The RNA-N-glycosidase activity of Shiga-like toxin I: Kinetic parameters of the native and activated toxin

Toxicon

Volumn 35, Issue 9, 1997, Pages 1431-1437

  Brigotti, Maurizio ^a   Carnicelli, Domenica ^a   Alvergna, Paola ^a   Mazzaracchio, Raffaella ^a   Sperti, Simonetta ^a   Montanaro, Lucio ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

RIBONUCLEASE; SHIGA TOXIN;

ALPHA CHAIN; ANIMAL TISSUE; ARTICLE; BINDING SITE; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; DRUG ACTIVITY; DRUG ANALYSIS; DRUG TOXICITY; ENZYME ACTIVATION; ENZYME ACTIVITY; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN SYNTHESIS; RABBIT; REACTION ANALYSIS; RETICULOCYTE LYSATE; RIBOSOME;

ANIMALS; BACTERIAL TOXINS; DITHIOTHREITOL; KINETICS; N-GLYCOSYL HYDROLASES; RABBITS; SHIGA-LIKE TOXIN I; TRYPSIN; UREA;

ANIMALIA; ARTEMIA SALINA; FELIS CATUS; ORYCTOLAGUS CUNICULUS; SALINA;

EID: 0030721959     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(96)00225-5     Document Type: Article

References (21)

1. Barbieri, L., Battelli, M. G. and Stirpe, F. (1993) Ribosome-inactivating proteins from plants. Biochimica et Biophysica Acta 1154, 237-282.
2. Brigotti, M., Rambelli, F., Zamboni, M., Montanaro, L. and Sperti, S. (1989) Effect of α-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes. Biochemical Journal 257, 723-727.
3. Brigotti, M., Carnicelli, D., Sperti, S. and Montanaro, L. (1994) RNA present in post-ribosomal supernatants makes ribosomes susceptible to inactivation by gelonin and α-sarcin. Biochemistry and Molecular Biology International 32, 585-596.
4. Trp is required for ribosomes inactivation by gelonin, a plant RNA-N-glycosidase. Biochemical Journal 310, 249-253.
5. Brigotti, M., Carnicelli, D., Alvergna, P., Pallanca, A., Sperti, S. and Montanaro, L. (1995b) Differential up-regulation by tRNAs of ribosome-inactivating proteins. FEBS Letters 373, 115-118.
6. Brown, J. E., Ussery, M. A., Leppla, S. H. and Rothman, S. W. (1980) Inhibition of protein synthesis by Shiga toxin. Activation of the toxin and inhibition of peptide elongation. FEBS Letters 117, 84-88.
7. Burgess, B. J. and Roberts, L. M. (1993) Proteolytic cleavage at arginine residues within the hydrophilic disulphide loop of Escherichia coli Shiga-like toxin I A subunit is not essential for cytotoxicity. Molecular Microbiology 10, 171-179.
8. Donohue-Rolfe, A., Jacewicz, M. and Keusch, G. T. (1988) Shiga toxin as inhibitor of protein synthesis. Methods in Enzymology 165, 231-235.
9. Endo, Y. and Tsurugi, K. (1988) The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. Journal of Biological Chemistry 263, 8735-8739.
10. Fraker, P. J. and Speck, J. C. Jr. (1978) Protein and cell membrane iodinations with a sparingly soluble chloroamide, l,3,4,6-tetrachloro-3a,6a-diphenylglycoluril. Biochemical and Biophysical Research Communications 80, 849-857.
11. Garred, Ø., van Deurs, B. and Sandvig, K. (1995) Furin-induced cleavage and activation of Shiga toxin. Journal of Biological Chemistry 270, 10817-10821.
12. O'Brien, A. D., Tesh, V. L., Donohue-Rolfe, A., Jackson, M. P., Olsnes, S., Sandvig, K., Lindberg, A. A. and Keusch, G. T. (1992) Shiga toxin: biochemistry, genetics, mode of action and role in pathogenesis. Current Topics in Microbiology and Immunology 180, 65-94.
13. Olsnes, S., Reisbig, R. and Eiklid, K. (1981) Subunit structure of Shigella cytotoxin. Journal of Biological Chemistry 256, 8732-8738.
14. Reisbig, R., Olsnes, S. and Eiklid, K. (1981) The cytotoxic activity of Shigella toxin. Evidence for catalytic inactivation of the 60 S ribosomal subunit. Journal of Biological Chemistry 256, 8739-8744.
15. Ryd, M., Alfredsson, H., Blomberg, L., Anderson, Å. and Lindberg, A. A. (1989) Purification of Shiga toxin by α-D-galactose-(1→4)-β-D-galactose-(l→4)-D-glucose (1→) receptor ligand-based chromatography. FEBS Letters 258, 320-322.
16. 2 subunits is not required for toxin activity. Journal of Biological Chemistry 269, 4853-4859.
17. Sperti, S., Brigotti, M., Zamboni, M., Carnicelli, D. and Montanaro, L. (1991) Requirements for the inactivation of ribosomes by gelonin. Biochemical Journal 277, 281-284.
18. Strockbine, N. A., Jackson, M. P., Sung, L. M., Holmes, R. K. and O'Brien, A. D. (1988) Cloning and sequencing of the genes of Shiga toxin from Shigella dysenteriae type I. Journal of Bacteriology 170, 1116-1122.
19. Surewicz, W. K., Surewicz, K., Mantsch, H. H. and Auclair, F. (1989) Interaction of Shigella toxin with globotriaosyl ceramide receptors-containing membranes: a fluorescent study. Biochemical and Biophysical Research Communications 160, 126-132.
20. Yutsudo, T., Honda, T., Miwatani, T. and Takeda, Y. (1987) Physicochemical characterization of A and B subunits of Shiga toxin and reconstitution of holotoxin from isolated subunits. Microbiology and Immunology 31, 189-197.
21. Zamboni, M., Brigotti, M., Rambelli, F., Montanaro, L. and Sperti, S. (1989) High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin. Biochemical Journal 259, 639-643.