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Volumn 280, Issue 5372, 1998, Pages 2129-2132

Insecticidal toxins from the bacterium photorhabdus luminescens

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN;

EID: 0032568883     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5372.2129     Document Type: Review
Times cited : (374)

References (35)
  • 5
    • 3542995313 scopus 로고
    • thesis, University of Wisconsin-Madison
    • D. J. Bowen, thesis, University of Wisconsin-Madison (1995).
    • (1995)
    • Bowen, D.J.1
  • 16
    • 3542992889 scopus 로고    scopus 로고
    • note
    • 4, pH 6.9). Toxic fractions were concentrated, equilibrated with 10 mM tris-HCI (pH 8.6), and loaded onto a weak anion-exchange HPLC column (301VHP575) (Vydac) and eluted with a KCl gradient.
  • 17
    • 3542995908 scopus 로고    scopus 로고
    • note
    • 2 cubes of artificial diet (Gypsy moth wheat germ diet; ICN Biomedical). For the range of other insects assayed (Table 1) either a mixture of the high molecular weight toxin complexes was added to the diet as for M. sexta or injectable activity was measured by injection of toxin in buffer directly into the insect hemocoel. For bioassay of the to gene disruptions, we used the same procedure except that instead of applying the same weight of purified Tc proteins, we added the same volume of different concentrations of culture broth to the diet cubes.
  • 19
    • 3543007383 scopus 로고    scopus 로고
    • note
    • Antisera were raised against the high molecular weight toxin fraction before further separation by HPLC. A rabbit polyclonal antiserum was raised against the native toxin, and a monoclonal antibody (C5F2) was derived from mice immunized with heat-denatured toxin.
  • 20
    • 3543046971 scopus 로고    scopus 로고
    • note
    • + (Stratagene) from size-fractionated DNA partially digested with Sau 3a and transformed into competent library efficiency XL2-Blue MRF' E. coli (Stratagene). The library was screened with both the monoclonal and the polyclonal antitoxin antibodies. Immunoreactive clones were restriction mapped and sequenced with Sequenase 2.0 (United States Biochemical).
  • 22
    • 3543009826 scopus 로고    scopus 로고
    • personal communication
    • D. Guiney, personal communication.
    • Guiney, D.1
  • 23
    • 3543025359 scopus 로고    scopus 로고
    • World Intellectual Property, Patent WO 97/17432 (1997)
    • J. Ensign et al., World Intellectual Property, Patent WO 97/17432 (1997).
    • Ensign, J.1
  • 32
    • 3543029461 scopus 로고    scopus 로고
    • note
    • For examination of gut pathology, we fed Tca toxin to neonate M. sexta for 3 hours and to first-instar larvae for 48 hours. Larvae were fixed in Bouin's fluid overnight at 4°C, dehydrated, and embedded in paraffin wax; 6-μm paraffin sections were stained with Weigert's hematoxylin followed by Cason's trichome stain; then they were examined by light microscopy.
  • 33
    • 3543000124 scopus 로고    scopus 로고
    • note
    • Cells were spun down at 10,000g and then supernatants were concentrated in Millipore Centrifugal filtration units (Ultrafree, Biomax-100K) or diluted with growth medium.
  • 34
    • 3542997691 scopus 로고    scopus 로고
    • note
    • Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 35
    • 3543003755 scopus 로고    scopus 로고
    • note
    • We thank all at Dow AgroSciences Biotechnology for their encouragement and support of this project. Supported by Hatch funds, The Applied Research and Technology Fund, and The Industrial and Economic Development Fund, all administered by the University of Wisconsin-Madison and by DowAgrosciences.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.