Early photocycle kinetic behavior of the E46A and Y42F mutants of photoactive yellow protein: Femtosecond spectroscopy

Biophysical Journal

Volumn 81, Issue 4, 2001, Pages 2314-2319

  Devanathan, Savitha ^a   Lin, Su ^b   Cusanovich, Michael A ^a   Woodbury, Neal ^b   Tollin, Gordon ^a  

^a UNIVERSITY OF ARIZONA   (United States)

^b ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GLUTAMINE; PHENYLALANINE; PHOTOACTIVE YELLOW PROTEIN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CHROMATOPHORE; CONTROLLED STUDY; EXCITATION; HYDROGEN BOND; MOLECULAR DYNAMICS; MUTATION; NONHUMAN; PHOTOACTIVATION; PHOTOCHEMISTRY; PROTEIN BINDING; PROTEIN STRUCTURE; SPECTROSCOPY;

BACTERIA (MICROORGANISMS);

EID: 0034805059     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75877-8     Document Type: Article

References (28)

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3. Coupling of hydrogen bonding to chromophore conformation and function in photoactive yellow protein
4. 1 early intermediate of photoactive yellow protein by FTIR spectroscopy
5. Dual photoactive species in Glu46Asp and Glu46Ala mutants of photoactive yellow protein: A pH-driven color transition
6. New insights into the photocycle of Ectothiorhodospira halophila photoactive yellow protein: Photorecovery of the long-lived photobleached intermediate in the Met100Ala mutant
7. Early intermediates in the photocycle of the Glu46Gln mutant of photoactive yellow protein: Femtosecond spectroscopy
8. Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila
9. Solution structure and backbone dynamics of the photoactive yellow protein
10. Structure of a photocycle intermediate by milli-second time-resolved crystallography
11. Active site mutants implicate key residues for control of color and light cycle kinetics of photoactive yellow protein
12. Structure at 0.85 Å resolution of an early protein photocycle intermediate
13. Thiol ester-linked p-coumaric acid as a new photoactive prosthetic group in a protein with rhodopsin-like photochemistry
14. Roles of amino acid residues near the chromophore of photoactive yellow protein
15. Bacterial photoreceptor with similarity to photoactive yellow protein and plant phytochromes
16. Evidence for trans-cis isomerization of the p-coumaric acid chromophore as the photochemical basis of the photocycle of photoactive yellow protein
17. Probing the primary event in the photocycle of photoactive yellow protein using photochemical hole-burning technique
18. Isolation and characterization of soluble cytochromes, ferredoxins and other chromophoric proteins from the halophilic phototrophic bacterium Ectothiorhodospira halophila
19. Transient proton uptake and release is associated with the photocycle of the photoactive yellow protein from the purple bacterium. Ectothiorhodospira halophila
20. Picosecond decay kinetics and quantum yield of fluorescence of the photoactive yellow protein from the halophilic purple phototrophic bacterium Ectothiorhodospira halophila
21. Photoactive yellow protein from the purple phototrophic bacterium. Ectothiorhodospira halophila. Quantum yield of photobleaching and effects of temperature, alcohols, glycerol, and sucrose on kinetics of photobleaching and recovery
22. Properties of a water soluble, yellow protein isolated from a halophilic phototrophic bacterium that has photochemical activity analogous to sensory rhodopsin
23. Functional expression and site-directed mutagenesis of photoactive yellow protein
24. New photocycle intermediates in the photoactive yellow protein from Ectothiorhodospira halophila: Picosecond transient absorption spectroscopy
25. Evidence for a protonated and cis configuration chromophore in the photobleached intermediate of photoactive yellow protein
26. Glu46 donates a proton to the 4-hydroxycinnamate anion chromophore during the photocycle of photoactive yellow protein
27. Formation of a new buried charge drives a large-amplitude protein quake in photoreceptor activation
28. Photocycle dynamics and vibrational spectroscopy of the E46Q mutant of photoactive yellow protein