Real-time PCR used to measure stress-induced changes in the expression of the genes of the alginate pathway of Pseudomonas aeruginosa

Journal of Applied Microbiology

Volumn 91, Issue 1, 2001, Pages 29-37

  Edwards, K J ^a   Saunders, N A ^a  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENE EXPRESSION; POLYMERASE CHAIN REACTION;

BACTERIAL GENES; HEATSHOCKS; MRNA LEVELS; NORMAL CONDITION; PSEUDOMONAS AERUGINOSA; QUANTITATIVE RESULT; REAL-TIME PCR; REGULATORY GENES; STRESS CONDITION; STRESS-INDUCED;

ALGINATE;

ALCOHOL; ALGINIC ACID; MESSENGER RNA;

ALGD GENE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; GENE EXPRESSION; GENE MUTATION; HEAT SHOCK; MUCA GENE; MUCB GENE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; REGULATOR GENE; STRESS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0034943741     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2001.01339.x     Document Type: Article

References (28)

1. Pseudomonas aeruginosa infection in cystic fibrosis: Molecular approaches to a medical problem
2. Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa
3. The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes
4. Gene algD coding for GDP mannose dehydrogenase is transcriptionally activated in mucoid Pseudomonas aeruginosa
5. Mucoid Pseudomonas aeruginosa in cystic fibrosis: Mutations in the muc loci affect transcription of the algR and algD genes in response to environmental stimuli
6. Pulmonary dehydration and infection in cystic fibrosis: Evidence that ethanol activates alginate gene expression and induction of mucoidy in Pseudomonas aeruginosa
7. Production and characterization of the slime polysaccharide of Pseudomonas aerugiuosa
8. Fundamentals of DNA hybridisation arrays for gene expression analysis
9. Quantitative RT-PCR: Pitfalls and potential
10. Pseudomonas aeruginosa AlgB, which modulates the expression of alginate, is a member of the NtrC subclass of prokaryotic regulators
11. Antibiotic interaction and diffusion through alginate and exopolysaccharide of cystic fibrosis-derived Pseudomonas aeruginosa
12. Alginate biosynthesis and other unusual haracteristics associated with the pathogenesis of Pseudomonas aeruginosa in cystic fibrosis
13. Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia
14. Microbiology of cystic fibrosis
15. Chromosomal genetics of Pseudomonas
16. Pathogenesis of cystic fibrosis
17. Pseudomonas - An attempt at a general classification
18. Differentiation of Pseudomonas aeruginosa into the alginate-producing form: Inactivation of mucB causes conversion to mucoidy
19. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients
20. Alginate synthesis by Pseudomonas aeruginosa: A key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients
21. The penetration of antibiotics into aggregates of mucoid and non-mucoid Pseudomonas aeruginosa
22. Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: Current status of the host-bacterium interaction
23. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
24. Multiple promoters and induction by heat shock of the gene encoding the alternative sigma factor AlgU (sigma E) which controls mucoidy in cystic fibrosis isolates of Pseudomonas aeruginosa
25. Control of AlgU, a member of the sigma E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis
26. Development of a sensitive reverse transcriptase PCR assay, RT-RPCR, utilizing rapid cycle times
27. Continuous fluorescence monitoring of rapid cycle DNA amplification
28. The LightCyclerTM: A microVolume multisample fluorimeter with rapid temperature control