Expression of the Helicobacter pylori ureI gene is required for acidic pH activation of cytoplasmic urease

Infection and Immunity

Volumn 68, Issue 2, 2000, Pages 470-477

  Scott, David R ^a,b   Marcus, Elizabeth A ^a,b   Weeks, David L ^a,b   Lee, Adrian ^c   Melchers, Klaus ^d   Sachs, George ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b VA GREATER LOS ANGELES HEALTHCARE SYSTEM   (United States)

^c UNIVERSITY OF NEW SOUTH WALES   (Australia)

^d ALTANA PHARMA AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM PROTEIN; GENE PRODUCT; PROTEIN URE I; UNCLASSIFIED DRUG; UREA; UREASE;

ACIDITY; ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; CONTROLLED STUDY; ENZYME ACTIVATION; GENE EXPRESSION; GRAM NEGATIVE INFECTION; HELICOBACTER PYLORI; NONHUMAN; PRIORITY JOURNAL;

BACTERIAL PROTEINS; BLOTTING, WESTERN; CYTOPLASM; DETERGENTS; ENZYME ACTIVATION; GENES, BACTERIAL; HELICOBACTER PYLORI; HYDROGEN-ION CONCENTRATION; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; POLYMERASE CHAIN REACTION; UREA; UREASE;

EID: 0033958917     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.2.470-477.2000     Document Type: Article

References (31)

1. + on the translocation of charged residues explain the "positive inside" rule. EMBO J. 13:2267-2272.
2. Andrutis, K. A., J. G. Fox, D. B. Schauer, R. P. Marini, J. C. Murphy, L. Yan, and J. V. Solnick. 1995. Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. Infect. Immun. 63:3722-3725.
3. Bauerfeind, P., R. Garner, B. E. Dunn, and H. L. T. Mobley. 1997. Synthesis and activity of Helicobacter pylori urease and catalase at low pH. Gut 40:25-30.
4. Bode, G., P. Malfertheimer, G. Lenhardt, M. Nilius, and H. Dischuneit. 1993. Ultrastructural localization of urease of Helicobacter pylori. Med. Microbiol. Immun. 182:223-242.
5. Chebrou, H., F. Bigey, A. Arnaud, and P. Galzy. 1996. Amide metabolism: a putative ABC transporter in Rhodococcus sp. R312. Gene 182:215-218.
6. Chen, Y.-Y. M., K. A. Clancy, and R. A. Burne. 1996. Streptococcus salivarius urease: genetic and biochemical characterization and expression in a dental plaque streptococcus. Infect. Immun. 64:585-592.
7. Clyne, M., A. Labigne, and B. Drumm. 1995. Helicobacter pylori requires an acidic environment to survive in the presence of urea. Infect. Immun. 63: 1669-1673.
8. Eaton, K. A., and S. Krakowka. 1994. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect. Immun. 62:3604-3607.
9. Goodwin, C. S., and J. Carrick. 1991. Peptic ulcer disease and Helicobacter pylori infection. Curr. Opin. Gastroenterol. 7:108-115.
10. Graham, D. Y. 1989. Campylobacter pylori and peptic ulcer disease. Gastroenterology 96:615-625.
11. Hawtin, P. R., A. R. Stacey, and D. G. Newell. 1990. Investigation of the structure and localization of the urease of Helicobacter pylori using monoclonal antibodies. J. Gen. Microbiol. 136:995-2000.
12. Hediger, M. A., C. P. Smith, G. You, W. S. Lee, Y. Kanai, and C. Shayakul. 1996. Structure, regulation and physiological roles of urea transporters. Kidney Int. 49:1615-1623.
13. Hu, L.-T., and H. L. T. Mobley. 1993. Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli. Infect. Immun. 61:2563-2569.
14. Kashket, E. R. 1985. The proton motive force in bacteria: a critical assessment of methods. Annu. Rev. Microbiol. 39:219-242.
15. Labigne, A., V. Cussac, and P. Courcoux. 1991. Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity. J. Bacteriol 173:1920-1931.
16. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.
17. Marshall, B. J. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet i:1273-1275.
18. Mendz, G. L., and S. L. Hazell. 1996. The urea cycle of Helicobacter pylori. Microbiology 142:2959-2967.
19. McDonald, J. A., K. V. Speeg, Jr., and J. V. Campbell. 1972. Urease, a specific and sensitive radiometric assay. Enzmologia 42:1-9.
20. Meyer-Rosberg, K., D. R. Scott, D. Rex, K. Melchers, and G. Sachs. 1996. The effect of environmental pH on the proton motive force of Helicobacter pylori. Gastroenterology 111:886-900.
21. Mobley, H. L. T., M. D. Island, and R. P. Hausinger. 1995. Molecular biology of microbial ureases. Microbiol. Rev. 59:451-480.
22. Neithercut, W. D., M. A. Greig, M. Hossack, and K. E. L. McColl. 1991. Suicidal destruction of Helicobacter pylori: metabolic consequence of intracellular accumulation of ammonia. J. Clin. Pathol. 44:380-384.
23. Orbach, E., and A. Finkelstein. 1980. The nonelectrolyte permeability of planar lipid bilayer membranes. J. Gen. Physiol. 75:427-436.
24. Phadnis, S. H., M. H. Parlow, M. Levy, D. Liver, C. M. Caulkins, J. B. Conners, and B. E. Dunn. 1995. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial lysis. Infect. Immun. 64:905-912.
25. Rektorschek, M., D. Weeks, G. Sachs, and K. Melchers. 1998. Influence of pH on metabolism and urease activity of Helicobacter pylori. Gastroenterology 115:628-641.
26. Scott, D. R., D. Weeks, C. Hong, S. Postius, K. Melchers, and G. Sachs. 1998. The role of internal urease in acid resistance of Helicobacter pylori. Gastroenterology 114:58-70.
27. Skouloubris, S., J. Thiberge, A. Labigne, and H. De Reuse. 1998. The Helicobacter pylori Urel protein is not involved in urease activity but is essential for bacterial survival in vivo. Infect. Immun. 66:4517-4521.
28. Tsuda, M., M. Karita, M. G. Morshed, K. Okita, and T. Nakazawa. 1994. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagencsis lacks the ability to colonize the nude mouse stomach. Infect. Immun. 62:3586-3589.
29. Wilson, K. 1987. Preparation of genomic DNA from bacteria, p. 2.4.1-2.4.2. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. A. Smith, J. G. Seidman, and K. Struhl (ed.), Current protocols in molecular biology. Wiley, New York, N.Y.
30. Wilson, S. A., R. J. Williams, L. H. Pearl, and R. E. Drew. 1995. Identification of two new genes in the Pseudomonas aeruginosa amidase operon, encoding an ATPase (AmiB) and a putative integral membrane protein (AmiS). J. Biol. Chem. 270:18818-18824.
31. Young, G. M., D. Amid, and V. L. Miller. 1996. A bifunctional urease enhances survival of pathogenic Yersinia enterocolitica and Morganella morganii at low pH. J. Bacteriol. 178:6487-6495.