residues affecting the conformation of the hypervariable loops
35. Structural insights into the evolution of an antibody combining site
36. Inheritance of resistance to Bacillus thuringiensis toxin (Dipel ES) in the European corn borer
37. Prey-mediated effects of Cry1Ab toxin and protoxin and Cry2A protoxin on the predator Chrysoperla carnea
38. Insecticidal toxins from the bacterium Photorhabdus luminescens
39. Insecticidal activity of the protein encoded by the cryV gene of Bacillus thuringiensis kurstaki INA-02
40. Identification of entomocidal toxins of Bacillus thuringiensis by high-performance liquid chromatography
41. Processing of X-ray diffraction data collected in oscillation mode
42. PHASES-95: A program package for the processing and analysis of diffraction data from macromolecules
43. Methods used in the structure determination of bovine mitochondrial F-1 Atpase
44. Improved methods for building protein models in electron density maps and the location of errors in these models
45. Template convolution to enhance or detect structural features in macromolecular electron-density maps
46. The MIDAS display system
47. Satisfying hydrogen bonding potential in proteins
48. ALSCRIPT: A tool to format multiple sequence alignments