S-Pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: A rapid mass spectrometric method for identifying cysteine-containing peptides

Electrophoresis

Volumn 17, Issue 5, 1996, Pages 907-917

  Moritz, Robert L ^a   Eddes, James S ^a   Reid, Gavin E ^a   Simpson, Richard J ^a,b  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b ROYAL MELBOURNE HOSPITAL   (Australia)

Author keywords

Capillary liquid chromatogmphy; Mass spectrometry; Peptide mass fingerprinting; Two dimensional gel polyacrylamide gel electrophoresis

Indexed keywords

AMIDES; AMINO ACIDS; COVALENT BONDS; DRUG PRODUCTS; ELECTROPHORESIS; ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDES; SILICA; SULFUR COMPOUNDS;

ACRYLAMIDES; CAPILLARY LIQUID CHROMATOGMPHY; CYSTEINE-CONTAINING PEPTIDES; EDMAN DEGRADATION; PEPTIDE FRAGMENTS; PEPTIDE MASS FINGERPRINTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; TANDEM-MASS SPECTROMETRY; TWO-DIMENSIONAL GEL POLYACRYLAMIDE GEL ELECTROPHORESIS; TWO-DIMENSIONAL GELS;

MASS SPECTROMETRY;

CONFERENCE PAPER; DERIVATIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN DEGRADATION; TECHNIQUE;

EID: 0030043090     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150170512     Document Type: Conference Paper

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