Genetic and protein engineering of diagnostic enzymes, cholesterol oxidase and xylitol oxidase

Journal of Bioscience and Bioengineering

Volumn 91, Issue 5, 2001, Pages 433-441

  Murooka, Yoshikatsu ^a   Yamashita, Mitsuo ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Biotechnology; Cholesterol oxidase; Protein engineering; Streptomyces; Xylitol oxidase

Indexed keywords

CHOLESTEROL; ENZYMES; GENES;

SUBSTRATE-SPECIFIC ENZYMES;

BIOTECHNOLOGY;

CHOLESTEROL OXIDASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; XYLITOL OXIDASE;

BIOTECHNOLOGY; CATALYSIS; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENETIC ENGINEERING; LABORATORY DIAGNOSIS; NONHUMAN; REVIEW; STREPTOMYCES; THERMOSTABILITY;

EID: 0034972186     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80270-X     Document Type: Review

References (74)

1. Mammalian HMG-CoA reductase and its regulation
2. Microbial transformation of steroids. Part I: Decomposition of cholesterol by microorganisms
3. A method to screen anticholesterol substances produced by microbes and a new cholesterol oxidase produced by Streptomyces violascens
4. Microbial transformation of sterols. Part II: Cleavage of sterol side chains by microorganisms
5. 22 acid intermediates in the microbiological cleavage of the cholesterol side chains
6. The mechanism of microbial conversion of cholesterol into 17-ketosteroids
7. Studies on the microbiological degradation of cholesterol
8. Properties of crystalline 3β-hydroxysteroid oxidase of Brevibacterium sterolicam
9. 5-isomerase component of cholesterol oxidase
10. 5-3-ketosteroid isomerase reaction: Catalytic mechanism, specificity and inhibition
11. 5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and X-ray diffraction methods
12. Comparative studies on cholesterol oxidases from different sources
13. Purification of 3β-hydroxysteroid oxidase of Streptomyces violascens origin by affinity chromatography on cholesterol
14. Identification of the covalently bound flavine prosthetic group of cholesterol oxidase
15. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum
16. The kinetics of biosynthesis and some properties of an extracellular cholesterol oxidase produced by Arthrobacter sp. IM79
17. The kinetics of cholesterol oxidase synthesis by Nocardia rhodochrous
18. Microbiological degradation of sterols. II. Isolation of Rhodococcus sp. IM58 mutants with a block of the cholesterol degradation pathway
19. Identification of cholesterol oxidase from fast-growing mycobacterial strains and Rhodococcus sp.
20. Enzymatic determination of total serum cholesterol
21. Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702
22. Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp.
23. An operon containing the genes for cholesterol oxidase and a cytochrome P450-like protein from Streptomyces sp.
24. Cloning and expression of an extracellular agarase from Streptomyces coelicolor A3(2) in Streptomyces lividans 66
25. Heterologous expression of the α-amylase inhibitor gene cloned from an amplified sequence of Streptomyces tendae
26. Molecular cloning and expression in Streptomyces lividans of a proteinous alpha-amylase inhibitor (Haim II) gene from Streptomyces griseosporusi
27. Synthesis of bovine growth hormone by Streptomyces lividans
28. Cloning and expression of human interleukin 2 in Streptomyces lividans and the Escherichia coli consensus promoter
29. Secretory synthesis of human interleukin-2 by Streptomyces lividans
30. Secretory overproduction of Streptomyces cholesterol oxidase by Streptomyces lividans with a multi-copy shuttle vector
31. Isolation and identification of gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis
32. Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterlicum: Comparison with choA of Streptomyces sp. SA-COO
33. Genetic modification of the Streptomyces cholesterol oxidase gene for expression in Escherichia coli and development of promoter-probe vectors for use in enteric bacteria
34. Bacterial cholesterol oxidases are able to act as flavoprotein-linked ketosteroid mono-oxygenases that catalyse the hydroxylation of cholesterol to 4-cholesten-6-ol-3-one
35. Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 Å resolution
36. Crystal structure of cholesterol oxidase complexed with a steroid substrate: Implications for flavin adenine dinucleotide dependent alcohol oxidases
37. Improvement of thermal stability of a diagnostic enzyme, Streptomyces cholesterol oxidase, by random and site-directed mutageneses and a structural interpretation
38. Modelling and structurol-function relationships of cholesterol oxidase
39. Improvement of thermal stability of Streptomyces cholesterol oxidase by random mutagenesis and a structural interpretation
40. Development of improved cholesterol oxidase and its application
41. Effect of non-ionic detergents on apparent enzyme mechanism: V121A mutant of Streptomyces cholesterol oxidase endowed with enhanced sensitivity towards detergents
42. Separation of the two reactions, oxidation and isomerization, catalyzed by Streptomyces cholesterol oxidase
43. Isomerization, but not oxidation, is suppressed by a single point mutation, E361Q, in the reaction catalyzed by cholesterol oxidase
44. The isomerization catalyzed by Brevibacterium sterolicum cholesterol oxidase proceeds stereospecifically with one base
45. Cholesterol oxidase: A potential insecticidal protein active against boll weevil larvae
46. Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts
47. Introduction and expression of the Streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
48. Development of a host-vector system for Lactobacillus plantarum L137 isolated from a traditional fermented food produced in the Philippines
49. Characterization of pRGO1, a plasmid from Propionibacterium acidipropionici, and its use for development of a host-vector system in propionibacteria
50. Expression of Streptomyces sp. cholesterol oxidase in Lactobacillus casei
51. Native promoter-plasmid vector for heterologous cholesterol oxidase synthesis in Streptococcus thermophilus
52. Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coil
53. Molecular characterization of Lactobacills plantarum genes for β-ketoacyl-acyl carrier protein synthase III (fabH) and acetyl coenzyme A carboxylase (accBCDA) that are essential for fatty acid biosynthesis
54. Construction of an expression vector for propionibacteria and its use for production of δ-aminolevulinic acid (ALA) in Propionibacterium freudenreichii
55. Xylitol chewing gum and dental caries
56. Factors that affect the biosynthesis of xylitol by xylose-fermenting yeasts
57. D-xylulose-induced depletion of ATP and Pi in isolated rat hepatocytes
58. Effect of excess xylitol on nitrogen and glucose metabolism in parenterally fed rats
59. Xylitol
60. Determination of blood xylitol level in clinical tests
61. Determination of xylitol on human urine by gas-liquid chromatography
62. Determination of xylitol in the human serum and saliva by ion chromatography with pulsed amperometric detection
63. Enzymatische bestimmung von xylit in biologische
64. Xylitol absorption in healthy man
65. Purification and properties of D-xylulose reductase from Pachysolen tannophilus
66. Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae
67. Isolation and characterization of two xylitol dehydrogenases from Aspergillus niger
68. Purification and characterization of xylitol dehydrogenase from Neurospora crassa
69. Isolation and identification of xylitol dehydrogenase gene from Trichoderma reesei
70. Isolation, characterization, and molecular cloning of a thermostable xylitol oxidase from Streptomyces sp. IKD472
71. Isolation and some properties of sorbitol oxidase from Streptomyces sp. H-7775
72. Molecular cloning and expression of a gene encoding a novel sorbitol oxidase from Streptomyces sp. H-7775