An aromatic, but not a basic, residue is involved in the toxicity of group-II phospholipase A2 neurotoxins

Biochemical Journal

Volumn 341, Issue 1, 1999, Pages 139-145

  Pungerčar, Jože ^a   Križaj, Igor ^a   Liang, Ning Sheng ^b   Gubenšek, Franc ^a,c  

^a JO EF STEFAN INSTITUTE   (Slovenia)

^b GUANGXI MEDICAL UNIVERSITY   (China)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Acceptor binding affinity; Ammodytoxin; Enzyme activity; Protein engineering; Vipera ammodytes ammodytes

Indexed keywords

AMMODYTOXIN A; NEUROTOXIN; PHOSPHOLIPASE A2; SNAKE VENOM;

ARTICLE; BINDING AFFINITY; BRAIN; CARBOXY TERMINAL SEQUENCE; CATTLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; MUTANT; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATTLE; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NEUROTOXINS; PHENYLALANINE; PHOSPHOLIPASES A; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SYNAPTIC MEMBRANES; TOXICITY TESTS; VIPER VENOMS;

AMMODYTES; BOS TAURUS; BOVINAE; ESCHERICHIA COLI; SERPENTES; VIPERA; VIPERA AMMODYTES AMMODYTES;

EID: 2442724483     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3410139     Document Type: Article

References (50)

1. Thouin, Jr., L. G., Ritonja, A., Gubenšek, F. and Russel, F. E. (1982) Toxicon 20, 1051-1058
2. Chang, C. C. and Su, M. J. (1980) Toxicon 18, 641-648
3. Scott, V. E., Parcej, D. N., Keen, J. N., Findlay, J. B. and Dolly, J. O. (1990) J. Biol. Chem. 265, 20094-20097
4. Schlimgen, A. K., Helms, J. A., Vogel, H. and Perin, M. S. (1995) Neuron 14, 519-526
5. Dodds, D. C., Omeis, I. A., Cushman, S. J., Helms, J. A. and Perin, M. S. (1997) J. Biol. Chem. 272, 21488-21494
6. Lambeau, G., Barhanin, J., Schweitz, H., Qar, J. and Lazdunski, M. (1989) J. Biol. Chem. 264, 11503-11510
7. Jeng, T. W. and Fraenkel-Conrat, H. (1978) FEBS Lett. 87, 291-296
8. 2 Enzymes: Structure, Function and Mechanism (Kini, R. M., ed.), pp. 1-28, John Wiley, Chichester
9. Kelley, M. J., Crowl, R. M. and Dennis, E. A. (1992) Biochim. Biophys. Acta 1118, 107-115
10. Lathrop, B. K., Burack, W. R., Biltonen, R. L. and Rule, G. S. (1992) Protein Express. Purif. 3, 512-517
11. Hodgson, D., Gasparini, S., Drevet, P., Ducancel, P., Bouet, F., Boulain, J.-C., Harris, J. B. and Menez, A. (1993) Eur. J. Biochem. 212, 441-446
12. Liang, N.-S., Pungerčar, J., Križaj, I., Štrukelj, B. and Gubenšek, F. (1993) FEBS Lett. 334, 55-59
13. Renetseder, R., Brunie, S., Dijkstra, B. W., Drenth, J. and Sigler, P. B. (1985) J. Biol. Chem. 260, 11627-11634
14. Pungerčar, J., Kordiš, D., Štrukelj, B., Liang, N.-S. and Gubenšek, F. (1991) Toxicon 29, 269-273
15. Tabor, S. (1994) in Current Protocols in Molecular Biology, vol. 2 (Ausubel, F. A., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., eds.), pp. 16.2.1-16.2.11, Greene Publishing and Wiley Interscience, New York
16. Kramer, W., Drutsa, V., Jansen, H. W., Kramer, B., Pflugfelder, M. and Fritz, H. J. (1984) Nucleic Acids Res. 12, 9441-9456
17. Norris, K., Morris, F., Christiansen, L. and Fiil, N. (1983) Nucleic Acids Res. 11, 5103-5112
18. Zoller, M. J. and Smith, M. (1987) Methods Enzymol. 154, 329-350
19. Sanger, F., Nicklen, S. and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467
20. Thannhauser, T. W. and Scheraga, H. A. (1985) Biochemistry 24, 7681-7688
21. Laemmli, U. K. (1970) Nature (London) 227, 680-685
22. Dennis, E. A. (1973) J. Lipid Res. 14, 152-159
23. Perkins, S. J. (1986) Eur. J. Biochem. 157, 169-180
24. Reed, L. J. and Muench, H. (1938) Am. J. Hygiene 27, 493-497
25. Bennett, V. Baines, A. J. and Davis, J. (1986) Methods Enzymol. 134, 55-69
26. Markwell, M. A. K., Haas, S. M., Bieber, L. L. and Tolbert, N. E. (1978) Anal. Biochem. 87, 206-210
27. Križaj, I., Dolly, J. O. and Gubenšek, F. (1994) Biochemistry 33, 13938-13945
28. Black, A. R., Breeze, A. L., Othman, I. B. and Dolly, J. O. (1986) Biochem. J. 237, 397-404
29. Tang, L., Zhou, Y.-C. and Lin, Z.-J. (1998) J. Mol. Biol. 282, 1-11
30. Ritonja, A. and Gubenšek, F. (1985) Biochim. Biophys. Acta 828, 306-312
31. Ritonja, A., Machleidt, W., Turk, V. and Gubenšek, F. (1986) Biol. Chem. Hoppe-Seyler 367, 919-923
32. Križaj, I., Ritonja, A., Turk, D. and Gubenšek, F. (1989) Biochim. Biophys. Acta 999, 198-202
33. Gowda, V. T., Schmidt, J. and Middlebrook, J. L. (1994) Toxicon 32, 665-673
34. Kasturi, S. and Gowda, T. V. (1989) Toxicon 27, 229-237
35. Tsai, I.-H., Lu, P.-J., Wang, Y.-M., Ho, C.-L. and Liaw, L.-L. (1995) Biochem. J. 311, 895-900
36. Kondo, K., Zhang, J., Xu, K. and Kagamiyama, H. (1989) J. Biochem. (Tokyo) 105, 196-203
37. Chen, Y.-C., Maraganore, J. M., Reardon, I. and Heinrikson, R. L. (1987) Toxicon 25, 401-409
38. Bouchier, C., Boulain, J.-C., Bon, C. and Menez, A. (1991) Biochim. Biophys. Acta 1088, 401-408
39. Faure, G., Harvey, A. L., Thomson, E., Saliou, B., Radvanyi, F. and Bon, C. (1993) Eur. J. Biochem. 214, 491-496
40. Viljoen, C. C., Botes, D. P. and Kruger, H. (1982) Toxicon 20, 715-737
41. Lee, C.-Y., Ho, C.-L. and Botes, D. P. (1982) Toxicon 20, 637-647
42. Mancheva, I., Kleinschmidt, T., Aleksiev, B. and Braunitzer, G. (1987) Biol. Chem. Hoppe-Seyler 368, 343-352
43. Aleksiev, B. and Shipolini, R. (1973) in Proceedings of the International Symposium on Polymers, Polimeri '73, vol. 1, pp. 417-420, Varna, Bulgaria
44. Francis, B., Bdolah, A. and Kaiser, I. I. (1995) Toxicon 33, 863-874
45. Batzri-Izraeli, R. and Bdolah, A. (1982) Toxicon 20, 867-875
46. Wang, Y.-M., Lu, P.-J., Ho, C.-L. and Tsai, I.-H. (1992) Eur. J. Biochem. 209, 635-641
47. Čurin-Šerbec, V., Novak, D., Babnik, J., Turk, D. and Gubenšek, F. (1991) FEBS Lett. 280, 175-178
48. Lugtigheid, R. B., Nicolaes, G. A., Veldhuizen, E. J., Slotboom, A. J., Verheij, H. M. and De Haas, G. H. (1993) Eur. J. Biochem. 216, 519-525
49. Scott, D. L. and Sigler, P. B. (1994) Adv. Protein Chem. 45, 53-88
50. Scott, D. L., Mandel, A. M., Sigler, P. B. and Honig, B. (1994) Biophys. J. 67, 493-504