One-step purification of a fully active hexahistidine-tagged human hexokinase type I overexpressed in Escherichia coli

Protein Expression and Purification

Volumn 22, Issue 1, 2001, Pages 38-44

  Palma, Francesco ^a   Longhi, Silvia ^a   Agostini, Deborah ^a   Stocchi, Vilberto ^a  

^a UNIVERSITY OF URBINO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME INHIBITOR; ENZYME MODIFICATION; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; GENE OVEREXPRESSION; GENE TAGGING; HEXOKINASE; HEXOSE; ISOENZYME; METAL CHELATE; PHOSPHATE; RECOMBINANT ENZYME;

ESCHERICHIA COLI; MAMMALIA;

EID: 0034969992     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1404     Document Type: Article

References (39)

1. Hexokinase
2. Hexokinases and glucokinases
3. Human hexokinase: Sequences of amino- and carboxyl-terminal halves are homologous
4. Complete amino acid sequence of rat brain hexokinase, deduced from the cloned cDNA, and proposed structure of a mammalian hexokinase
5. Glucose phosphorylation in tumor cells. Cloning, sequencing, and overexpression in active form of a full-length cDNA encoding a mitochondrial bindable form of hexokinase
6. Complete amino acid sequence of the type III isozyme of rat hexokinase, deduced from the cloned cDNA
7. Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: Comparison with a hexokinase from Novikoff ascites tumor
8. The amino acid sequence of rat liver glucokinase deduced from cloned cDNA
9. "Homology" in proteins and nucleic acids: A terminology muddle and a way out of it
10. Isolation and characterization of the discrete N- and C-terminal halves of rat brain hexokinase: Retention of full catalytic activity in the isolated C-terminal half
11. Structure/function relationships in hexokinase: Site-directed mutational analyses and characterization of overexpressed fragments implicate different functions for the N- and C-terminal halves of the enzyme
12. Functional organization of mammalian hexokinases: Characterization of the rat type III isozyme and its chimeric forms, constructed with the N- and C-terminal halves of the type I and type II isozymes
13. Functional organization of mammalian hexokinases: Both N- and C-terminal halves of the rat type II isozyme possess catalytic sites
14. Studies on the mechanism of orthophosphate regulation of bovine brain hexokinase
15. Pig red blood cell hexokinase: Evidence for the presence of hexokinase types II and III, and their purification and characterization
16. Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein
17. Structural determinants for the intracellular localization of the isozymes of mammalian hexokinase: Intracellular localization of fusion constructs incorporating structural elements from the hexokinase isozymes and the green fluorescent protein
18. An introduction to the isoenzymes of mammalian hexokinase types I-III
19. An intact hydrophobic N-terminal sequence is critical for binding of rat brain hexokinase to mitochondria
20. Characterization of hexokinase isoenzyme types I and II in ascites tumor cells by an interaction with mitochondrial membrane
21. Human hexokinase II mRNA and gene structure
22. Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization
23. Human pancreatic beta-cell glucokinase: cDNA sequence and localization of the polymorphic gene to chromosome 7, band p 13
24. Human hexokinase II: Localization of the polymorphic gene to chromosome 2
25. Energy metabolism of tumor cells: Requirement for a form of hexokinase with a propensity for mitochondrial binding
26. Intracellular localization and properties of particulate hexokinase in the Novikoff ascites tumor: Evidence for an outer mitochondrial membrane location
27. Functional significance of mitochondrial bound hexokinase in tumor cell metabolism: Evidence for preferential phosphorylation of glucose by intramitochondrially generated ATP
28. Expression of human brain hexokinase in Escherichia coli: Purification and characterization of the expressed enzyme
29. High-level expression and purification of a human "mini"-hexokinase
30. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
31. Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials: Serum protein affinities for gel-immobilized iron and nickel ions
32. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues
33. Expression and purification of hexahistidine-tagged human glutathione S-transferase P1-1 in Escherichia coli
34. Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3′-5′ exonuclease activity
35. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
36. Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins - Evaluation of two anti-His-tag monoclonal antibodies