Structural and functional changes in bovine pancreatic ribonuclease A by the replacement of Phe120 with other hydrophobic residues

Journal of Biochemistry

Volumn 129, Issue 6, 2001, Pages 917-922

  Chatani, E ^a   Tanimizu, N ^a   Ueno, H ^a   Hayashi, R ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Carboxymethylation rate; Hydrophobicity; Phenylalanine; RNase; electron

Indexed keywords

AMINO ACID; PANCREATIC RIBONUCLEASE; PHENYLALANINE DERIVATIVE;

AMINO ACID SUBSTITUTION; ARTICLE; CATTLE; ENTHALPY; ENTROPY; HYDROPHOBICITY; METHYLATION; NONHUMAN; THERMODYNAMICS; THERMOSTABILITY;

BOS TAURUS; BOVINAE;

EID: 0034961734     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002937     Document Type: Article

References (33)

1. Bovine pancreatic ribonuclease
2. Ribonuclease A
3. Pancreatic ribonucleases
4. His...Asp catalytic dyad of ribonuclease A: Conformational stability of the wild-type, D121N, D121A, and H119A enzymes
5. a values in the wild-type, D121N, and D121A enzymes
6. His...Asp catalytic dyad of ribonuclease A: Structure and function of the wild type, D121N, and D121A enzymes
7. Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A
8. Coulombic forces in protein-RNA interactions: Binding and cleavage by ribonuclease A and variants at Lys7, Arg10, and Lys66
9. Coulombic effects on remote subsites on the active site of ribonuclease A
10. Preparation of pancreatic ribonuclease 1-114 and 1-115 and their reactivation by mixture with synthetic COOH-terminal peptides
11. Structural determinants of enzymatic processivity
12. The structures of RNase A complexed with 3′-CMP and d(CpA): Active site conformation and conserved water molecules
13. Role of Phe120 in the activity and structure of bovine pancreatic ribonuclease A
14. The nature of the amino acid residues involved in the inactivation of ribonuclease by iodoacetate
15. Alkylation and identification of the histidine residues at the active site of ribonuclease
16. Properties and conformation of the histidine residues at the active site of ribonuclease
17. The reactivities of the histidine residues at the active site of ribonuclease toward halo acids of different structures
18. Mechanisms of carboxymethylation of bovine pancreatic nucleases by haloacetates and tosylglycolate
19. Relationships between conformation of ribonuclease and its reactivity toward iodoacetate
20. a shifts accompanying the inactivating Asp121-Asn substitution in a semisynthetic bovine pancreatic ribonuclease
21. Transition-state analysis of the facilitated alkylation of ribonuclease A by bromoacetate
22. The structural roles of amino acid residues near the carboxyl terminus of bovine pancreatic ribonuclease A
23. Further studies on the alkylation of the histidine residues at the active site of pancreatic ribonuclease
24. Reactivation of des(119-, 120-, or 121-124) ribonuclease A by mixture with synthetic COOH-terminal peptide of varying length
25. Engineering ribonuclease A: Production, purification and characterization of wild-type enzyme and mutant at Gln11
26. Folding and unfolding kinetics of the proline-to-alanine mutants of bovine pancreatic ribonuclease A
27. Measurement of protein using bicinchoninic acid
28. The subsites structure of bovine pancreatic ribonuclease A accounts for the abnormal kinetic behavior with cytidine 2′,3′-cyclic phosphate
29. Comparing the polarities of the amino acids: Side-chain distribution coefficients between the vapor phase, cyclohexane, 1-octanol, and neutral aqueous solution
30. Energetics of ribonuclease A catalysis. 1. pH, ionic strength, and solvent isotope dependence of the hyrolysis of cytidine cyclic 2′,3′-phosphate
31. Structural investigation of catalytically modified F120L and F120Y semisynthetie ribonucleases
32. The refined crystal structure of semisynthetic ribonuclease at 1.8 Å resolution