Isolation, culture, characterization and use of human renal tubular epithelial cells

Journal of Nephrology

Volumn 14, Issue 3, 2001, Pages 204-210

  Van Kooten, C ^a   Lam, S ^a   Daha, M R ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

In vitro culture; Inflammation; Kidney; Proximal tubular epithelial cells

Indexed keywords

CD30 ANTIGEN; CD40 ANTIGEN; CELL SURFACE MARKER; THY 1 ANTIGEN; UNCLASSIFIED DRUG;

ANTIGEN EXPRESSION; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL LINE; CELL TYPE; CULTURE MEDIUM; CYTOLOGY; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; INFLAMMATION; KIDNEY; KIDNEY DISEASE; KIDNEY INTERSTITIUM; KIDNEY TUBULE CELL; KIDNEY TUBULE EPITHELIUM; MORPHOLOGY; WATER TRANSPORT;

CELL CULTURE TECHNIQUES; CELL LINE; EPITHELIAL CELLS; FIBROBLASTS; HUMANS; KIDNEY TUBULES; UROTHELIUM;

EID: 0034956189     PISSN: 11218428     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (59)

1. The progression of renal disease
2. Tubulointerstitial changes as a major determinant in the progression of renal damage
3. Understanding the nature of renal disease progression
4. Early molecular mechanisms in the progression of renal failure: Role of growth factors and protooncogenes
5. The role of tubulointerstitial injury in chronic renal failure
6. Cellular and molecular mediators in common pathway mechanisms of chronic renal disease progression
7. Isolation, culture and characterization of human renal tubular cells
8. Analysis and isolation of renal tubular cells by flow cytometry
9. Characterization of pure proximal and heterogeneous distal human tubular cells in culture
10. Isolation of proximal and distal tubule cells from human kidney by immunomagnetic separation
11. Defined human renal tubular epithelia in culture: Growth, characterization, and hormonal response
12. Tissue culture of human kidney epithelial cells of proximal tubule origin
13. Growth of functional primary cultures of kidney epithelial cells in defined medium
14. Characterization of primary cell cultures derived from rat renal proximal tubules
15. Normal rat kidney proximal tubule cells in primary and multiple subcultures
16. Biochemical, functional, and morphological characterization of a primary culture of rabbit proximal tubule cells
17. Consecutive use of hormonally defined serum-free media to establish highly differentiated human renal proximal tubule cells in primary culture
18. Polarity of stimulation and secretion of transforming growth factor-β-1 by cultured proximal tubular cells
19. Fibronectin production by human tubular cells: The effect of apical protein
20. Apical proteins stimulate complement synthesis by cultured human proximal tubular epithelial cells
21. Induction of monocyte chemoattractant protein-1 in proximal tubule cells by urinary protein
22. Protein overload stimulates RANTES production by proximal tubular cells depending on NF-kB activation
23. Epidermal growth factor or transforming growth factor alpha is required for kidney tubulogenesis in matrigel cultures in serum-free medium
24. Modulation of cell differentiation in perfusion culture
25. Flow cytometric immunodissection of the human nephron in vivo and in vitro
26. Immunodissection of the human proximal nephron: Flow sorting of S1S2S3, S1S2 and S3 proximal tubular cells
27. Stage- and segment-specific expression of cell-adhesion molecules N-CAM, A-CAM, and L-CAM in the kidney
28. Transdifferentiation of distal but not proximal tubular epithelial cells from human kidney in culture
29. Renal proximal tubular epithelium from patients with nephropathic cystinosis: Immortilized cell lines as in vitro model systems
30. Production of inflammatory mediators and cytokine responsiveness of an SV40-transformed human proximal tubular epithelial cell line
31. HK-2: An immortalized proximal tubule epithelial cell line from normal adult human kidney
32. Cyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures
33. Increased proximal tubular cholesterol content: Implications for cell injury and "acquired cytoresistance"
34. Calcitriol directly sensitizes renal tubular cells to ATP-depletion- and iron-mediated attack
35. C3a is made by proximal tubular HK-2 cells and activates them via the C3a receptor
36. In vitro culture of cells exfoliated in the urine by patients with diabetes mellitus
37. Escherichia coli induces transuroepithelial neutrophil migration by an intercellular adhesion molecule-1-dependent mechanism
38. Characterization of human renal fibroblasts in health and disease: I. Immunophenotyping of cultured tubular epithelial cells and fibroblasts derived from kidneys with histologically proven interstitial fibrosis
39. Human renal fibroblast cell lines (tFKIF and tNKF) are new tools to investigate pathophysiologic mechanisms of renal interstitial fibrosis
40. Transdifferentiation: A new angle on renal fibrosis
41. Tubular epithelial-myofibroblast transdifferentiation in progressive tubulointerstitial fibrosis in 5/6 nephrectomized rats
42. Identification and characterization of a fibroblast marker: FSP1
43. Differential expression of cell-cell adhesion proteins and cyclin D in MEK1-transdifferentiated MDCK cells
44. Early role of Fsp1 in epithelial-mesenchymal transformation
45. Transforming growth factor-beta regulates tubular epithelial-myofibroblasts transdifferentiation in vitro
46. Mesenchymal to epithelial conversion in rat metanephros is induced by LIF
47. CD26, let it cut or cut it down
48. Cells isolated from the human cortical interstitium resemble myofibroblasts and bind neutrophils in an ICAM-1 -dependent manner
49. Novel fibroblast-specific monoclonal antibodies: Properties and specificities
50. The monoclonal antibody AS02 recognizes a protein on human fibroblasts being highly homologous to Thy-1
51. Tubular epithelial cells: A critical cell type in the regulation of renal inflammatory processes
52. Immunological function of tubular epithelial cells, the functional implications of CD40 expression
53. IL-4 and IL-13 augment cytokine-and CD40-induced RANTES production by human renal tubular epithelial cells in vitro
54. Induction of RANTES, HLA-DR, and intercellular adhesion molecule-1 on highly purified distal tubular cells from human kidney
55. Transforming growth factor beta and angiotensin II: The missing link from glomerular hyperfiltration to glomerulosclerosis
56. Molecular mechanisms of angiotensin II in the kidney: Emerging role in the progression of renal disease: Beyond haemodynamics
57. Amino-terminal truncation of chemokines by CD26/dipeptidyl-peptidase IV
58. Cleavage by CD26/dipeptidyl peptidase IV converts the chemokine LD78beta into a most efficient monocyte attractant and CCR1 agonist
59. Inhibition of CD26/dipeptidyl peptidase IV activity in vivo prolongs cardiac allograft survival in rat recipients