Lipopolysaccharide (LPS) labeled with alexa 488 hydrazide as a novel probe for LPS binding studies

Cytometry

Volumn 41, Issue 4, 2000, Pages 316-320

  Triantafilou, Kathy ^a   Triantafilou, Martha ^a   Fernandez, Nelson ^a  

^a UNIVERSITY OF ESSEX   (United Kingdom)

Author keywords

Alexa hydrazide; Inflammatory responses; Lipopolysaccharide

Indexed keywords

ALDEHYDE; ALEXA 488 HYDRAZIDE; BACTERIUM LIPOPOLYSACCHARIDE; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; GALACTOSE; GALACTOSE OXIDASE; HYDRAZIDE; OLIGOSACCHARIDE; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CARBOHYDRATE ANALYSIS; CONCENTRATION RESPONSE; CYTOKINE PRODUCTION; FLOW CYTOMETRY; FLUORESCENCE; IMAGING; INFLAMMATION; METHODOLOGY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN INDUCTION, SYNTHESIS AND MODIFICATION;

ANIMALS; ANTIGENS, CD14; CHO CELLS; CRICETINAE; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; GALACTOSE OXIDASE; HYDRAZINES; LIPOPOLYSACCHARIDES; PROTEIN BINDING; SALMONELLA; SENSITIVITY AND SPECIFICITY; TRANSFECTION; TUMOR NECROSIS FACTOR-ALPHA;

ALEXA; ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0034541237     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20001201)41:4<316::AID-CYTO10>3.0.CO;2-Z     Document Type: Article

References (17)

1. Septic shock pathogenesis
2. Recognition of endotoxin by cells leading to transmembrane signaling
3. Human Toll-like receptor 2 confers responsiveness to bacterial lipopolysaccharide
4. Lipopolysaccharide receptor on rabbit peritoneal macrophages. I. Binding characteristics
5. Characteristics of lipopolysaccharide interaction with human peripheral-blood monocytes
6. Lipopolysaccharide complexed with soluble CD14 binds to normal human monocytes
7. Saturable CD14-dependent binding of fluorescein-labelled lipopolysaccharide to human monocytes
8. Rough and smooth forms of fluorescein-labelled bacterial endotoxin exhibit CD14/LBP dependent and independent binding that is influenced by endotoxin concentration
9. Innate immune recognition of bacterial lipopolysaccharide: Dependence on interactions with membrane lipids and endocytic movement
10. Serum independent binding of lipopolysaccharide to human monocytes is trypsin sensitive and does not involve CD14
11. Control of lipopolysaccharide (LPS) binding and LPS-induced tumor necrosis factor secretion in human peripheral-blood monocytes
12. Binding of rough lipopolysaccharides (LPS) to human leukocytes. Inhibition by anti-LPS monoclonal antibodies
13. A region of human CD14 required for lipopolysaccharide binding
14. Dual effects of soluble CD14 on LPS priming of neutrophils
15. A method to detect 2-keto-3-deoxyoctant and related compounds on pherograms and chromatograms
16. Structural studies on the hexose region of the core in lipopolysaccharides from Enterobacteriaceae
17. Endotoxaemia: An early predictor of septicaemia in febrile patients