Improvement of thermal stability of Streptomyces cholesterol oxidase by random mutagenesis and a structural interpretation

Protein Engineering

Volumn 10, Issue 3, 1997, Pages 231-235

  Nishiya, Yoshiaki ^a   Harada, Naoko ^a   Teshima, Shin Ichi ^a   Yamashita, Mitsuo ^b   Fujii, Isao ^c   Hirayama, Noriaki ^c   Murooka, Yoshikatsu ^b  

^a TOYOBO CO LTD   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c TOKAI UNIVERSITY   (Japan)

Author keywords

Cholesterol oxidase; Random mutagenesis; Streptomyces; Structural characterization; Thermostable mutants

Indexed keywords

AMINO ACID; CHOLESTEROL OXIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME STABILITY; ENZYME STRUCTURE; MUTAGENESIS; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STREPTOMYCES; THERMOSTABILITY;

STREPTOMYCES;

EID: 0030980084     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.3.231     Document Type: Article

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