Structure of a protein photocycle intermediate by millisecond time- resolved crystallography

Science

Volumn 275, Issue 5305, 1997, Pages 1471-1475

  Genick, Ulrich K ^a   Borgstahl, Gloria E O ^a,c   Ng, Kingman ^b,d   Ren, Zhong ^b   Pradervand, Claude ^b   Burke, Patrick M ^a,e   Šrajer, Vukica ^b   Teng, Tsu Yi ^b   Schildkamp, Wilfried ^b   McRee, Duncan E ^a   Moffat, Keith ^b   Getzoff, Elizabeth D ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

^c UNIVERSITY OF TOLEDO   (United States)

^d ELI LILLY AND COMPANY   (United States)

^e UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VISUAL PIGMENT;

ARTICLE; CHROMATOPHORE; CRYSTALLOGRAPHY; ISOMERISM; NONHUMAN; PHOTORECEPTOR; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

BACTERIAL PROTEINS; BINDING SITES; CHROMATIACEAE; CRYSTALLOGRAPHY, X-RAY; ELECTROCHEMISTRY; HYDROGEN BONDING; ISOMERISM; LIGHT; MODELS, MOLECULAR; PHOTORECEPTORS, MICROBIAL; PROTEIN CONFORMATION; SIGNAL TRANSDUCTION; SPECTRUM ANALYSIS;

EID: 0031039399     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5305.1471     Document Type: Article

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55. We thank E. Blum, Y. Chen, A. LeGrand, H. E. Parge, and G. Shea-McCarthy for assistance in designing and constructing apparatus for these experiments and for help with data collection at NSLS, Brookhaven National Laboratory, and previous runs at CHESS, Cornell University; T. Greenhough for help with the Daresbury Laue program suite; J. A. Tainer and M. Baca for valuable discussions; and M. E. Pique for help with AVS figures. Coordinates for the PYP bleached intermediate have been deposited in the Protein Data Bank (accession number 2pyp). Supported by grants from NIH (GM37684 to E.D.G.; GM36452 and RR07707 to K.M.) and the W. M. Keck Foundation (M. Makinen and K.M.), and fellowships from NIH (NRSA GM15820 to G.E.O.B.) and Boehringer Ingelheim Fonds (U.K.G.).