Early intermediates in the photocycle of the Glu46Gln mutant of photoactive yellow protein: Femtosecond spectroscopy

Biophysical Journal

Volumn 79, Issue 4, 2000, Pages 2132-2137

  Devanathan, Savitha ^a   Lin, Su ^b   Cusanovich, Michael A ^a   Woodbury, Neal ^b   Tollin, Gordon ^a  

^a University of Arizona   (United States)

^b ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN;

ABSORPTION SPECTROSCOPY; ARTICLE; BACTERIUM; KINETICS; NONHUMAN; PHOTOCHEMISTRY; PHOTORECEPTOR; SPECTROSCOPY; TIME;

EID: 0033798658     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(00)76460-5     Document Type: Article

References (7)

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2. Borgstahl, G. E. O., D. R. Williams, and E. D. Getzoff. 1995. 1.4 Å structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore. Biochemistry. 34:6278-6287.
3. Chosrowjan, H., N. Malaga, Y. Shibata, Y. Imamoto, and F. Tokunaga. 1998. Environmental effects on the femtosecond-picosecond fluorescence dynamics of photoactive yellow protein: chromophores in aqueous solution and in protein nanospaces modified by site-directed mutagenesis. J. Phys. Chem. 102:7695-7698.
4. Jiang, Z., L. R. Swem, B. G. Rushing, S. Devanathan, G. Tollin, and C. Bauer. 1999. Bacterial photoreceptor with similarity to photoactive yellow protein and plant phytochromes. Science. 285:406-409.
5. Meyer, T. E. 1985. Isolation and characterization of soluble cytochromes, ferredoxins and other chromophoric proteins from the halophilic phototrophic bacterium Ectothiorhodospira halophila. Biochim. Biophys. Acta. 806:175-183.
6. Meyer, T. E., E. Yakali, M. A. Cusanovich, and G. Tollin. 1987. Properties of a water-soluble, yellow protein isolated from a halophilic phototrophic bacterium that has photochemical activity analogous to sensory rhodopsin. Biochemistry. 26:418-423.
7. Xie, A., W. D. Hoff, A. R. Kroon, and K. J. Hellingwerf. 1996. Glu 46 donates a proton to the 4-hydroxycinnamate anion chromophore during the photocycle of photoactive yellow protein. Biochemistry. 35: 14671-14678.