kdgR(Ecc) Negatively regulates genes for pectinases, cellulase, protease, Harpin(Ecc), and a global RNA regulator in Erwinia carotovora subsp. carotovora

Journal of Bacteriology

Volumn 181, Issue 8, 1999, Pages 2411-2421

  Liu, Yang ^a   Jiang, Guoqiao ^a   Cui, Yaya ^a   Mukherjee, Asita ^a   Ma, Wei Lei ^a   Chatterjee, Arun K ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; POLYGALACTURONASE; PROTEINASE; BACTERIAL PROTEIN; HARPIN; HRPZ PROTEIN, PSEUDOMONAS SYRINGAE; KDGR PROTEIN, ERWINIA CHRYSANTHEMI; OUTER MEMBRANE PROTEIN; REPRESSOR PROTEIN; RSMB PROTEIN, ERWINIA CAROTOVORA; TRANSCRIPTION FACTOR;

AGRICULTURE; ARTICLE; BACTERIAL VIRULENCE; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; NONHUMAN; PECTOBACTERIUM CAROTOVORUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPRESSOR GENE; AMINO ACID SEQUENCE; BINDING SITE; BIOLOGICAL MODEL; BIOSYNTHESIS; COMPARATIVE STUDY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLANT DISEASE; PROTEIN BINDING; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CAROTOVORA; ERWINIA CHRYSANTHEMI; ESCHERICHIA COLI; NEGIBACTERIA; PECTOBACTERIUM CAROTOVORUM; PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM; PROKARYOTA;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CELLULASE; CLONING, MOLECULAR; ENDOPEPTIDASES; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PECTOBACTERIUM CAROTOVORUM; PLANT DISEASES; POLYGALACTURONASE; PROTEIN BINDING; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION FACTORS;

EID: 0032587947     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.8.2411-2421.1999     Document Type: Article

References (57)

1. Aiba, H., S. Adhya, and B. de Crombrugghe. 1981. Evidence for two functional gal promoters in intact Escherichia coli cells. J. Biol. Chem. 256:11905-11910.
2. Alfano, J. R., and A. Collmer. 1996. Bacterial pathogens in plants: life up against the wall. Plant Cell 8:1683-1698.
3. Barras, F., F. van Gijsegem, and A. K. Chatterjee. 1994. Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu. Rev. Phytopathol. 32: 201-234.
4. Ech: an elicitor of the hypersensitive response that contributes to soft-rot pathogenesis. Mol. Plant-Microbe Interact. 8:484-491.
5. Chatterjee, A., Y. Cui, Y. Liu, C. K. Dumenyo, and A. K. Chatterjee. 1995. Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone. Appl. Environ. Microbiol. 61:1959-1967.
6. Chatterjee, A., Y. Liu, and Arun K. Chatterjee. 1995. Nucleotide sequence of a pectate lyase structural gene, pell of Erwinia carotovora subsp. carotovora strain 71 and structural relationship of pell with other pel genes of Erwinia species. Mol. Plant-Microbe Interact. 8:92-95.
7. Chatterjee, A. K., G. E. Buchanan, M. K. Behrens, and M. P. Starr. 1979. Synthesis and excretion of polygalacturonic acid trans-eliminase in Erwinia, Yersinia, and Klebsiella species. Can. J. Microbiol. 25:94-102.
8. Chatterjee, A. K., J. L. McEvoy, H. Murata, and A. Collmer. 1991. Regulation of the production of pectinases and other extracellular enzymes in the soft-rotting Erwinia spp., p. 45-58. In S. S. Patil, S. Ouchi, D. Mills, and C. Vance (ed.), Molecular strategies of pathogens and host plants. Springer-Verlag, New York, N.Y.
9. Chatterjee, A. K., L. M. Ross, J. L. McEvoy, and K. K. Thurn. 1985. pULB113, an RP4::mini-Mu plasmid, mediates chromosomal mobilization and R-prime formation in Erwinia amylovora, Erwinia chrysanthemi, and subspecies of Erwinia carotovora. Appl. Environ. Microbiol. 50:1-9.
10. Collmer, A., and N. T. Keen. 1986. The role of pectic enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24:383-409.
11. Cooper, C. J. C., and G. P. C. Salmond. 1993. Molecular analysis of the major cellulase (CeIV) of Erwinia carotovora: evidence for an evolutionary "mix-and-match" of enzyme domains. Mol. Gen. Genet. 241:341-350.
12. Cui, Y., A. Chatterjee, Y. Liu, C. K. Dumenyo, and A. K. Chatterjee. 1995. Identification of a global repressor gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp. J. Bacteriol. 177:5108-5115.
13. Ecc and elicit a hypersensitive reaction-like response in tobacco leaves. Mol. Plant-Microbe Interact. 9:565-573.
14. Eriksson, R. B., A. R. Andersson, M. Pirhonen, and E. T. Palva. 1998. Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 11:743-752.
15. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of a plasmid RK2 depend on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
16. Frederick, R. D., J. Chiu, J. L. Bennetzen, and A. K. Handa. 1997. Identification of a pathogenicity locus, rpfA, in Erwinia carotovora subsp. carotovora that encodes a two-component sensor-regulator protein. Mol. Plant-Microbe Interact. 10:407-415.
17. Harris, S. J., Y.-L. Shih, S. D. Bentley, and G. P. C. Salmond. 1998. The hexA gene of Erwinia carotovora encodes a LysR homologue and regulates motility and the expression of multiple virulence determinants. Mol. Microbiol. 28: 705-717.
18. He, B., and H. Zalkin. 1992. Repression of Escherichia coli purB is by a transcriptional roadblock mechanism. J. Bacteriol. 174:7121-7127.
19. Hinton, J. C. D., and G. P. C. Salmond. 1987. Use of TnphoA to enrich for extracellular enzyme mutants of Erwinia carotovora subsp. carotovora. Mol. Microbiol. 1:381-386.
20. Hinton, J. C. D., J. M. Sidebotham, D. R. Gill, and G. P. C. Salmond. 1989. Extracellular and periplasmic isoenzymes of pectate lyase from Erwinia carotovora subspecies carotovora belong to different gene families. Mol. Microbiol. 3:1785-1795.
21. Hugouvieux-Cotte-Pattat, N., G. Condemine, W. Nasser, and S. Reverchon. 1996. Regulation of pectinolysis in Erwinia chrysanthemi. Annu. Rev. Microbiol. 50:213-257.
22. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70:191-197.
23. Kleiner, D., W. Paul, and M. J. Merrick. 1988. Construction of multicopy expression vectors for regulated overproduction of proteins in Klebsiella pneumoniae and other enteric bacteria. J. Gen. Microbiol. 134:1779-1784.
24. Jones, S., B. Yu, N. J. Bainton, M. Birdsall, B. W. Bycroft, S. R. Chhabra, A. J. R. Cox, P. Golby, P. J. Reeves, S. Stephens, M. K. Winson, G. P. C. Salmond, G. S. A. B. Stewart, and P. Williams. 1993. The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J. 12:2477-2482.
25. Liu, M. Y., G. Gui, B. Wei, J. F. Preston III, L. Oakford, Ü. Yüksel, D. P. Giedroc, and T. Romeo. 1997. The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli. J. Biol. Chem. 272:17502-17510.
26. Liu, Y., A. Chatterjee, and A. K. Chatterjee. 1994. Nucleotide sequence, organization and expression of rdgA and rdgB genes that regulate pectin lyase production in the plant pathogenic bacterium Erwinia carotovora subsp. carotovora in response to DNA damaging agents. Mol. Microbiol. 14:999-1010.
27. Liu, Y., A. Chatterjee, and A. K. Chatterjee. 1994. Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71. Appl. Environ. Microbiol. 60:2545-2552.
28. Liu, Y., Y. Cui, A. Mukherjee, and A. K. Chatterjee. 1998. Characterization of a novel RNA regulator of Erwinia carotovora subsp. carotovora that controls production of extracellular enzymes and secondary metabolites. Mol. Microbiol. 29:219-234.
29. Liu, Y., Y. Cui, A. Mukherjee, and A. K. Chatterjee. 1997. Activation of the Erwinia carotovora subsp. carotovora pectin lyase structural gene pnlA: a role for RdgB. Microbiology 143:705-712.
30. Liu, Y., X. Wang, A. Mukherjee, and A. K. Chatterjee. 1996. RecA relieves negative autoregulation of rdgA, which specifies a component of the RecA-Rdg regulatory circuit controlling pectin lyase production in Erwinia carotovora subsp. carotovora. Mol. Microbiol. 22:909-918.
31. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
32. Ecc gene, which encodes an elicitor of the hypersensitive reaction. Mol. Plant-Microbe Interact. 10:462-471.
33. Mukherjee, A., Y. Cui, Y. Liu, and A. K. Chatterjee. Unpublished data.
34. Mukherjee, A., Y. Cui, W. Ma, Y. Liu, A. Ishihama, A. Eisenstark, and A. K. Chatterjee. 1998. RpoS (sigma-S) controls the expression of rsmA, a global regulator of secondary metabolites, Hairpin, and extracellular proteins in Erwinia carotovora. J. Bacteriol. 180:3629-3634.
35. Murata, H., A. Chatterjee, Y. Liu, and A. K. Chatterjee. 1994. Regulation of the production of extracellular pectinase, cellulase, and protease in the soft rot bacterium Erwinia carotovora subsp. carotovora: evidence that aepH of Erwinia carotovora subsp. carotovora 71 activates gene expression in Erwinia carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli. Appl. Environ. Microbiol. 60:3150-3159.
36. Murata, H., J. L. McEvoy, A. Chatterjee, A. Collmer, and A. K. Chatterjee. 1991. Molecular cloning of an aepA gene that activates production of extracellular pectolytic, cellulolytic, and proteolytic enzymes in Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 4:239-246.
37. Nasser, W., J. Robert-Baudouy, and S. Reverchon. 1997. Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes. Mol. Microbiol. 26:1071-1082.
38. Nasser, W., S. Reverchon, G. Condemine, and J. Robert-Baudouy. 1994. Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis. J. Mol. Biol. 236:427-440.
39. Nasser, W., S. Reverchon, and J. Robert-Baudouy. 1992. Purification and functional characterization of the KdgR protein, a major repressor of pectinolysis genes of Erwinia chrysanthemi. Mol. Microbiol. 6:257-265.
40. Negre, D., J. C. Cortay, I. A. Old, A. Galinier, C. Richaud, I. Saint-Girons, and A. J. Cozzone. 1991. Overproduction and characterization of the iclR gene product of Escherichia coli K12 and comparison with that of Salmonella typhimurium LT2. Gene 97:29-37.
41. Pan, B., I. Unnikrishnan, and D. C. Laporte. 1996. The binding site of the IcIR repressor protein overlaps the promoter of aceBAK. J. Bacteriol. 178: 3982-3984.
42. Pérombélon, M. C. M., and A. Kelman. 1980. Ecology of the soft rot Erwinias. Annu. Rev. Phytopathol. 18:361-387.
43. Pirhonen, M., H. Saarilahti, M.-B. Karlsson, and E. T. Palva. 1991. Identification of pathogenicity determinants of Erwinia carotovora subsp. carotovora by transposon mutagenesis. Mol. Plant-Microbe Interact. 4:276-283.
44. Pirhonen, M., D. Flego, R. Heikinheimo, and E. T. Palva. 1993. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J. 12:2467-2476.
45. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
46. Reverchon, S., Y. Huang, C. Bourson, and J. Robert-Baudouy. 1989. Nucleotide sequences of the Erwinia chrysanthemi ogl and pelE genes, negatively regulated by the kdgR gene product. Gene 85:125-134.
47. Reverchon, S., W. Nasser, and J. Robert-Baudouy. 1991. Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation. Mol. Microbiol. 5:2203-2216.
48. Romeo, T. M. 1998. Global regulation by a small RNA-binding protein CsrA and the noncoding RNA molecule CsrB. Mol. Microbiol. 29:1321-1330.
49. Romeo, T., M. Gong, M. Y. Liu, and A. M. Brun-Zinkernagel. 1993 Identification and molecular characterization of csrA, a pleiotropic gene from Escherichia coli that affects glycogen biosynthesis, gluconeogenesis, cell size, and surface properties. J. Bacteriol. 175:4744-4755.
50. Shih, Y. L., S. Harris, S. Bentley, and G. P. C. Salmond. 1998. Coordinate regulation of exoenzymes and motility in Erwinia carotovora, abstr. 1.8.52. In 7th International Congress of Plant Pathology, Edinburgh, Scotland.
51. Smith, C. P., and K. F. Chater. 1988. Structure and regulation of controlling sequences for the Streptomyces coelicolor glycerol operon. J. Mol. Biol. 204: 569-580.
52. Spaink, H. P., R. J. H. Okker, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg. 1987. Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol. Biol. 9:27-39.
53. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
54. Thomson, N. R., A. Cox, B. W. Bycroft, G. S. A. B. Stewart, P. Williams, and G. P. C. Salmond. 1997. The Rap and Hor proteins of Erwinia, Serratia and Yersinia: a novel subgroup in a growing superfamily of proteins regulating diverse physiological processes in bacterial pathogens. Mol. Microbiol. 26: 531-544.
55. Uptain, S. M., C. M. Kane, and M. J. Chamberlin. 1997. Basic mechanisms of transcript elongation and its regulation. Annu. Rev. Biochem. 66:117-172.
56. Wharam, S. D., V. Mulholland, and G. P. C. Salmond. 1995. Conserved virulence factor regulation and secretion systems in bacterial pathogens of plants and animals. Eur. J. Plant Pathol. 101:1-13.
57. Zink, R. T., R. J. Kemble, and A. K. Chatterjee. 1984. Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica. J. Bacteriol. 157:809-814.