High-level expression of three members of the murine angiogenin family in Escherichia coli and purification of the recombinant proteins

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 307-317

  Holloway, Daniel E ^a   Hares, Michelle C ^a   Shapiro, Robert ^b   Subramanian, Vasanta ^a   Acharya, K Ravi ^a  

^a University of Bath   (United Kingdom)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOGENIN; CATION EXCHANGE; CHROMATOGRAPHY; CYTOPLASM; EXPRESSION VECTOR; HETEROLOGOUS EXPRESSION; PANCREATIC RIBONUCLEASE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RENATURATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TRANSFER RNA;

ESCHERICHIA COLI; MURINAE;

EID: 0034958310     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1434     Document Type: Article

References (63)

1. A putative angiogenin receptor in angiogenin-responsive human endothelial cells
2. Angiogenin promotes invasiveness of cultured endothelial cells by stimulation of cell-associated proteolytic activities
3. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells
4. Amino acid sequence of human tumor derived angiogenin
5. Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor
6. Evolution of vertebrate ribonucleases: Ribonuclease A superfamily
7. Ribonuclease A
8. Eosinophil-associated ribonucleases
9. The eosinophil ribonucleases
10. Seminal ribonuclease: The importance of diversity
11. Ribonuclease 4, an evolutionarily highly conserved member of the superfamily
12. Ribonuclease k6: Chromosomal mapping and divergent rates of evolution within the RNase A gene superfamily
13. Actin is a binding protein for angiogenin
14. Nuclear translocation of angiogenin in proliferating endothelial cells is essential to its angiogenic activity
15. Site-directed mutagenesis of histidine-13 and histidine-114 of human angiogenin. Alanine derivatives inhibit angiogenin-induced angiogenesis
16. Characteristic ribonucleolytic activity of human angiogenin
17. Expression of Met-(-1) angiogenin in Escherichia coli: Conversion to the authentic
18. A covalent angiogenin/ribonuclease hybrid with a fourth disulfide bond generated by regional mutagenesis
19. Role of glutamine-117 in the ribonucleolytic activity of human angiogenin
20. Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease
21. Refined crystal structures of native human angiogenin and two active site variants: Implications for the unique functional properties of an enzyme involved in neovascularisation during tumour growth
22. a values
23. Structural features that determine the enzymatic potency and specificity of human angiogenin: Threonine-80 and residues 58-70 and 116-123
24. A monoclonal antibody to human angiogenin suppresses tumor growth in athymic mice
25. Angiogenin antagonists prevent tumor growth in vivo
26. The widespread expression of angiogenin in different human cells suggests a biological function not only related to angiogenesis
27. Tissue-specific expression of pancreatic-type RNases and RNase inhibitor in humans
28. Isolation of angiogenin from normal human plasma
29. The complete amino acid sequence of bovine milk angiogenin
30. Isolation and sequencing of mouse angiogenin DNA
31. The mouse angiogenin gene family: Structures of an angiogenin-related protein gene and two pseudogenes
32. E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts
33. The I.M.A.G.E. consortium: An integrated molecular analysis of genomes and their expression
34. Evolution of the rodent eosinophil-associated RNase gene family by rapid gene sorting and positive selection
35. Localization of the human angiogenin gene to chromosome band 14q11, proximal to the T cell receptor alpha/delta locus
36. Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: Evidence for intronless coding sequences in the ribonuclease gene superfamily
37. An encyclopedia of mouse genes
38. dbEST-database for expressed sequence tags
39. Characterization and sequencing of rabbit, pig and mouse angiogenins: Discernment of functionally important residues and regions
40. Characterization of mouse angiogenin-related protein: Implications for functional studies on angiogenin
41. mAngiogenin-3, a target gene of oncoprotein E2a-Pbx1, encodes a new angiogenic member of the angiogenin family
42. Identification of functional arginines in human angiogenin by site-directed mutagenesis
43. The angiogenins
44. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
45. How to measure and predict the molar absorption coefficient of a protein
46. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
47. Ribonucleolytic activity of angiogenin: Essential histidine, lysine, and arginine residues
48. Low-usage codons in Escherichia coli, yeast, fruit fly and primates
49. Studies on the reduction and re-formation of protein disulfide bonds
50. Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers
51. Chemical synthesis of a gene coding for human angiogenin, its expression in Escherichia coli and conversion of the product into its active form
52. Ribonuclease variants with potent cytotoxic activity
53. ab-fragments produced in Escherichia coli
54. Expression and characterization of recombinant human eosinophil-derived neurotoxin and eosinophil-derived neurotoxin-anti-transferrin receptor sFv
55. Role of the N terminus in RNase A homologues: Differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity
56. Kinetic and product distribution analysis of human eosinophil cationic protein indicates a subsite arrangement that favours exonuclease-type activity
57. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product
58. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
59. Cis proline mutants of ribonuclease A. I. Thermal stability
60. Bovine seminal ribonuclease produced from a synthetic gene
61. A study of the intracellular routing of cytotoxic ribonucleases
62. An angiogenic protein from bovine serum and milk: Purification and primary structure of angiogenin-2