Single-site mutations in the conserved alternating-arginine region affect ionic channels formed by CryIAa, a Bacillus thuringiensis toxin

Applied and Environmental Microbiology

Volumn 63, Issue 10, 1997, Pages 3978-3984

  Schwartz, J L ^a,c   Potvin, L ^a   Chen, X J ^b   Brousseau, R ^a   Laprade, R ^a   Dean, D H ^b  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b OHIO STATE UNIVERSITY   (United States)

^c NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; GLUTAMIC ACID; GLUTAMINE; HISTIDINE; LYSINE;

ARTICLE; BACILLUS THURINGIENSIS; CATION TRANSPORT; LIPID BILAYER; MEMBRANE PERMEABILITY; NONHUMAN; PHOSPHOLIPID MEMBRANE; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS;

BACILLUS THURINGIENSIS;

EID: 0030756318     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.10.3978-3984.1997     Document Type: Article

References (32)

1. Aronson, A. I., D. Wu, and C. Zhang. 1995. Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene. J. Bacteriol. 177: 4059-4075.
2. Bosch, D., B. Schipper, H. Van der Kleij, R. A. De Maagd, and W. J. Stiekema. 1994. Recombinant Bacillus thuringiensis crystal proteins with new properties: possibilities for resistance management. Bio/Technology 12:915-918.
3. Chen, X. J., M. K. Lee, and D. H. Dean. 1993. Site-directed mutations in a highly conserved region of Bacillus thuringiensis δ-endotoxin affect inhibition of short circuit current across Rombyx mori midguts. Proc. Natl. Acad. Sci. USA 90:9041-9045.
4. Colquhoun, D., and F. J. Sigworth. 1983. Fitting and statistical analysis of single-channel records, p. 191-263. In B. Sakmann and E. Neher (ed.), Single-channel recording. Plenum Press, New York, N.Y.
5. Convents, D., C. Houssier, I. Lasters, and M. Lauwereys. 1990. The Bacillus thuringiensis δ-endotoxin: evidence for a two domain structure of the minimal toxic fragment. J. Biol. Chem. 265:1369-1375.
6. English, L., H. L. Robbins, M. A. von Tersch, C. A. Kulesza, D. Ave, D. Coyle, C. S. Jany, and S. L. Slatin. 1994. Mode of action of CryIIA: a Bacillus thuringiensis delta-endotoxin. Insect Biochem. Mol. Biol. 24:1025-1035.
7. Fox, J. A. 1987. Ion channel subconductance states. J. Mcmbr. Biol. 97:1-8.
8. Ge, A. Z., D. Rivers, R. Milne, and D. H. Dean. 1991. Functional domains of Bacillus thuringiensis insecticidal crystal proteins. J. Biol. Chem. 266:17954-17958.
9. Geiser, M., S. Schweitzer, and C. Grimm. 1986. The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1. Gene 48:109-118.
10. Gill, S. S., E. A. Cowles, and P. V. Pietrantonio. 1992. The mode of action of Bacillus thuringiensis endotoxins. Annu. Rev. Entomol. 37:615-636.
11. Grochulski, P., L. Masson, S. Borisova, M. Pusztai-Carey, J. L. Schwartz, R. Brousseau, and M. Cygler M. 1995. Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. J. Mol. Biol. 254:447-464.
12. Haider, M. Z., and D. J. Ellar. 1989. Functional mapping of an entomocidal δ-endotoxin. Single amino acid changes produced by site-directed mutagenesis influence toxicity and specificity of the protein. J. Mol. Biol. 208:183-194.
13. Hille, B. 1992. Ionic channels of excitable membranes, 2nd ed., p. 315-336. Sinauer Associates Inc., Sunderland, Mass.
14. Höfte, H., and H. R. Whiteley. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53:242-255.
15. Knowles, B. H. 1994. Mechanism of action of Bacillus thuringiensis insecticidal δ-endotoxins. Adv. Insect Physiol. 24:275-308.
16. Knowles, B. H., and J. A. T. Dow. 1993. The crystal δ-endotoxins of Bacillus thuringiensis: models for their mechanism of action on the insect gut. Bioessays 15:469-176.
17. Laemmli, U. K., and M. Favre. 1973. Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol. 80:575-599.
18. Lee M. K., R. E. Milne, A. Z. Ge, and D. H. Dean. 1992. Domain III exchanges of Bacillus thuringiensis CryIA toxins affect binding to different gypsy moth midgut receptors. J. Biol. Chem. 267:3115-3121.
19. Lee, M. K., B. A. Young, and D. H. Dean. 1995. Location of a Bombyx mori receptor binding region on a Bacillus thuringiensis δ-endotoxin. Biochem. Biophys. Res. Commun. 216:306-312.
20. Li, J., J. Carroll, and D. J. Ellar. 1991. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 Å resolution. Nature 353:815-871.
21. Manivannan, K., S. V. Ramanan, R. T. Mathias, and P. R. Brink. 1992. Multichannel recordings from membranes which contain gap junction. Biophys. J. 61:216-227.
22. Masson, L., A. Mazza, L. Gringorten, D. Baines, V. Aneliunas, and R. Brousseau. 1994. Kinetics of Bacillus thuringiensis toxin binding with brush border membrane vesicles from susceptible and resistant larvae of Plutella xylostella. Mol. Microbiol. 14:851-860.
23. Monette, R., D. Savaria, L. Masson, R. Brousseau, and J. L. Schwartz. 1994. Calcium-activated potassium channels in the UCR-SE-1a lepidopteran cell line from the beet armyworm (Spodoptera exigua). J. Insect Physiol. 40:273-282.
24. Nishimoto, T., H. Yoshisue, K. Ihara, H. Sakai, and T. Komano. 1994. Functional analysis of block 5, one of the highly conserved amino acid sequences in the 130-kDa CryIVA protein produced by Bacillus thuringiensis subsp. israelensis. FEBS Lett. 348:249-254.
25. Schwartz, J. L., L. Garneau, L. Masson, and R. Brousseau. 1991. Early response of cultured lepidopteran cells to exposure to δ-endotoxin from Bacillus thuringiensis: involvement of calcium and anionic channels. Biochim. Biophys. Acta 1065:250-260.
26. Schwartz, J. L., L. Garneau, D. Savaria, L. Masson, and R. Brousseau. 1993. Lepidopteran-specific crystal toxins from Bacillus thuringiensis form cation- and anion-selective channels in planar lipid bilayers. J. Membr. Biol. 132: 53-62.
27. Schwartz, J. L., M. Juteau, P. Grochulski, M. Cygler, G. Préfontaine, R. Laprade, R. Brousseau, and L. Masson. 1997. Restriction of intramolecular movements within CrylAa toxin molecule of Bacillus thuringiensis by disulphide bond engineering. FEBS Lett. 410:397-402.
28. Schwartz, J. L., L. Potvin, J. Laflamme, A. Mazza, L. Masson, R. Brousseau, and R. Laprade. 1994. Effect of single site mutations on ionic channels formed by CryIA(c) Bacillus thuringiensis (B. thuringiensis) toxin. Biophys. J. 66:A221.
29. Slatin, S. L., C. K. Abrams, and L. English. 1990. Delta-endotoxins form cation-selective channels in planar lipid bilayers. Biochem. Biophys. Res. Commun. 169:765-772.
30. Von Tersch, M. A., S. L. Slatin, C. A. Kulesza, and L. H. English. 1994. Membrane-pcrmeabilizing activities of Bacillus thuringiensis coleopteran-active toxin CryIIIB2 and CryIIIB2 domain 1 peptide. Appl. Environ. Microbiol. 60:3711-3717.
31. Wolfersberger, M. G., X. J. Chen, and D. H. Dean. 1996. Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles. Appl. Environ. Microbiol. 62:279-282.
32. Wu, S. J., O. Alzate, B. J. Honoelle, W. J. Becktel, and D. H. Dean. Protein engineering of Bacillus thuringiensis δ-endotoxins: disulphide mutants in domain I of Cry3A increase stability with no loss of toxicity. Submilled for publication.