Single-step method for purification of Shiga toxin-1 B subunit using receptor-mediated affinity chromatography by globotriaosylceramide-conjugated octyl sepharose CL-4B

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 267-275

  Nakajima, Hideki ^a,c   Katagiri, Yohko U ^a   Kiyokawa, Nobutaka ^a   Taguchi, Tomoko ^a   Suzuki, Toyo ^a   Sekino, Takaomi ^a   Mimori, Kenichi ^a   Saito, Masahiro ^a   Nakao, Hiroshi ^b   Takeda, Tae ^b   Fujimoto, Junichiro ^a  

^a Department of Pathology ^*  (Japan)

^b NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; BACTERIAL PROTEIN; CARRIER PROTEIN; ESCHERICHIA COLI; GLOBOTRIAOSYLCERAMIDE; PROTEIN CONFORMATION; PROTEIN PURIFICATION; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; SEPHAROSE; SHIGA TOXIN;

ESCHERICHIA COLI;

EID: 0034953596     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1449     Document Type: Article

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