A simple assay method for bacterial binding to glycosphingolipids on a polyvinylidene difluoride membrane after thin-layer chromatography blotting and in situ mass spectrometric analysis of the ligands

Analytical Biochemistry

Volumn 236, Issue 1, 1996, Pages 35-40

  Isobe, Tomomi ^a,b   Naiki, Masaharu ^c   Handa, Shizuo ^a   Taki, Takao ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; FATTY ACIDS; LIGANDS; MASS SPECTROMETERS; MASS SPECTROMETRY; THIN LAYER CHROMATOGRAPHY;

BACTERIAL BINDING; GLYCOSPHINGOLIPIDS; HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY; HYDROXY FATTY ACIDS; MASS SPECTROMETRIC ANALYSIS; N-GLYCOLYLNEURAMINIC ACID; PHOSPHATE-BUFFERED SALINES; POLYVINYLIDENE DIFLUORIDE;

MEMBRANES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

CASEIN; CERAMIDE; GANGLIOSIDE GM2; GANGLIOSIDE GM3; GLYCOSPHINGOLIPID; HYDROXY FATTY ACID; METHIONINE; N ACETYLNEURAMINYLPARAGLOBOSIDE; N GLYCOLOYLNEURAMINIC ACID; PARAGLOBOSIDE; POLYVINYL DERIVATIVE; POLYVINYLIDENE DIFLUORIDE; POLYVINYLIDENE FLUORIDE; SIALYLPARAGLOBOSIDE; SODIUM CHLORIDE; SULFUR 35; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; ASSAY; BACTERIUM ADHERENCE; CONTROLLED STUDY; ESCHERICHIA COLI; FIMBRIA; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEMBRANE; NONHUMAN; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY;

EID: 0029943763     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0128     Document Type: Article

References (20)

1. Kallenius, G., Mollby, R., Svenson, S. B., Weinberg, J., Lundblad, S., and Svensson, S. (1980) FEMS Microbiol. Lett. 7, 297-301.
2. Korhonen, T. K., Valtonen, M. V., Parkkinen, J., Vaisanen-Rhen, V., Finne, J., Orskov, F., Orskov, I., Svenson, S. B., and Makela, P. H. (1985) Infect. Immun. 48, 486-491.
3. Smit, H., Gaastra, W., Kamerling, J. P., Vliegenthart, J. F., and De Graaf, F. K. (1984) Infect. Immun. 46, 578-584.
4. Teneberg, S., Willemsen, P., De Graaf, F. K., and Karlsson, K.-A. (1990) FEBS Lett. 263, 10-14.
5. Hansson, G. C., Karlsson, K.-A., Larsson, G., Stromberg, N., and Thurin, J. (1985) Anal. Biochem. 146, 158-163.
6. Karlsson, K.-A., and Stromberg, N. (1987) Methods Enzymol. 138, 220-232.
7. Kyogashima, M., Ginsburg, V., and Krivan, H. C. (1989) Arch. Biochem. Biophys. 270, 391-397.
8. Stromberg, N., and Karlsson, K.-A. (1990) J. Biol. Chem. 265, 11251-11258.
9. Prasadarao, N. V., Wass, C. A., Hacker, J., Jann, K., and Kim, K. S. (1993) J. Biol. Chem. 268, 10356-10363.
10. Taki, T., Handa, S., and Ishikawa, D. (1994) Anal. Biochem. 221, 312-316.
11. Taki, T., Kasama, T., Handa, S., and Ishikawa, D. (1994) Anal. Biochem. 223, 232-238.
12. Yuyama, Y., Yoshimatsu, K., Ono, E., Saito, M., and Naiki, M. (1993) J. Biochem. 113, 488-492.
13. Taki, T., Matsuo, K., Yamamoto, K., Matsubara, T., Hayashi, A., Abe, T., and Matsumoto, M. (1988) Lipids 23, 192-198.
14. Ono, E., Abe, K., Nakazawa, M., and Naiki, M. (1989) Infect. Immun. 57, 907-911.
15. De Graaf, F. K., Wientjes, F. B., and Klaasen-Boor, P. (1980) Infect. Immun. 27, 216-221.
16. Taki, T., Ishikawa, D., Handa, S., and Kasama, T. (1995) Anal. Biochem. 225, 24-27.
17. Teneberg, S., Willemsen, P. T., De Graaf, F. K., and Karlsson, K.-A. (1993) FEMS Microbiol. Lett. 109, 107-112.
18. Karlsson, K.-A. (1989) Annu. Rev. Biochem. 58, 309-350.
19. Bock, K., Breimer, M. E., Brignole, A., Hansson, G. C., Karlsson, K.-A., Larson, G., Leffler, H., Samuelsson, B. E., Stromberg, N., Eden, C. S., and Thurin, J. (1985) J. Biol. Chem. 260, 8545-8551.
20. Kannagi, R., Stroup, R., Cochran, N. A., Urdal, D. L., Young, W. W., and Hakomori, S. (1983) Cancer. Res. 43, 4997-5005.