Control of AlgU, a member of the σ(E)-like family of stress sigma factors, by the negative regulators mucA and mucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis

Journal of Bacteriology

Volumn 178, Issue 16, 1996, Pages 4997-5004

  Schurr, M J ^a,b   Yu, H ^a,b   Martinez Salazar, J M ^a,c   Boucher, J C ^a,b   Deretic, V ^a,b  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

SIGMA FACTOR;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIUM MUTANT; CYSTIC FIBROSIS; GENE MUTATION; NONHUMAN; OXIDATIVE STRESS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0029747226     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.16.4997-5004.1996     Document Type: Article

References (49)

1. Alper, S., L. Duncan, and R. Losick. 1994. An adenosine nucleotide switch controlling the activity of a cell type-specific transcription factor in B. subtilis. Cell 77:195-205.
2. Bass, S., Q. Gu, and A. Christen. 1996. Multicopy suppressors of Prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB). DksA, and a truncated RlpA. J. Bacteriol. 178:1154-1161.
3. Benson, A. K., and W. G. Haldenwang. 1993. Bacillus subtilis sigma B is regulated by a binding protein (RsbW) that blocks its association with core polymerase. Proc. Natl. Acad. Sci. USA 90:2230-2234.
4. Boucher, J. C., J. Martinez-Salazar, M. J. Schurr, M. H. Mudd, H. Vu, and V. Deretic. 1996. Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA. J. Bacteriol. 178:511-523.
5. Boucher, J. C., H. Yu, M. J. Schurr, and V. Deretic. Unpublished observations.
6. Cheng, K.-J., J. M. Ingram, and J. W. Costerton. 1970. Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. J. Bacteriol. 104:748-753.
7. Chi, E., and D. H. Bartlett. 1995. An rpoE-like locus controls outer membrane protein synthesis and growth at cold temperatures and high pressures in the deep sea bacterium Photobacterium sp. strain SS9. Mol. Microbiol. 17:713-726.
8. Deretic, V., S. Chandrasekharappa, J. F. Gill, D. K. Chatterjee, and A. M. Chakrabarty. 1987. A set of cassettes and improved vectors for genetic and biochemical characterization of Pseudomonas aeruginosa. Gene 57:61-72.
9. Deretic, V., M. J. Schurr, J. C. Boucher, and D. W. Martin. 1994. Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J. Bacteriol. 176:2773-2780.
10. Deretic, V., M. J. Schurr, and H. Vu. 1995. Pseudomonas aeruginosa, mucoidy and the chronic infection phenotype in cystic fibrosis. Trends Microbiol. 3:351-356.
11. DeVries, C. A., and D. E. Ohman. 1994. Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation. J. Bacteriol. 176:6677-6687.
12. E from Bacillus subtilis. Proc. Natl. Acad. Sci. USA 90:2325-2329.
13. Erickson, J. W., and C. A. Gross. 1989. Identification of the subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression. Genes Dev. 3:1462-1471.
14. Fleishmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J.-F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKeenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L.-I. Lieu, A. Glodek, J. M. Kelley, J. F. Weidman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Georghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
15. Fyfe, J. A. M., and J. R. W. Govan. 1980. Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. J. Gen. Microbiol. 119:443-450.
16. 32. Cell 69:833-842.
17. Gilligan, P. H. 1991. Microbiology of airway disease in patients with cystic fibrosis. Clin. Microbiol. Rev. 4:35-51.
18. Goldberg, J. B., W. L. Gorman, J. L. Flynn, and D. E. Ohman. 1993. A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species. J. Bacteriol. 175:1303-1308.
19. Gorham, H. C., S. J. McGowan, P. R. H. Robson, and D. A. Hodgson. 1996. Light-induced carotenogenesis in Myxococcus xanthus: light-dependent membrane sequestration of ECF sigma factor CarQ by anti-sigma factor CarR. Mol. Microbiol. 19:171-186.
20. Hager, D. A., and R. R. Burgess. 1980. Elution of proteins from sodium dodecyl sulfate polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase and other enzymes. Anal. Biochem. 109:76-86.
21. Hancock, R. E. W., and H. Nikaido. 1978. Outer membranes of gramnegative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J. Bacteriol. 136:381-390.
22. E). Proc. Natl. Acad. Sci. USA 92:7941-7945.
23. Hughes, K. T., K. L. Gillen, M. J. Semon, and J. E. Karlinsey. 1993. Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator. Science 262:1277-1280.
24. Kessler, E., and M. Safrin. 1988. Synthesis, processing, and transport of Pseudomonas aeruginosa elastase. J. Bacteriol. 170:5241-5247.
25. Knutson, C. A., and A. Jeanes. 1976. A new modification of the carbazole reaction: application to heteropolysaccharides. Anal. Biochem. 24:470-481.
26. 32-independent mechanism of heat-inducible transcription. Nucleic Acids Res. 16:10053-10067.
27. Lonetto, M. A., K. L. Brown, K. E. Rudd, and J. M. Buttner. 1994. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase σ factors involved in the regulation of extracytoplasmic functions. Proc. Natl. Acad. Sci. USA 91:7573-7577.
28. Lowe, P. A., D. A. Hager, and R. R. Burgess. 1979. Purification and properties of the cr subunit of Escherichia coli DNA-dependent RNA polymerase. Biochemistry 18:1344-1352.
29. Martin, D. W., B. W. Holloway, and V. Deretic. 1993. Characterization of a locus determining the mucoid status of Pseudomonas aeruginosa: AlgU shows sequence similarities with a Bacillus sigma factor. J. Bacteriol. 175:1153-1164.
30. Martin, D. W., M. J. Schurr, M. H. Mudd, and V. Deretic. 1993. Differentiation of Pseudomonas aeruginosa into the alginatc-producing form: inactivation of mucB causes conversion to mucoidy. Mol. Microbiol. 9:495-506.
31. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. W. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of the conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90:8377-8381.
32. E and stress response. J. Bacteriol. 176:6688-6696.
33. Martinez-Salazar, J. M., S. Moreno, R. Nájera, J. C. Boucher, G. Espín, G. Soberón-Chávez, and V. Deretic. 1996. Characterization of the genes coding for the putative sigma factor AlgU and its regulators MucA, MucB, MucC, and MucD in Azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. J. Bacteriol. 178:1800-1808.
34. McGowan, S. J., H. C. Gorham, and D. A. Hodgson. 1993. Light induced carotenogenesis in Myxococcus xanthus: DNA sequence analysis of the carR region. Mol. Microbiol. 10:713-735.
35. F an Escherichia coli heat-inducible σ-factor, is modulated by expression of outer membrane proteins. Gen. Dev. 7:2618-2628.
36. F. Mol. Microbiol. 6:3149-3157.
37. Oi, V. T., and L. A. Herzenberg. 1980. Immunoglobulin-producing hybrid cell lines, p. 351-372. In B. B. Mishell and S. M. Shiigi (ed.), Selected methods in cellular immunology. W. H. Freeman & Co., San Francisco.
38. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
39. 24) heat shock sigma factor of Escherichia coli. EMBO J. 14:1043-1055.
40. E, in Escherichia coli. EMBO J. 14:1032-1042.
41. Schurr, M. J., D. W. Martin, M. H. Mudd, and V. Deretic. 1994. Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy. J. Bacteriol. 176:3375-3382.
42. E) which controls mucoidy in cystic fibrosis isolates of Pseudomonas aeruginosa. J. Bacteriol. 177:5670-5679.
43. Schurr, M. J., H. Yu, J. M. Martinez-Salazar, N. S. Hibler, and V. Deretic. 1995. Biochemical characterization and posttranslational modification of AlgU, a regulator of stress response in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 216:874-880.
44. Strauch, K. L., and J. Beckwith. 1988. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. USA 85:1576-1580.
45. Studier, F. W., and B. A. Moffat. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
46. Waller, P. R. H., and R. T. Sauer. 1996. Characterization of degQ and degS. Escherichia coli genes encoding homologs of the DegP protease. J. Bacteriol. 178:1146-1153.
47. Xiao, Y., S. Heu, J. Yi, Y. Lu, and S. W. Hutcheson. 1994. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J. Bacteriol. 176:1025-1036.
48. Yu, H., M. J. Schurr, J. C. Boucher, J. M. Martinez-Salazar, D. W. Martin, and V. Deretic. 1996. Molecular mechanism of conversion to mucoidy in Pseudomonas aeruginosa, p. 384-397. In T. Nakazawa, K. Furukawa, D. Haas, and S. Silver (ed.), Molecular biology of pseudomonads. ASM Press, Washington, D.C.
49. E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa. J. Bacteriol. 177:3259-3268.