Towards a minimal motif for artificial transcriptional activators

Chemistry and Biology

Volumn 8, Issue 6, 2001, Pages 583-592

  Ansari, Aseem Z ^a   Mapp, Anna K ^b   Nguyen, Doan H ^b   Dervan, Peter B ^b   Ptashne, Mark ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b California Institute of Technology   (United States)

Author keywords

Chemical regulator; Hairpin polyamide; Small molecule activator; Targeted gene control

Indexed keywords

AMINO ACID SEQUENCE; AMINO ACID; CELL MEMBRANE; DNA BINDING; GENE ACTIVATION; GENE FUNCTION; OLIGOMERIZATION; POLYAMIDE; TRANSACTIVATION; TRANSCRIPTION REGULATION;

BASE SEQUENCE; BINDING SITES; DNA; HERPES SIMPLEX VIRUS PROTEIN VMW65; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

EID: 0034976453     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(01)00037-0     Document Type: Article

References (37)

1. Activation of gene expression by small molecule transcription factors
2. Transcriptional activation by recruitment
3. An activator target in the RNA polymerase II holoenzyme
4. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II
5. An RNA polymerase II holoenzyme responsive to activators
6. Transcription of eukaryotic protein-coding genes
7. Transcriptional activators direct histone acetyltransferase complexes to nucleosomes
8. Promoter targeting and chromatin remodeling by the SWI/SNF complex
9. Dimeric ligands define a role for transcriptional activation domains in reinitiation
10. A nonnatural transcriptional coactivator
11. A general strategy to enhance the potency of chimeric transcriptional activators
12. Gene activation by triplex-forming oligonucleotide coupled to the activating domain of protein VP16
13. Positive and negative regulation of endogenous genes by designed transcription factors
14. Sequence-specific DNA recognition by polyamides
15. On the pairing rules for recognition in the minor groove of DNA by pyrrole-imidazole polyamides
16. Recognition of the four Watson-Crick base pairs in the DNA minor groove by synthetic ligands
17. Regulation of gene expression by small molecules
18. Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands
19. Specific gain- and loss-of-function phenotypes induced by satellite-specific DNA-binding drugs fed to Drosophila melanogaster
20. GAL4-VP16 is an unusually potent transcriptional activator
21. Quantitation of putative activator-target affinities predicts transcriptional activating potentials
22. The transcriptional activator GCN4 contains multiple activation domains that are critically dependent on hydrophobic amino acids
23. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions
24. A minimal transcription activation domain consisting of a specific array of aspartic acid and leucine residues
25. Modulation of promoter occupancy by cooperative DNA/binding and activation-domain function is a major determinant of transcriptional regulation by activators in vivo
26. Modulating the potency of an activator in a yeast in vitro transcription system
27. Transcriptional activation modulated by homopolymeric glutamine and proline stretches
28. Mutational analysis of the transcriptional activation domain of RelA: Identification of a highly synergistic minimal acidic activation domain
29. Transcription in yeast activated by a putative amphipathic α helix linked to a DNA binding unit
30. A structural basis for recognition of A·T and T·A base pairs in the minor groove of B-DNA
31. Structural basis for G·C recognition in the DNA minor groove
32. Solid phase synthesis of polyamides containing imidazole and pyrrole amino acids
33. Preparation of thioesters for the ligation of peptides with non-native substrates
34. Quantitative DNase footprint titration - A method for studying protein-DNA interactions
35. Inhibition of major-groove-binding proteins by pyrrole-imidazole polyamides with an Arg-Pro-Arg positive patch
36. A transcriptional activating region with two contrasting modes of protein interaction