Mass spectrometric study of the Escherichia coli repressor proteins, IclR and GclR, and their complexes with DNA

Protein Science

Volumn 10, Issue 7, 2001, Pages 1370-1380

  Donald, Lynda J ^a   Hosfield, David J ^a   Cuvelier, Susan L ^a   Werner, Ens ^a   Standing, Kenneth G ^a   Duckworth, Harry W ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

IclR family protein; Nanospray ionization; Noncovalent; Protein DNA interaction; Time of flight mass spectrometry

Indexed keywords

DNA; DOUBLE STRANDED DNA; LIGASE; POTASSIUM CHLORIDE; PROTEIN GCIR; PROTEIN ICIR; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; COVALENT BOND; DATA BASE; ESCHERICHIA COLI; GENE CONTROL; GENE SEQUENCE; IONIZATION; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DNA BINDING; PROTEIN STABILITY; PROTEIN SYNTHESIS; SEQUENCE HOMOLOGY; SOLUBILITY; STOICHIOMETRY;

BACTERIAL PROTEINS; CONSENSUS SEQUENCE; DNA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION; ISOCITRATE LYASE; MASS SPECTROMETRY; OPERON; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034967293     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.780101     Document Type: Article

References (62)

1. The Pfam protein families database
2. Nucleotide sequence of the putative regulatory gene and major promoter region of the Streptomyces griseus glycerol opeton
3. The role of acetate in isocitrate lyase induction
4. Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase
5. Orthogonal-injection time-of-flight mass spectrometry for analysis of biomolecules
6. Glyoxylate bypass operon of Escherichia coli: Cloning and determination of the functional map
7. Regulation of the acetate operon in Escherichia coli: Purification and functional characterization of the IclR repressor
8. Amber mutants of the α-ketoglutarate dehydrogenase gene of Escherichia coli K12
9. Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli
10. Assay methods for key enzymes of the glyoxylate cycle
11. Preparation and properties of pure, full-length IclR protein of Escherichia coli. Use of time-of-flight mass spectrometry to investigate the problems encountered
12. ESI/TOF measurements of a noncovalent complex between lactose repressor protein (LacI) and double-stranded DNA containing its specific operator sequence
13. Characterization of the IclR protein and its complex with two different ds DNA fragments
14. Spectroscopic determination of tryptophan and tyrosine in proteins
15. The putative regulator of catechol catabolism in Rhodococcus opacus 1CP - An IclR-type, not a LysR-type transcriptional regulator
16. Characterization of a protocatechuate catabolic gene cluster from Rhodococcus opacus ICP: Evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity
17. Genetic characterization and expression in heterologous hosts of the 3-3-(hydroxyphenyl)-propionate catabolic pathway of Escherichia coli K-12
18. PcaU, a transcriptional activator of genes for protocatechuate utilization in Acinetobacter
19. Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon
20. PcaR-mediated activation and repression of pca genes from Pseudomonas putida are propagated by its binding to both the -35 and the -10 promoter elements
21. Glyoxylate carboligase of Escherichia coli: A flavoprotein
22. Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): A vehicle for direct determination of three-dimensional structure
23. Biochemical and genetic studies with lysine + methionine mutants of Escherichia coli: Lipoic acid and α-ketoglutarate dehydrogenase-less mutants
24. Studies with α-ketoglutarate dehydrogenase mutants of Escherichia coli
25. Mutation analysis of PobR and PcaU, closely related transcriptional activators in Acinetobacter
26. The role of acetate in isocitrate lyase induction
27. The role and control of the glyoxylate cycle
28. Lac repressor forms loops with linear DNA carrying the suitably spaced lac operators
29. A collisional damping interface for an electrospray ionization time-of-flight mass spectrometer
30. Studies of noncovalent complexes in an electrospray ionization/time-of-flight mass spectrometer
31. A single gene codes for the kinase and phosphatase which regulate isocitrate dehydrogenase
32. Tandem binding in crystals of a trp repressor/operator half-site complex
33. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
34. Ecc, and a global RNA regulator in Erwinia carotovora subsp. carotovora
35. Protein measurement with the Folin phenol reagent
36. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12
37. Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2
38. Specific interactions between the IclR repressor of the acetate operon of Escherichia coli and its operator
39. The pir gene of Erwinia chrysanthemi EC16 regulates hyperinduction of pectate lyase virulence genes in response to plant signals
40. Self-regulation of pir, a regulatory protein responsible for hyperinduction of pectate lyase in Erwinia chrysanthemi EC16
41. 'Waterbug' dialysis
42. Crystal structure of trp repressor/operator complex at atomic resolution
43. A reactive, surface cysteine residue of the class-II fructose-1,6-biphosphate aldolase of Escherichia coli revealed by electrospray ionisation mass spectrometry
44. The binding site of the IclR repressor protein overlaps the promoter of aceBAK
45. Study of a noncovalent trp repressor: DNA operator complex by electrospray ionization time-offlight mass spectrometry
46. The interactions of Escherichia coli trp repressor with tryptophan and with an operator nucleotide
47. DNA sequencing with chain-terminating inhibitors
48. Rapid 'de novo' peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer
49. Cloning and transcription analysis of the entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) and identification of a closely associated transcription unit
50. Structure and regulation of controlling sequences for the Streptomyces coelicolor glycerol operon
51. Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): Sequence analysis and biochemical properties of a third family of extradiol dioxygenases
52. Purification, characterization and primary structure of Escherichia coli protease VII with specificity for paired basic residues: Identity of protease VII with ompT
53. Regulation of the glyoxylate bypass operon: Cloning and characterization of IclR
54. Target detection assay (TDA): A versatile procedure to determine DNA binding sites as demonstrated on SPl protein
55. Erwinia carotovora has two KdgR-like proteins belonging to the IclR family of transcriptional regulators: Identification and characterization of the RexZ activator and the KdgR repressor of pathogenesis
56. Physiologie et génétique de l'isocitratase et des malate synthases chez Escherichia coli K12
57. Reflecting time-offlight mass spectrometer with an electrospray ion source and orthogonal extraction
58. Analytical properties of the nanoelectrospray ion source
59. The solution structures of the trp repressor-operator complex