PcaR-mediated activation and repression of pca genes from Pseudomonas putida are propagated by its binding to both the -35 and the -10 promoter elements

Molecular Microbiology

Volumn 32, Issue 2, 1999, Pages 253-263

  Guo, Zhu ^a   Houghton, John E ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADIPIC ACID; BINDING PROTEIN; DIMER; PROTOCATECHUIC ACID; REGULATOR PROTEIN;

ARTICLE; BACTERIAL GENE; CATABOLISM; CONTROLLED STUDY; CORRELATION FUNCTION; DIMERIZATION; DNA FOOTPRINTING; DNA SEQUENCE; GENE ACTIVATION; GENE OVEREXPRESSION; GENE REPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN FAMILY; PROTEIN PURIFICATION; PSEUDOMONAS PUTIDA; SEQUENCE HOMOLOGY;

ADIPIC ACIDS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; DEOXYRIBONUCLEASE I; DNA FOOTPRINTING; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HYDROXYBENZOIC ACIDS; MOLECULAR SEQUENCE DATA; OPERATOR REGIONS (GENETICS); PROMOTER REGIONS (GENETICS); PSEUDOMONAS PUTIDA; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

EID: 0032923396     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01342.x     Document Type: Article

References (40)

1. Ansari, A.Z., Bradner, J.E., and O'Halloran, T.V. (1995) DNA-bend modulation in a repressor-to-activator switching mechanism. Nature 374: 371-374.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., et al. (1992-98) Current Protocols in Molecular Biology. New York: J. Wiley & Sons.
3. Caslake, L.F., Ashraf, S.I., and Summers, A.O. (1997) Mutations in the alpha and sigma-70 subunits of RNA polymerase affect expression of the mer operon. J Bacteriol 179: 1787-1795.
4. Choy, H.E., Park, S.W., Aki, T., Parrack, P., Fujita, N., Ishihama, A., et al. (1995) Repression and activation of transcription by Gal and Lac repressors: involvement of alpha subunit of RNA polymerase. EMBO J 14: 4523-4529.
5. Dombroski, A.J., Johnson, B.D., Lonetto, M., and Gross, C.A. (1996) The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing. Proc Natl Acad Sci USA 93: 8858-8862.
6. Farinha, M.A., and Kropinski, A.M. (1990) Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters. J Bacteriol 172: 3496-3499.
7. Fried, M., and Crothers, D.M. (1983) Equilibria and kinetics of lac repressor - operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res 23: 6505-6525.
8. Gao, J., and Gussin, G.N. (1991) RNA polymerase from Pseudomonas aeruginosa and Pseudomonas syringae respond to Escherichia coli activator proteins. J Bacteriol 173: 394-397.
9. Guo, Z., and Houghton, J.E. (1998) Purification and characterization of the transcriptional regulator PcaR from Pseudomonas putida. In Proceedings of the American Society for Microbiology Meetings, Atlanta, GA. H25.
10. Harwood, C.S., and Parales, R.E. (1996) The β-ketoadipate pathway and the biology of self-identity. Annu Rev Microbiol 50: 553-590.
11. deHaseth, P.L., Zupanic, M., and Record, M. (1998) RNA polymerase promoter interactions: the comings and goings of RNA polymerase. J Bacteriol 180: 3019-3025.
12. Hendrickson, W., and Schleif, R.B. (1984) Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay. J Mol Biol 178: 611-628.
13. Hidalgo, E., and Demple, B. (1994) An iron-sulfur center is essential for transcriptional activation by the redox-sensing SoxR protein. EMBO J 13: 138-146.
14. Hidalgo, E., and Demple, B. (1997) Spacing of promoter regulates the basal expression of the soxS gene and converts SoxR from a transcriptional activator into a repressor. EMBO J 16: 1056-1065.
15. Hochschild, A., and Dove, S.L. (1998) Protein-protein contacts that activate and repress prokaryotic transcription. Cell 92: 597-600.
16. Houghton, J.E., and Shanley, M.S. (1994) Catabolic potential of pseudomonads: a regulatory perspective. In Biological Degradation and Bioremediation of Toxic Chemicals. Chaudhry, R. (ed.). Portland, OR: Discorides Press, pp. 11-32.
17. Hughes, E.J., Shapiro, M.J., Houghton, J.E., and Ornston, L.N. (1988) Cloning and expression of pca genes from Pseudomonas putida in Escherichia coli. J Gen Microbiol 134: 2877-2887.
18. Lobell, R.B., and Schleif, R.F. (1990) DNA looping and unlooping by AraC protein. Science 250: 528-532.
19. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 269-275.
20. Lu, C.-D., Houghton, J.E., and Abdelai, A.T. (1992) Characterization of the arginine repressor from Salmonella typhimurium and its interactions with the carAB operator. J Mol Biol 225: 11-24.
21. McLean, B.W., Wiseman, S.L., and Kropinski, A.M. (1997) Functional analysis of sigma-70 consensus promoters in Pseudomonas aeruginosa and Escherichia coli. Can J Microbiol 43: 981-985.
22. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
23. Monsalve, M., Mencia, M., Rojo, F., and Salas, M. (1996) Activation and repression of transcription at two different phage f29 promoters are mediated by interaction of the same residues of regulatory protein p4 with RNA polymerase. EMBO J 15: 383-391.
24. Monsalve, M., Mencia, M., Salas, M., and Rojo, F. (1997) Transcription activation or repression by phage f29 protein p4 depends on the strength of the RNA polymerase-promoter interactions. Mol Cell 1: 99-107.
25. Mooney, R.A., Artsimovitch, I., and Landick, R. (1998) Information processing by RNA polymerase: recognition of regulatory signals during RNA chain elongation. J Bacteriol 180: 3265-3275.
26. Nègre, D., Oudot, C., Prost, J.-F., Murakam, K., Ishihami, A., Cozzone, A.J., et al. (1998) FruR-mediated activation at the ppsA promoter of Escherichia coli. J Mol Biol 276: 355-365.
27. Nichols, N.N., and Harwood, C.S. (1997) PcaK, a high affinity permease for the aromatic compounds 4-hydroxybenzoate and protocatechuate from Pseudomonas putida. J Bacteriol 179: 5056-5061.
28. Ornston, L.N. (1966) The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. II. Enzymes of the protocatechuate pathway. J Biol Chem 241: 3787-3794.
29. Parales, R.E., and Harwood, C.S. (1992) Characterization of the genes encoding β-ketoadipate; succinyl-coenzyme A transferase in Pseudomonas putida. J Bacteriol 174: 4657-4666.
30. Parales, R.E., and Harwood, C.H. (1993) Regulation of the pcal, J genes for aromatic degradation in Pseudomonas putida. J Bacteriol 175: 5829-5838.
31. Park, S.-J., Wireman, J., and Summers, A.O. (1992) Genetic analysis of the Tn21 mer promoter-operator. J Bacteriol 174: 2160-2171.
32. Parke, D., and Ornston, L.N. (1976) Constitutive synthesis of the enzymes of the protocatechuate and of the β-ketoadipate uptake system in mutant strains of Pseudomonas putida. J Bacteriol 126: 271-281.
33. Ptashne, M. (1992) Phage I and Higher Organisms. Oxford: Cell Press and Blackwell Scientific Publications.
34. Romero-Steiner, S., Parales, R., Harwood, C.S., and Houghton, J.E. (1994) Characterization of the pcaR regulatory gene for Pseudomonas putida, which is required for the complete degradation of p-hydrobenzoate. J Bacteriol 176: 5771-5779.
35. Schell (1993) Molecular biology of the LysR family of transcriptional regulators. Annu Rev Microbiol 47: 597-626.
36. Schlax, P.J., Capp, M.W., and Record, M.T.J. (1995) Inhibition of transcription initiation by lac repressor. J Mol Biol 245: 331-350.
37. Stanier, R.Y., Palleroni, N.J., and Douderoff, M. (1966) The aerobic Pseudomonads: a taxonomic study. J Gen Microbiol 48: 159-271.
38. Summers, A.O. (1992) Untwist and shout: a heavy metal-responsive transcriptional regulator. J Bacteriol 174: 3097-3101.
39. Tabor, S., and Richardson, C.A. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078.
40. Williams, D.R. (1993) Multifunctional repressor KorB can block transcription by preventing isomerization of RNA polymerase-promoter complexes. Nucleic Acids Res 21:1141-1148.