Introduction of a C-terminal aromatic sequence from snake venom Phospholipases A2 into the porcine pancreatic isozyme dramatically changes the interfacial kinetics

European Journal of Biochemistry

Volumn 263, Issue 3, 1999, Pages 782-788

  Janssen, Marcel J W ^b   Burghout, Pieter J ^b   Verheij, Hubertus M ^b   Slotboom, Arend J ^b   Egmond, Maarten R ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b NONE

Author keywords

Calcium binding; Interfacial enzyme properties; Phospholipase A2; Site directed mutagenesis

Indexed keywords

AMPHOLYTE; CALCIUM ION; ISOENZYME; PHOSPHOLIPASE A2; PHOSPHOLIPASE A2 INHIBITOR; SNAKE VENOM;

ARTICLE; BINDING AFFINITY; CALCIUM BINDING; CARBOXY TERMINAL SEQUENCE; COMPETITIVE INHIBITION; CONTROLLED STUDY; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME POLYMORPHISM; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CALCIUM; CLONING, MOLECULAR; ESCHERICHIA COLI; ISOENZYMES; KINETICS; MICELLES; MUTAGENESIS, SITE-DIRECTED; PANCREAS; PHOSPHOLIPASES A; RECOMBINANT FUSION PROTEINS; SNAKE VENOMS; SWINE; VARIATION (GENETICS);

EID: 0011678255     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00557.x     Document Type: Article

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