Ligand binding to transmembrane receptors on intact cells or membrane vesicles measured in a homogeneous 1-microliter assay format

Journal of Biomolecular Screening

Volumn 6, Issue 3, 2001, Pages 159-170

  Klumpp, Martin ^a   Scheel, Andreas ^a   Lopez Calle, Eloisa ^a   Busch, Michael ^a   Murray, Kenneth J ^b   Pope, Andrew J ^b  

^a EVOTEC BioSystems AG   (Germany)

^b SmithKline Beecham   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CXCR1; CHEMOKINE RECEPTOR CXCR2; INTERLEUKIN 8; MEMBRANE RECEPTOR;

ARTICLE; ASSAY; BINDING AFFINITY; FLUORESCENCE; INHIBITION KINETICS; LIGAND BINDING; MEMBRANE VESICLE; PRIORITY JOURNAL; SCREENING; SENSITIVITY AND SPECIFICITY;

ANIMALS; CELL MEMBRANE; CELL SURVIVAL; CELL-FREE SYSTEM; CHO CELLS; CRICETINAE; HETEROTRIMERIC GTP-BINDING PROTEINS; INTERLEUKIN-8; KINETICS; LIGANDS; LIPOSOMES; MINIATURIZATION; RECEPTORS, CELL SURFACE; RECEPTORS, INTERLEUKIN-8A; SENSITIVITY AND SPECIFICITY;

EID: 0034979380     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1089/108705701300362692     Document Type: Article

References (28)

1. High-throughput screening: Advances in robotics and miniturization continue to accelerate drug lead identification
2. Advances in the human genome project: A review
3. Orphan G protein-coupled receptors: A neglected opportunity for pioneer drug discovery
4. Fluorescence correlation spectroscopy-based screening technology
5. Single molecule detection technologies in miniaturized high throughput screening: Fluorescence correlation spectroscopy
6. Macroscopic versus microscopic fluorescence techniques in (ultra)-high-throughput screening
7. Studying receptor-ligand interactions with single molecule sensitivity
8. Single molecule detection technologies in miniaturized high throughput screening: Binding assays for G protein coupled receptors using fluorescence intensity distribution analysis and fluorescence anisotropy
9. Identification of a potent, selective non-peptide CXCR2 antagonist that inhibits interleukin-8-induced neutrophil migration
10. Molecular tinkering of G protein-coupled receptors: An evolutionary success
11. Opportunities for novel therapeutic agents acting at chemokine receptors
12. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology
13. Distribution of molecular aggregation by analysis of fluctuation moments
14. On the analysis of high order moments of fluorescence fluctuations
15. 50) of an enzymatic reaction
16. Molecular approaches to structure-function analysis of interleukin-8
17. Ligand-receptor binding measured by laser-scanning imaging
18. Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: Potential role of an analog of the retinal protein arrestin (48-kDa protein)
19. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization
20. Differences between natural and recombinant G protein-coupled receptor systems with varying receptor/G protein stoichiometry
21. Cellular autofluorescence - Is it due to flavins?
22. Autofluorescence of viable cultured mammalian cells
23. Reverse intrinsic activity of antagonists on G protein-coupled receptors
24. Chemokine receptor-ligand interactions measured using time-resolved fluorescence
25. A simple statistical parameter for use in evaluation and validation of high throughput screening assays
26. Miniaturization of a mammalian cell-based assay: Luciferase reporter gene readout in a 3 microliter 1536-well plate
27. Fluorescence correlation microscopy (FCM)-fluorescence correlation spectroscopy (FCS) taken into the cell