Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle

Journal of Bacteriology

Volumn 181, Issue 18, 1999, Pages 5615-5623

  Horswill, Alexander R ^a   Escalante Semerena, Jorge C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 METHYLCITRATE DEHYDRATASE; 2 METHYLCITRATE SYNTHASE; 2 METHYLCITRIC ACID; 2 METHYLISOCITRATE HYDRATASE; 2 METHYLISOCITRATE LYASE; CITRATE SYNTHASE; CITRIC ACID; HYDROLYASE; PROPIONATE COENZYME A LIGASE; PROPIONIC ACID; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CATABOLISM; CATALYST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; ESCHERICHIA COLI; FATTY ACID METABOLISM; FATTY ACID OXIDATION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; SALMONELLA TYPHIMURIUM;

ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0032882589     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.18.5615-5623.1999     Document Type: Article

References (41)

1. Aoki, H., H. Uchiyama, H. Umetsu, and T. Tabuchi. 1995. Isolation of 2-methylcitrate dehydratase, a new enzyme serving in the methylcitric acid cycle for propionate metabolism, from Yallowia lipolytica. Biosci. Biotechnol. Biochem. 59:1825-1828.
2. Ausubel, F. A., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology. Greene Publishing Associates & Wiley Interscience, New York, N.Y.
3. Blair, J. M. 1969. Magnesium and aconitase equilibrium: determination of apparent stability constants of magnesium substrate complexes from equilibrium data. Eur. J. Biochem. 8:287-291.
4. Blattner, F. R., G. Plunkett III, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1474.
5. Chan, R. K., D. Botstein, T. Watanabe, and Y. Ogata. 1972. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high transducing lysate. Virology 50:883-898.
6. Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, A. Krogh, J. McLean, S. Moule, L. Murphy, S. Oliver, J. Osborne, M. A. Quail, M. A. Rajandream, J. Rogers, S. Rutter, K. Seeger, S. Skelton, S. Squares, R. Sqares, J. E. Sulston, K. Taylor, S. Whitehead, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
7. Davis, R. W., D. Botstein, and J. R. Roth. 1980. A manual for genetic engineering: advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
8. 13C]propionate oxidation in wild-type and citrate synthase mutant Escherichia coli: evidence for multiple pathways of propionate utilization. Biochem. J. 291:927-932.
9. Fernandez-Briera, A., and A. Garrido-Pertierra. 1988. A degradation pathway of propionate in Salmonella typhimurium LT-2. Biochimie 70:757-768.
10. Gerike, U., D. W. Hough, N. J. Russell, M. L. Dyall-Smith, and M. J. Danson. 1998. Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships. Microbiology 144:929-935.
11. Grimek, T. L., and J. C. Escalante-Semerena. 1999. Unpublished results.
12. Hammelman, T. A., G. A. O'Toole, J. R. Trzebiatowski, A. W. Tsang, D. Rank, and J. C. Escalante-Semerena. 1996. Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2. FEMS Microbiol. Lett. 137:233-239.
13. Horswill, A. R., and J. C. Escalante-Semerena. 1997. Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon. J. Bacteriol. 179:928-940.
14. Horswill, A. R., and J. C. Escalante-Semerena. 1999. The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase. Microbiology 145:1381-1388.
15. Kay, W. W. 1972. Genetic control of the metabolism of propionate by Escherichia coli K12. Biochim. Biophys. Acta 264:508-521.
16. Krebs, H. A. 1953. The equilibrium constants of the fumarase and aconitase systems. Biochem. J. 54:78-82.
17. Kunitz, M. J. 1952. Crystalline inorganic pyrophosphatase isolated from baker's yeast. J. Gen. Physiol. 35:423-450.
18. Laemmli, U. K. 1970. Cleavage and structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Leatherbarrow, R. J. 1998. GraFit version 4.0. Erithacus Software Ltd., Staines, United Kingdom.
20. London, R. E., D. L. Allen, S. A. Gabel, and E. F. DeRose. 1999. Carbon-13 nuclear magnetic resonance study of metabolism of propionate by Escherichia coli. J. Bacteriol. 181:3562-3570.
21. Miyakoshi, S., H. Uchiyama, T. Someya, T. Satoh, and T. Tabuchi. 1987. Distribution of the methylcitric acid cycle and β-oxidation for propionate catabolism in fungi. Agric. Biol. Chem. 51:2381-2387.
22. Mizuno, M., S. Masuda, K. Takemaru, S. Hosono, T. Sato, M. Takeuchi, and Y. Kobayashi. 1996. Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes. Microbiology. 142:3103-3111.
23. Rabin, R., H. C. Reeves, W. S. Wegener, R. E. Megraw, and S. J. Ajl. 1965. Glyoxylate in fatty acid metabolism. Science 150:1548-1558.
24. 13C-labeled coenzyme A esters. Anal. Biochem. 157:84-88.
25. Ryu, J.-I., and R. J. Hartin. 1990. Quick transformation of Salmonella typhimurium LT2. BioTechniques 8:43-45.
26. Saier, M. H., Jr., and T. M. Ramseier. 1996. The catabolite repressor/ activator (Cra) protein of enteric bacteria. J. Bacteriol. 178:3411-3417.
27. Sasse, J. 1991. Detection of proteins, p. 10.6.1-10.6.8. In F. A. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 1. Wiley Interscience, New York, N.Y.
28. s-α-methylisocitrate as substrates. Biochemistry 23:4572-4580.
29. Schmieger, H. 1971. A method for detection of phage mutants with altered transduction ability. Mol. Gen. Genet. 100:378-381.
30. Schmieger, H., and H. Bakhaus. 1973. The origin of DNA in transducing particles of P22 mutants with increased transduction frequencies (HT-mutants). Mol. Gen. Genet. 120:181-190.
31. Srere, P. A., H. Brazil, and L. Gonen. 1963, The citrate condensing enzyme of pigeon breast muscle and moth flight muscle. Acta Chem. Scand. 17:S129-S134.
32. Tabuchi, T., H. Aoki, H. Uchiyama, and T. Nakahara. 1981. 2-Methylcitrate dehydratase, a new enzyme functioning at the methylcitric acid cycle of propionate metabolism. Agric. Biol. Chem. 45:2823-2829.
33. Tabuchi, T., and S. Hara. 1974. Production of 2-methylcitric acid from n-paraffins by mutants of Candida lipolytica. Agric. Biol. Chem. 38:1105-1106.
34. Tabuchi, T., and N. Serizawa. 1975. A hypothetical cyclic pathway for the metabolism of odd-carbon n-alkanes or propionyl-CoA via seven-carbon tricarboxylic acids in yeasts. Agric. Biol. Chem. 39:1055-1061.
35. Textor, S., V. F. Wendisch, A. A. De Graaf, U. Müller, M. I. Linder, D. Linder, and W. Buckel. 1997. Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria. Arch. Microbiol. 168:428-436.
36. Tsai, S. P., R. J. Hartin, and J. Ryu. 1989. Transformation in restriction-deficient Salmonella typhimurium LT2. J. Gen. Microbiol. 135:2561-2567.
37. Tsang, A. W., A. R. Horswill, and J. C. Escalante-Semerena. 1998. Studies of regulation of expression of the propionate (prpBCDE) operon provide insights into how Salmonella typhimurium LT2 integrates its 1,2-propanediol and propionate catabolic pathways. J. Bacteriol. 180:6511-6518.
38. Van Dyk, T. K., and R. A. LaRossa. 1987. Involvement of ack-pta operon products in α-ketobutyrate metabolism in Salmonella typhimurium. Mol. Gen. Genet. 207:435-440.
39. Watson, D., D. L. Lindel, and R. Fall. 1983. Pseudomonas aeruginosa contains an inducible methylcitrate synthase. Curr. Microbiol. 8:17-21.
40. Wegener, W. S., H. C. Reeves, and S. J. Ajl. 1967. Propionate oxidation in Escherichia coli. Arch. Biochem. Biophys. 121:440-442.
41. Wegener, W. S., H. C. Reeves, R. Rabin, and S. J. Ajl. 1968. Alternate pathways of metabolism of short-chain fatty acids. Bacteriol. Rev. 32:1-26.