Combined use of ESI-MS and UV diode-array detection for localization of disulfide bonds in proteins: Application to an α-L-fucosidase of pea

Journal of Peptide Research

Volumn 57, Issue 6, 2001, Pages 473-482

  Codina, A ^a,b   Fernandez I ^a,b   Giralt, E ^a,b   Martinez I ^a,b,c   Ludevid, D ^a,b  

^a UNIVERSITY OF BARCELONA   (Spain)

^b DEPT OF ENVIRONMENTAL CHEMISTRY   (Spain)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

L fucosidase; 4 vinylpyridine; Diode array; Disulfide bonds; ESI MS; Tryptic digestion

Indexed keywords

ALPHA LEVO FUCOSIDASE;

ARTICLE; DISULFIDE BOND; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PEA; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN LOCALIZATION; ULTRAVIOLET IRRADIATION;

ALGORITHMS; ALPHA-L-FUCOSIDASE; AMINO ACID SEQUENCE; AUTOMATIC DATA PROCESSING; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DISULFIDES; MOLECULAR SEQUENCE DATA; PEAS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROPHOTOMETRY, ULTRAVIOLET; TRYPSIN;

PISUM SATIVUM;

EID: 0034974474     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2001.00859.x     Document Type: Article

References (20)

1. Disulfide bond assignment of ω-agatoxins IVB and IVC: Discovery of D-serine residue in ω-agatoxin IVB
2. Disulphide bonds and protein stability
3. Disulfide characterization of CD31 (PECAM)
4. Intrachain disulfide bond in the core hinge region of human IgG4
5. Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic α-amylase MaIS of Escherichia coli
6. A novel methodology for assignment of disulfide bond pairing in proteins
7. Modification of cysteine residues by alkylation. A tool peptide mapping protein identification
8. The disulfide bond structure of Plasmodium apical membrane antigen-1
9. Purification, characterization and cell wall localization of an α-fucosidase that inactivates a xyloglucan oligosaccharin
10. Molecular cloning and pattern of expression of an α-L-fucosidase gene from pea seedlings
11. S-Pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: A rapid mass spectrometric method for identifying cysteine-containing peptides
12. Amino acid sequences of Kunitz family subtilisin inhibitors from seeds of Canavalia lineata
13. Mammalian α-L-fucosidases
14. A developmentally regulated gene product from Dictyostelium discoideum shows high homology to human α-L-fucosidase
15. Crystallization and preliminary diffraction studies of Erythrina trypsin inhibitor
16. Crystallization and preliminary X-ray studies of psophocarpin B1, a chymotrypsin inhibitor from winged bean seeds
17. Purification and characterization of two Kunitz family subtilisin inhibitors from seeds of Canavalia lineata
18. Novel anti-platelet aggregation polypeptides from Vipera lebetina venom: Isolation and characterization
19. Reductive cleavage of cystine disulfides with tributylphosphine
20. Strategies for locating disulfide bonds in a monoclonal antibody via mass spectrometry