Cloning, expression, and catalytic activity of Helicobacter hepaticus urease

Infection and Immunity

Volumn 69, Issue 9, 2001, Pages 5914-5920

  Beckwith, C S ^a   McGee, D J ^a   Mobley, H L T ^a   Riley, L K ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

UREASE;

ARTICLE; CLUSTER ANALYSIS; ENZYME ACTIVITY; HELICOBACTER HEPATICUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; HELICOBACTER; MICE; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; SEQUENCE ANALYSIS, DNA; UREASE;

EID: 0034874511     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.69.9.5914-5920.2001     Document Type: Article

References (55)

1. Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH
2. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
3. Inability of an isogenic urease-negative mutant stain of Helicobacter mustelae to colonize the ferret stomach
4. Detection of Helicobacter species in the liver of patients with and without primary liver carcinoma
5. Bacterial ureases in infectious diseases
6. Contribution of urease to acid tolerance in Yersinia enterocolitica
7. Helicobacter pylori
8. Structure, function and localization of Helicobacter pylori urease
9. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori
10. Enterohepatic helicobacters: Natural and experimental models
11. Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis
12. Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice
13. A novel urease-negative Helicobacter species associated with colitis and typhlitis in IL-10-deficient mice
14. Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice
15. Isolation of a novel Helicobacter species, Helicobacter cholecystus sp. nov., from the gallbladders of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis
16. Enterohepatic lesions in SCID mice infected with Helicobacter bilis
17. Enteric lesions in SCID mice infected with "Helicobacter typhlonicus", a novel urease-negative Helicobacter species
18. Helicobacter pylori glutamine synthetase lacks features associated with transcriptional and posttranslational regulation
19. Supramolecular assembly and acid resistance of Helicobacter pylori urease
20. Inflammatory large bowel disease in immunodeficient rats naturally and experimentally infected with Helicobacter bilis
21. Helicobacter pylori urease is a potent stimulus of mononuclear phagocyte activation and inflammatory cytokine production
22. Helicobacter pylori ABC transporter: Effect of allelic exchange mutagenesis on urease activity
23. Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli
24. Identification of the nik gene cluster of Brucella suis: Regulation and contribution to urease activity
25. Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity
26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
27. The Escherichia coli ATP-binding cassette (ABC) proteins
28. Cloning and expression of an immunogenic membrane-associated protein of Helicobacter hepaticus for use in an enzyme-linked immunosorbent assay
29. Isolation of Helicobacter pylori genes that modulate urease activity
30. Structural model of the nucleotide-binding conserved component of periplasmic permeases
31. Helicobacter pylori nickel-transport gene nixA: Synthesis of catalytically active urease in Escherichia coli independent of growth conditions
32. Molecular biology of microbial ureases
33. The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel
34. Serum antibodies to Helicobacter hepaticus and Helicobacter pylori in patients with chronic liver disease
35. Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491
36. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
37. Helicobacter infection and cirrhosis in hepatitis C virus carriage: Is it an innocent bystander or a troublemaker?
38. Identification of murine helicobacters by PCR and restriction enzyme analyses
39. Use of antibiotics to prevent hepatitis and typhlitis in male scid mice spontaneously infected with Helicobacter hepaticus
40. Expression of the Helicobacter pylori urel gene is required for acidic pH activation of cytoplasmic urease
41. The role of internal urease in acid resistance of Helicobacter pylori
42. Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay
43. Development of a PCR-restriction fragment length polymorphism assay using the nucleotide sequence of the Helicobacter hepaticus urease structural genes ureAB
44. The Helicobacter pylori UreI protein is not involved in urease activity but is essential for bacterial survival in vivo
45. Helicobacter pylori urease activity is toxic to human gastric epithelial cells
46. Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter
47. Helicobacter pullorum sp. nov. - Genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis
48. The complete genome sequence of the gastric pathogen Helicobacter pylori
49. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach
50. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
51. Inflammatory large bowel disease in immunodeficient mice naturally infected with Helicobacter hepaticus
52. Phenol-hypochlorite reaction for determination of ammonia
53. A H+-gated urea channel: The link between Helicobacter pylori urease and gastric colonization