Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: A quantifiable method for gene knockdown

Genesis

Volumn 30, Issue 3, 2001, Pages 154-156

  Draper, Bruce W ^a   Morcos, Paul A ^b   Kimmel, Charles B ^a  

^a UNIVERSITY OF OREGON   (United States)

^b Gene Tools LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; EXON; INTRON; KNOCKOUT GENE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; ZEBRA FISH;

ANIMALS; FIBROBLAST GROWTH FACTOR 8; FIBROBLAST GROWTH FACTORS; GENE EXPRESSION REGULATION; MORPHOLINES; MUTATION; OLIGONUCLEOTIDES, ANTISENSE; PHENOTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA SPLICE SITES; RNA SPLICING; RNA, MESSENGER; ZEBRAFISH; ZEBRAFISH PROTEINS;

ANIMALIA; DANIO RERIO; VERTEBRATA;

EID: 0034904642     PISSN: 1526954X     EISSN: None     Source Type: Journal    
DOI: 10.1002/gene.1053     Document Type: Article

References (7)

1. Morphant: A new systematic vertebrate functional genomics approach
2. A role for fgf-8 in the dorsoventral patterning of the zebrafish gastrula
3. Autoradiography using storage phosphor technology
4. The zebrafish midblastula transition
5. A simple method for delivering morpholino antisense oligos into the cytoplasm of cells
6. Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis
7. Antisense oligonucleotides with different backbones: Modification of the splicing pathway and efficacy of uptake